Restriction map for
pF1KE0011
(Linear) (Six-base) MAPSORT of: KIEE0011.seq Check: 4454 from: 1 to: 2439
KIEE0011.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 0 1757 2439
Size: 1757 682
AccI GT'mk_AC
Cuts at: 0 385 2439
Size: 385 2054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1039 1678 1915 1972 2332 2439
Size: 647 392 639 237 57 360 107
Fragments arranged by size:
647 639 392 360 237 107 57
AfeI AGC'GCT
Cuts at: 0 1283 2439
Size: 1283 1156
AlwNI CAG_nnn'CTG
Cuts at: 0 1656 1782 2439
Size: 1656 126 657
Fragments arranged by size:
1656 657 126
ApaLI G'TGCA_C
Cuts at: 0 1936 2439
Size: 1936 503
AvaI C'yCGr_G
Cuts at: 0 1763 2439
Size: 1763 676
BanI G'GyrC_C
Cuts at: 0 253 2439
Size: 253 2186
BanII G_rGCy'C
Cuts at: 0 206 227 997 1458 1817 2439
Size: 206 21 770 461 359 622
Fragments arranged by size:
770 622 461 359 206 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 1477 1649 2439
Size: 1477 172 790
Fragments arranged by size:
1477 790 172
BbvCI CC'TCA_GC
Cuts at: 0 664 1285 1996 2439
Size: 664 621 711 443
Fragments arranged by size:
711 664 621 443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 1289 1716 1750 2439
Size: 1255 34 427 34 689
Fragments arranged by size:
1255 689 427 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 623 1029 2439
Size: 623 406 1410
Fragments arranged by size:
1410 623 406
BlpI GC'TnA_GC
Cuts at: 0 886 1900 2439
Size: 886 1014 539
Fragments arranged by size:
1014 886 539
Bme1580I G_kGCm'C
Cuts at: 0 1940 2439
Size: 1940 499
BmrI ACTGGGnnnn_n'
Cuts at: 0 887 2439
Size: 887 1552
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2424 2439
Size: 2424 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 478 813 1430 1735 1888 1909 2394
Size: 52 426 335 617 305 153 21 485
Cuts at: 2394 2439
Size: 45
Fragments arranged by size:
617 485 426 335 305 153 52 45
21
Bpu10I CC'TnA_GC
Cuts at: 0 664 1285 1996 2439
Size: 664 621 711 443
Fragments arranged by size:
711 664 621 443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1432 1663 2359 2439
Size: 1432 231 696 80
Fragments arranged by size:
1432 696 231 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 641 1406 2350 2439
Size: 641 765 944 89
Fragments arranged by size:
944 765 641 89
BsaHI Gr'CG_yC
Cuts at: 0 1754 2439
Size: 1754 685
BsaWI w'CCGG_w
Cuts at: 0 722 770 2439
Size: 722 48 1669
Fragments arranged by size:
1669 722 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 32 62 2439
Size: 32 30 2377
Fragments arranged by size:
2377 32 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 789 1324 1846 1981 2413 2416 2439
Size: 595 194 535 522 135 432 3 23
Fragments arranged by size:
595 535 522 432 194 135 23 3
BseYI C'CCAG_C
Cuts at: 0 658 871 1853 2058 2439
Size: 658 213 982 205 381
Fragments arranged by size:
982 658 381 213 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 832 1576 2112 2439
Size: 720 112 744 536 327
Fragments arranged by size:
744 720 536 327 112
BsiHKAI G_wGCw'C
Cuts at: 0 206 227 554 892 1178 1458 1817 1906
Size: 206 21 327 338 286 280 359 89
Cuts at: 1906 1940 1949 2439
Size: 34 9 490
Fragments arranged by size:
490 359 338 327 286 280 206 89
