Restriction map for
pF1KB9995
(Linear) (Six-base) MAPSORT of: KIBB9995.seq Check: 7913 from: 1 to: 1755
KIBB9995.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1755
Size: 340 1415
AflIII A'CryG_T
Cuts at: 0 266 608 740 1755
Size: 266 342 132 1015
Fragments arranged by size:
1015 342 266 132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1106 1229 1276 1755
Size: 1106 123 47 479
Fragments arranged by size:
1106 479 123 47
ApoI r'AATT_y
Cuts at: 0 462 473 1755
Size: 462 11 1282
Fragments arranged by size:
1282 462 11
BanI G'GyrC_C
Cuts at: 0 86 727 1755
Size: 86 641 1028
Fragments arranged by size:
1028 641 86
BanII G_rGCy'C
Cuts at: 0 16 700 1755
Size: 16 684 1055
Fragments arranged by size:
1055 684 16
BbeI G_GCGC'C
Cuts at: 0 731 1755
Size: 731 1024
BbsI GAAGACnn'nnnn_
Cuts at: 0 1556 1755
Size: 1556 199
BbvCI CC'TCA_GC
Cuts at: 0 1496 1755
Size: 1496 259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 68 102 1755
Size: 68 34 1653
Fragments arranged by size:
1653 68 34
BfrBI ATG'CAT
Cuts at: 0 483 1083 1263 1755
Size: 483 600 180 492
Fragments arranged by size:
600 492 483 180
BglI GCCn_nnn'nGGC
Cuts at: 0 208 1755
Size: 208 1547
BglII A'GATC_T
Cuts at: 0 1073 1755
Size: 1073 682
BmgBI CAC'GTC
Cuts at: 0 830 1172 1755
Size: 830 342 583
Fragments arranged by size:
830 583 342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1337 1574 1755
Size: 614 723 237 181
Fragments arranged by size:
723 614 237 181
Bpu10I CC'TnA_GC
Cuts at: 0 1496 1755
Size: 1496 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1755
Size: 838 917
BsaHI Gr'CG_yC
Cuts at: 0 152 728 1341 1755
Size: 152 576 613 414
Fragments arranged by size:
613 576 414 152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1643 1673 1755
Size: 1643 30 82
Fragments arranged by size:
1643 82 30
BseYI C'CCAG_C
Cuts at: 0 1689 1755
Size: 1689 66
BsiHKAI G_wGCw'C
Cuts at: 0 1213 1755
Size: 1213 542
BsmBI CGTCTCn'nnnn_
Cuts at: 0 143 1755
Size: 143 1612
Bsp1286I G_dGCh'C
Cuts at: 0 16 700 1213 1755
Size: 16 684 513 542
Fragments arranged by size:
684 542 513 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 1755
Size: 296 1459
BsrBI CCG'CTC
Cuts at: 0 173 1755
Size: 173 1582
BsrDI GCAATG_nn'
Cuts at: 0 483 818 1755
Size: 483 335 937
Fragments arranged by size:
937 483 335
BsrFI r'CCGG_y
Cuts at: 0 162 294 1755
Size: 162 132 1461
Fragments arranged by size:
1461 162 132
BssSI C'ACGA_G
Cuts at: 0 991 1755
Size: 991 764
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1611 1755
Size: 1611 144
BstYI r'GATC_y
Cuts at: 0 1073 1755
Size: 1073 682
Bsu36I CC'TnA_GG
Cuts at: 0 873 1755
Size: 873 882
BtgI C'CryG_G
Cuts at: 0 190 1755
Size: 190 1565
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 719 1755
Size: 719 1036
BtsI GCAGTG_nn'
Cuts at: 0 1213 1755
Size: 1213 542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 446 481 1755
Size: 446 35 1274
Fragments arranged by size:
1274 446 35
DraI TTT'AAA
Cuts at: 0 1359 1755
Size: 1359 396
EaeI y'GGCC_r
Cuts at: 0 688 1755
Size: 688 1067
EarI CTCTTCn'nnn_
Cuts at: 0 812 1226 1553 1755
Size: 812 414 327 202
Fragments arranged by size:
812 414 327 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 181 1755
Size: 181 1574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 614 1337 1574 1755
Size: 340 274 723 237 181
Fragments arranged by size:
723 340 274 237 181
