Restriction map for
pF1KB9987
(Linear) (Six-base) MAPSORT of: KIBB9987.seq Check: 5074 from: 1 to: 1722
KIBB9987.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 138 277 1722
Size: 138 139 1445
Fragments arranged by size:
1445 139 138
AccI GT'mk_AC
Cuts at: 0 1613 1722
Size: 1613 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1582 1690 1722
Size: 1582 108 32
Fragments arranged by size:
1582 108 32
AfeI AGC'GCT
Cuts at: 0 1372 1722
Size: 1372 350
AleI CACnn'nnGTG
Cuts at: 0 685 1722
Size: 685 1037
AlwNI CAG_nnn'CTG
Cuts at: 0 84 311 777 921 986 1156 1722
Size: 84 227 466 144 65 170 566
Fragments arranged by size:
566 466 227 170 144 84 65
ApaLI G'TGCA_C
Cuts at: 0 767 1722
Size: 767 955
ApoI r'AATT_y
Cuts at: 0 1276 1536 1722
Size: 1276 260 186
Fragments arranged by size:
1276 260 186
AvaI C'yCGr_G
Cuts at: 0 872 1722
Size: 872 850
BanI G'GyrC_C
Cuts at: 0 153 849 1043 1722
Size: 153 696 194 679
Fragments arranged by size:
696 679 194 153
BanII G_rGCy'C
Cuts at: 0 399 1722
Size: 399 1323
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 1722
Size: 308 1414
BbvCI CC'TCA_GC
Cuts at: 0 8 525 1722
Size: 8 517 1197
Fragments arranged by size:
1197 517 8
BciVI GTATCCnnnnn_n'
Cuts at: 0 264 767 1722
Size: 264 503 955
Fragments arranged by size:
955 503 264
BglI GCCn_nnn'nGGC
Cuts at: 0 1593 1722
Size: 1593 129
Bme1580I G_kGCm'C
Cuts at: 0 771 1111 1589 1722
Size: 771 340 478 133
Fragments arranged by size:
771 478 340 133
BmgBI CAC'GTC
Cuts at: 0 1434 1722
Size: 1434 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 947 1166 1722
Size: 310 637 219 556
Fragments arranged by size:
637 556 310 219
Bpu10I CC'TnA_GC
Cuts at: 0 8 525 1722
Size: 8 517 1197
Fragments arranged by size:
1197 517 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 266 1722
Size: 194 72 1456
Fragments arranged by size:
1456 194 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 1116 1722
Size: 1116 606
BsaBI GATnn'nnATC
Cuts at: 0 1399 1722
Size: 1399 323
BsaWI w'CCGG_w
Cuts at: 0 196 1722
Size: 196 1526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 920 950 1722
Size: 178 30 712 30 772
Fragments arranged by size:
772 712 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1670 1722
Size: 1670 52
BseYI C'CCAG_C
Cuts at: 0 569 629 979 1136 1149 1722
Size: 569 60 350 157 13 573
Fragments arranged by size:
573 569 350 157 60 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 931 976 1722
Size: 155 776 45 746
Fragments arranged by size:
776 746 155 45
BsiHKAI G_wGCw'C
Cuts at: 0 358 771 1089 1559 1722
Size: 358 413 318 470 163
Fragments arranged by size:
470 413 358 318 163
BsmI GAATG_Cn'
Cuts at: 0 38 1722
Size: 38 1684
Bsp1286I G_dGCh'C
Cuts at: 0 358 399 771 1089 1111 1559 1589 1722
Size: 358 41 372 318 22 448 30 133
Fragments arranged by size:
448 372 358 318 133 41 30 22
BspEI T'CCGG_A
Cuts at: 0 196 1722
Size: 196 1526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 252 277 1722
Size: 138 114 25 1445
Fragments arranged by size:
1445 138 114 25
BsrFI r'CCGG_y
Cuts at: 0 900 1722
Size: 900 822
BstAPI GCAn_nnn'nTGC
Cuts at: 0 921 954 1722
Size: 921 33 768
Fragments arranged by size:
921 768 33
BstEII G'GTnAC_C