34 21 9
BsmI GAATG_Cn'
Cuts at: 0 747 1488 2439
Size: 747 741 951
Fragments arranged by size:
951 747 741
BsmBI CGTCTCn'nnnn_
Cuts at: 0 70 2439
Size: 70 2369
Bsp1286I G_dGCh'C
Cuts at: 0 206 227 554 892 997 1178 1458 1817
Size: 206 21 327 338 105 181 280 359
Cuts at: 1817 1906 1940 1949 2439
Size: 89 34 9 490
Fragments arranged by size:
490 359 338 327 280 206 181 105
89 34 21 9
BspEI T'CCGG_A
Cuts at: 0 770 2439
Size: 770 1669
BspHI T'CATG_A
Cuts at: 0 140 1458 1502 2439
Size: 140 1318 44 937
Fragments arranged by size:
1318 937 140 44
BsrBI CCG'CTC
Cuts at: 0 759 1387 2439
Size: 759 628 1052
Fragments arranged by size:
1052 759 628
BsrDI GCAATG_nn'
Cuts at: 0 215 912 1236 2439
Size: 215 697 324 1203
Fragments arranged by size:
1203 697 324 215
BsrFI r'CCGG_y
Cuts at: 0 764 1543 2439
Size: 764 779 896
Fragments arranged by size:
896 779 764
BssSI C'ACGA_G
Cuts at: 0 316 2439
Size: 316 2123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 444 1986 2439
Size: 444 1542 453
Fragments arranged by size:
1542 453 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1029 1212 2439
Size: 1029 183 1227
Fragments arranged by size:
1227 1029 183
BstYI r'GATC_y
Cuts at: 0 1105 1785 2439
Size: 1105 680 654
Fragments arranged by size:
1105 680 654
BtgI C'CryG_G
Cuts at: 0 389 503 1538 2339 2439
Size: 389 114 1035 801 100
Fragments arranged by size:
1035 801 389 114 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 903 2439
Size: 903 1536
BtsI GCAGTG_nn'
Cuts at: 0 423 1032 1318 1805 2208 2439
Size: 423 609 286 487 403 231
Fragments arranged by size:
609 487 423 403 286 231
ClaI AT'CG_AT
Cuts at: 0 1736 2439
Size: 1736 703
EaeI y'GGCC_r
Cuts at: 0 110 506 600 837 982 1541 1820 1838
Size: 110 396 94 237 145 559 279 18
Cuts at: 1838 2336 2439
Size: 498 103
Fragments arranged by size:
559 498 396 279 237 145 110 103
94 18
EarI CTCTTCn'nnn_
Cuts at: 0 478 623 665 749 2439
Size: 478 145 42 84 1690
Fragments arranged by size:
1690 478 145 84 42
EcoICRI GAG'CTC
Cuts at: 0 204 225 1456 1815 2439
Size: 204 21 1231 359 624
Fragments arranged by size:
1231 624 359 204 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 478 647 813 1039 1430 1678 1735
Size: 52 426 169 166 226 391 248 57
Cuts at: 1735 1888 1909 1915 1972 2332 2394 2439
Size: 153 21 6 57 360 62 45
Fragments arranged by size:
426 391 360 248 226 169 166 153
62 57 57 52 45 21 6
EcoO109I rG'GnC_Cy
Cuts at: 0 90 606 823 1400 1884 2439
Size: 90 516 217 577 484 555
Fragments arranged by size:
577 555 516 484 217 90
HaeII r_GCGC'y
Cuts at: 0 1285 1345 2439
Size: 1285 60 1094
Fragments arranged by size:
1285 1094 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 265 284 297 316 343 375
Size: 228 32 5 19 13 19 27 32
Cuts at: 375 412 444 1097 1129 1770 1802 2038 2070
Size: 37 32 653 32 641 32 236 32
Cuts at: 2070 2439
Size: 369
Fragments arranged by size:
653 641 369 236 228 37 32 32
32 32 32 32 27 19 19 13
5
HincII GTy'rAC
Cuts at: 0 324 2439
Size: 324 2115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1604 2235 2439
Size: 1604 631 204
Fragments arranged by size:
1604 631 204
MscI TGG'CCA
Cuts at: 0 112 1822 2338 2439
