Restriction map for
pF1KE0001
(Linear) (Six-base) MAPSORT of: KIEE0001.seq Check: 2246 from: 1 to: 1491
KIEE0001.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 544 1491
Size: 544 947
AccI GT'mk_AC
Cuts at: 0 581 1491
Size: 581 910
Acc65I G'GTAC_C
Cuts at: 0 914 1491
Size: 914 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1491
Size: 794 697
AflIII A'CryG_T
Cuts at: 0 904 1491
Size: 904 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1236 1491
Size: 1236 255
AlwNI CAG_nnn'CTG
Cuts at: 0 339 1491
Size: 339 1152
ApaI G_GGCC'C
Cuts at: 0 308 1491
Size: 308 1183
ApaLI G'TGCA_C
Cuts at: 0 474 1330 1491
Size: 474 856 161
Fragments arranged by size:
856 474 161
AvaI C'yCGr_G
Cuts at: 0 134 426 462 1451 1491
Size: 134 292 36 989 40
Fragments arranged by size:
989 292 134 40 36
BamHI G'GATC_C
Cuts at: 0 55 1016 1491
Size: 55 961 475
Fragments arranged by size:
961 475 55
BanI G'GyrC_C
Cuts at: 0 914 1491
Size: 914 577
BanII G_rGCy'C
Cuts at: 0 308 1275 1411 1491
Size: 308 967 136 80
Fragments arranged by size:
967 308 136 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 1402 1491
Size: 1402 89
BbvCI CC'TCA_GC
Cuts at: 0 1108 1491
Size: 1108 383
BglI GCCn_nnn'nGGC
Cuts at: 0 929 1491
Size: 929 562
BglII A'GATC_T
Cuts at: 0 953 1491
Size: 953 538
BlpI GC'TnA_GC
Cuts at: 0 103 1187 1491
Size: 103 1084 304
Fragments arranged by size:
1084 304 103
Bme1580I G_kGCm'C
Cuts at: 0 308 478 664 925 1198 1334 1491
Size: 308 170 186 261 273 136 157
Fragments arranged by size:
308 273 261 186 170 157 136
BmgBI CAC'GTC
Cuts at: 0 1368 1374 1491
Size: 1368 6 117
Fragments arranged by size:
1368 117 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 360 1278 1491
Size: 360 918 213
Fragments arranged by size:
918 360 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 477 1491
Size: 147 330 1014
Fragments arranged by size:
1014 330 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1491
Size: 277 1214
Bpu10I CC'TnA_GC
Cuts at: 0 1108 1414 1491
Size: 1108 306 77
Fragments arranged by size:
1108 306 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1293 1491
Size: 1293 198
BsaI GGTCTCn'nnnn_
Cuts at: 0 804 1491
Size: 804 687
BsaAI yAC'GTr
Cuts at: 0 905 1404 1491
Size: 905 499 87
Fragments arranged by size:
905 499 87
BsaWI w'CCGG_w
Cuts at: 0 1275 1469 1491
Size: 1275 194 22
Fragments arranged by size:
1275 194 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 705 735 1025 1055 1491
Size: 705 30 290 30 436
Fragments arranged by size:
705 436 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 184 1439 1491
Size: 109 75 1255 52
Fragments arranged by size:
1255 109 75 52
BseYI C'CCAG_C
Cuts at: 0 667 924 1491
Size: 667 257 567
Fragments arranged by size:
667 567 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 229 403 1120 1177 1491
Size: 169 60 174 717 57 314
Fragments arranged by size:
717 314 174 169 60 57
BsiEI CG_ry'CG
Cuts at: 0 1181 1491
Size: 1181 310
BsiHKAI G_wGCw'C
Cuts at: 0 302 463 478 1334 1491
Size: 302 161 15 856 157
Fragments arranged by size:
856 302 161 157 15
Bsp1286I G_dGCh'C
Cuts at: 0 302 308 463 478 664 925 1198 1275
Size: 302 6 155 15 186 261 273 77
Cuts at: 1275 1334 1411 1491
Size: 59 77 80
Fragments arranged by size:
302 273 261 186 155 80 77 77
59 15 6
BspEI T'CCGG_A
Cuts at: 0 1469 1491
Size: 1469 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 544 1491
Size: 347 197 947
Fragments arranged by size:
947 347 197
BsrFI r'CCGG_y
Cuts at: 0 644 1491
Size: 644 847
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 1491
Size: 318 1173
BstYI r'GATC_y
Cuts at: 0 55 173 953 1016 1472 1491
Size: 55 118 780 63 456 19
Fragments arranged by size:
780 456 118 63 55 19