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1579 1755
Size: 1579 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 335 1755
Size: 335 1420
HaeII r_GCGC'y
Cuts at: 0 731 1755
Size: 731 1024
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 702 734 1161 1193 1554 1586 1643 1675
Size: 702 32 427 32 361 32 57 32
Cuts at: 1675 1755
Size: 80
Fragments arranged by size:
702 427 361 80 57 32 32 32
32
HincII GTy'rAC
Cuts at: 0 261 1755
Size: 261 1494
HindIII A'AGCT_T
Cuts at: 0 1533 1755
Size: 1533 222
KasI G'GCGC_C
Cuts at: 0 727 1755
Size: 727 1028
MluI A'CGCG_T
Cuts at: 0 266 1755
Size: 266 1489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 546 1755
Size: 17 529 1209
Fragments arranged by size:
1209 529 17
MscI TGG'CCA
Cuts at: 0 690 1755
Size: 690 1065
MslI CAynn'nnrTG
Cuts at: 0 1088 1349 1505 1755
Size: 1088 261 156 250
Fragments arranged by size:
1088 261 250 156
MspA1I CmG'CkG
Cuts at: 0 192 242 1006 1693 1755
Size: 192 50 764 687 62
Fragments arranged by size:
764 687 192 62 50
NaeI GCC'GGC
Cuts at: 0 164 296 1755
Size: 164 132 1459
Fragments arranged by size:
1459 164 132
NarI GG'CG_CC
Cuts at: 0 728 1755
Size: 728 1027
NgoMIV G'CCGG_C
Cuts at: 0 162 294 1755
Size: 162 132 1461
Fragments arranged by size:
1461 162 132
NsiI A_TGCA'T
Cuts at: 0 485 1085 1265 1755
Size: 485 600 180 490
Fragments arranged by size:
600 490 485 180
NspI r_CATG'y
Cuts at: 0 612 744 1087 1267 1411 1755
Size: 612 132 343 180 144 344
Fragments arranged by size:
612 344 343 180 144 132
PciI A'CATG_T
Cuts at: 0 608 740 1755
Size: 608 132 1015
Fragments arranged by size:
1015 608 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 1647 1755
Size: 1647 108
PstI C_TGCA'G
Cuts at: 0 241 870 1755
Size: 241 629 885
Fragments arranged by size:
885 629 241
PvuII CAG'CTG
Cuts at: 0 242 1006 1755
Size: 242 764 749
Fragments arranged by size:
764 749 242
SacII CC_GC'GG
Cuts at: 0 193 1755
Size: 193 1562
SapI GCTCTTCn'nnn_
Cuts at: 0 812 1755
Size: 812 943
SfcI C'TryA_G
Cuts at: 0 237 866 1755
Size: 237 629 889
Fragments arranged by size:
889 629 237
SfoI GGC'GCC
Cuts at: 0 729 1755
Size: 729 1026
SmlI C'TyrA_G
Cuts at: 0 853 1755
Size: 853 902
SphI G_CATG'C
Cuts at: 0 1267 1755
Size: 1267 488
StuI AGG'CCT
Cuts at: 0 1327 1755
Size: 1327 428
StyI C'CwwG_G
Cuts at: 0 1646 1740 1755
Size: 1646 94 15
Fragments arranged by size:
1646 94 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1001 1033 1128 1160 1755
Size: 1001 32 95 32 595
Fragments arranged by size:
1001 595 95 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1657 1755
Size: 1657 98
XmnI GAAnn'nnTTC
Cuts at: 0 373 1755
Size: 373 1382
Enzymes that do cut:
AcuI AflIII AlfI ApoI BanI BanII BbeI BbsI
BbvCI BcgI BfrBI BglI BglII BmgBI BpmI Bpu10I
BpuEI BsaHI BsaXI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI EaeI EarI EciI Eco57MI
EcoNI FalI HaeII Hin4I HincII HindIII KasI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PflMI PstI PvuII SacII SapI SfcI
SfoI SmlI SphI StuI StyI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BciVI BclI BlpI
Bme1580I BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI
BseRI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I ClaI DraIII DrdI
EagI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI
HpaI KpnI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TaqII TatI XbaI
XcmI XhoI XmaI ZraI