Size: 112 1710 516 101
Fragments arranged by size:
1710 516 112 101
MslI CAynn'nnrTG
Cuts at: 0 155 1252 1441 1822 2423 2439
Size: 155 1097 189 381 601 16
Fragments arranged by size:
1097 601 381 189 155 16
MspA1I CmG'CkG
Cuts at: 0 491 1477 1520 1983 2012 2439
Size: 491 986 43 463 29 427
Fragments arranged by size:
986 491 463 427 43 29
NaeI GCC'GGC
Cuts at: 0 1545 2439
Size: 1545 894
NcoI C'CATG_G
Cuts at: 0 389 2339 2439
Size: 389 1950 100
Fragments arranged by size:
1950 389 100
NgoMIV G'CCGG_C
Cuts at: 0 1543 2439
Size: 1543 896
NspI r_CATG'y
Cuts at: 0 1251 2439
Size: 1251 1188
PasI CC'CwG_GG
Cuts at: 0 1562 2439
Size: 1562 877
PflMI CCAn_nnn'nTGG
Cuts at: 0 1782 2439
Size: 1782 657
PfoI T'CCnGG_A
Cuts at: 0 161 749 2439
Size: 161 588 1690
Fragments arranged by size:
1690 588 161
PpuMI rG'GwC_Cy
Cuts at: 0 90 606 823 1400 2439
Size: 90 516 217 577 1039
Fragments arranged by size:
1039 577 516 217 90
PshAI GACnn'nnGTC
Cuts at: 0 361 2439
Size: 361 2078
PstI C_TGCA'G
Cuts at: 0 418 440 496 1552 2229 2439
Size: 418 22 56 1056 677 210
Fragments arranged by size:
1056 677 418 210 56 22
PvuII CAG'CTG
Cuts at: 0 491 1477 1520 2439
Size: 491 986 43 919
Fragments arranged by size:
986 919 491 43
SacI G_AGCT'C
Cuts at: 0 206 227 1458 1817 2439
Size: 206 21 1231 359 622
Fragments arranged by size:
1231 622 359 206 21
SapI GCTCTTCn'nnn_
Cuts at: 0 478 749 2439
Size: 478 271 1690
Fragments arranged by size:
1690 478 271
ScaI AGT'ACT
Cuts at: 0 280 2439
Size: 280 2159
SexAI A'CCwGG_T
Cuts at: 0 1931 2264 2439
Size: 1931 333 175
Fragments arranged by size:
1931 333 175
SfcI C'TryA_G
Cuts at: 0 414 436 492 802 1548 2225 2439
Size: 414 22 56 310 746 677 214
Fragments arranged by size:
746 677 414 310 214 56 22
SmlI C'TyrA_G
Cuts at: 0 1447 1678 2374 2439
Size: 1447 231 696 65
Fragments arranged by size:
1447 696 231 65
SphI G_CATG'C
Cuts at: 0 1251 2439
Size: 1251 1188
StuI AGG'CCT
Cuts at: 0 1495 1927 2439
Size: 1495 432 512
Fragments arranged by size:
1495 512 432
StyI C'CwwG_G
Cuts at: 0 113 389 1211 1490 2180 2339 2439
Size: 113 276 822 279 690 159 100
Fragments arranged by size:
822 690 279 276 159 113 100
TatI w'GTAC_w
Cuts at: 0 278 1301 2439
Size: 278 1023 1138
Fragments arranged by size:
1138 1023 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 1168 1200 1939 1971 2439
Size: 32 32 1104 32 739 32 468
Fragments arranged by size:
1104 739 468 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 957 2439
Size: 957 1482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1716 2262 2439
Size: 1716 546 177
Fragments arranged by size:
1716 546 177
ZraI GAC'GTC
Cuts at: 0 1755 2439
Size: 1755 684
Enzymes that do cut:
AatII AccI AcuI AfeI AlwNI ApaLI AvaI BanI
BanII BbsI BbvCI BcgI BciVI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI ClaI EaeI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI
PshAI PstI PvuII SacI SapI ScaI SexAI SfcI
SmlI SphI StuI StyI TatI TstI Tth111I XcmI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BclI BfrBI BglI BglII BmgBI
BmtI BsaAI BsaBI BsiEI BsiWI BspMI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TaqII XbaI
XhoI XmaI XmnI