Bsu36I CC'TnA_GG
Cuts at: 0 215 1491
Size: 215 1276
BtgI C'CryG_G
Cuts at: 0 1382 1491
Size: 1382 109
BtsI GCAGTG_nn'
Cuts at: 0 1218 1245 1491
Size: 1218 27 246
Fragments arranged by size:
1218 246 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1338 1373 1491
Size: 1338 35 118
Fragments arranged by size:
1338 118 35
DraIII CAC_nnn'GTG
Cuts at: 0 1098 1491
Size: 1098 393
EaeI y'GGCC_r
Cuts at: 0 642 1491
Size: 642 849
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 794 1491
Size: 277 517 697
Fragments arranged by size:
697 517 277
EcoO109I rG'GnC_Cy
Cuts at: 0 466 1065 1491
Size: 466 599 426
Fragments arranged by size:
599 466 426
EcoRV GAT'ATC
Cuts at: 0 1047 1491
Size: 1047 444
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1086 1491
Size: 1086 405
FseI GG_CCGG'CC
Cuts at: 0 648 1491
Size: 648 843
HaeII r_GCGC'y
Cuts at: 0 347 1491
Size: 347 1144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1170 1202 1491
Size: 1170 32 289
Fragments arranged by size:
1170 289 32
HincII GTy'rAC
Cuts at: 0 843 1491
Size: 843 648
HindIII A'AGCT_T
Cuts at: 0 193 1491
Size: 193 1298
KpnI G_GTAC'C
Cuts at: 0 918 1491
Size: 918 573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 819 1491
Size: 248 571 672
Fragments arranged by size:
672 571 248
MslI CAynn'nnrTG
Cuts at: 0 877 1491
Size: 877 614
MspA1I CmG'CkG
Cuts at: 0 683 1191 1491
Size: 683 508 300
Fragments arranged by size:
683 508 300
NaeI GCC'GGC
Cuts at: 0 646 1491
Size: 646 845
NdeI CA'TA_TG
Cuts at: 0 991 1491
Size: 991 500
NgoMIV G'CCGG_C
Cuts at: 0 644 1491
Size: 644 847
PasI CC'CwG_GG
Cuts at: 0 155 1491
Size: 155 1336
PfoI T'CCnGG_A
Cuts at: 0 1019 1491
Size: 1019 472
PmlI CAC'GTG
Cuts at: 0 905 1491
Size: 905 586
PpuMI rG'GwC_Cy
Cuts at: 0 1065 1491
Size: 1065 426
PshAI GACnn'nnGTC
Cuts at: 0 856 1491
Size: 856 635
PspOMI G'GGCC_C
Cuts at: 0 304 1491
Size: 304 1187
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 565 597 1491
Size: 565 32 894
Fragments arranged by size:
894 565 32
PstI C_TGCA'G
Cuts at: 0 273 688 1491
Size: 273 415 803
Fragments arranged by size:
803 415 273
PvuII CAG'CTG
Cuts at: 0 683 1191 1491
Size: 683 508 300
Fragments arranged by size:
683 508 300
SfcI C'TryA_G
Cuts at: 0 269 684 1491
Size: 269 415 807
Fragments arranged by size:
807 415 269
SmaI CCC'GGG
Cuts at: 0 136 1491
Size: 136 1355
SmlI C'TyrA_G
Cuts at: 0 1308 1491
Size: 1308 183
StuI AGG'CCT
Cuts at: 0 220 1037 1465 1491
Size: 220 817 428 26
Fragments arranged by size:
817 428 220 26
StyI C'CwwG_G
Cuts at: 0 29 60 1491
Size: 29 31 1431
Fragments arranged by size:
1431 31 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4 681 1491
Size: 4 677 810
Fragments arranged by size:
810 677 4
TatI w'GTAC_w
Cuts at: 0 233 1491
Size: 233 1258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1025 1057 1491
Size: 1025 32 434
Fragments arranged by size:
1025 434 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 611 1134 1491
Size: 315 296 523 357
Fragments arranged by size:
523 357 315 296
XmaI C'CCGG_G
Cuts at: 0 134 1491
Size: 134 1357
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstXI BstYI
Bsu36I BtgI BtsI CspCI DraIII EaeI Eco57MI EcoO109I
EcoRV FalI FseI HaeII Hin4I HincII HindIII KpnI
MmeI MslI MspA1I NaeI NdeI NgoMIV PasI PfoI
PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SfcI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BmtI BsaBI BsaHI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI DraI DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI FspI FspAI HpaI
KasI MfeI MluI MscI NarI NcoI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PpiI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI ZraI