Cuts at: 0 264 1722
Size: 264 1458
BstXI CCAn_nnnn'nTGG
Cuts at: 0 829 1722
Size: 829 893
BstYI r'GATC_y
Cuts at: 0 597 1722
Size: 597 1125
Bsu36I CC'TnA_GG
Cuts at: 0 1378 1722
Size: 1378 344
BtgI C'CryG_G
Cuts at: 0 805 1684 1722
Size: 805 879 38
Fragments arranged by size:
879 805 38
DraIII CAC_nnn'GTG
Cuts at: 0 849 954 1722
Size: 849 105 768
Fragments arranged by size:
849 768 105
EaeI y'GGCC_r
Cuts at: 0 483 858 1722
Size: 483 375 864
Fragments arranged by size:
864 483 375
EarI CTCTTCn'nnn_
Cuts at: 0 107 992 1564 1661 1722
Size: 107 885 572 97 61
Fragments arranged by size:
885 572 107 97 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 947 1166 1582 1690 1722
Size: 310 637 219 416 108 32
Fragments arranged by size:
637 416 310 219 108 32
EcoO109I rG'GnC_Cy
Cuts at: 0 668 702 1690 1722
Size: 668 34 988 32
Fragments arranged by size:
988 668 34 32
EcoRV GAT'ATC
Cuts at: 0 1353 1722
Size: 1353 369
FspI TGC'GCA
Cuts at: 0 277 1722
Size: 277 1445
HaeII r_GCGC'y
Cuts at: 0 1374 1445 1722
Size: 1374 71 277
Fragments arranged by size:
1374 277 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 765 797 1722
Size: 361 32 372 32 925
Fragments arranged by size:
925 372 361 32 32
HindIII A'AGCT_T
Cuts at: 0 338 1722
Size: 338 1384
MfeI C'AATT_G
Cuts at: 0 1316 1722
Size: 1316 406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 1722
Size: 372 1350
MscI TGG'CCA
Cuts at: 0 485 860 1722
Size: 485 375 862
Fragments arranged by size:
862 485 375
MslI CAynn'nnrTG
Cuts at: 0 43 685 1261 1552 1722
Size: 43 642 576 291 170
Fragments arranged by size:
642 576 291 170 43
MspA1I CmG'CkG
Cuts at: 0 246 466 660 1153 1455 1619 1722
Size: 246 220 194 493 302 164 103
Fragments arranged by size:
493 302 246 220 194 164 103
NaeI GCC'GGC
Cuts at: 0 902 1722
Size: 902 820
NcoI C'CATG_G
Cuts at: 0 805 1722
Size: 805 917
NdeI CA'TA_TG
Cuts at: 0 855 1722
Size: 855 867
NgoMIV G'CCGG_C
Cuts at: 0 900 1722
Size: 900 822
NspI r_CATG'y
Cuts at: 0 767 802 1585 1722
Size: 767 35 783 137
Fragments arranged by size:
783 767 137 35
PasI CC'CwG_GG
Cuts at: 0 986 1722
Size: 986 736
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 986 1722
Size: 555 431 736
Fragments arranged by size:
736 555 431
PfoI T'CCnGG_A
Cuts at: 0 828 1722
Size: 828 894
PpuMI rG'GwC_Cy
Cuts at: 0 702 1722
Size: 702 1020
PspXI vC'TCGA_Gb
Cuts at: 0 872 1722
Size: 872 850
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 802 834 1722
Size: 802 32 888
Fragments arranged by size:
888 802 32
PstI C_TGCA'G
Cuts at: 0 316 363 408 1116 1722
Size: 316 47 45 708 606
Fragments arranged by size:
708 606 316 47 45
PvuII CAG'CTG
Cuts at: 0 660 1153 1455 1619 1722
Size: 660 493 302 164 103
Fragments arranged by size:
660 493 302 164 103
SapI GCTCTTCn'nnn_
Cuts at: 0 1564 1722
Size: 1564 158
SexAI A'CCwGG_T
Cuts at: 0 845 1722
Size: 845 877
SfcI C'TryA_G
Cuts at: 0 312 359 404 1112 1614 1722
Size: 312 47 45 708 502 108
Fragments arranged by size:
708 502 312 108 47 45
SmlI C'TyrA_G
Cuts at: 0 209 281 872 1722
Size: 209 72 591 850
Fragments arranged by size:
850 591 209 72
SspI AAT'ATT
Cuts at: 0 103 1722
Size: 103 1619
StuI AGG'CCT
Cuts at: 0 329 593 944 1722
Size: 329 264 351 778
Fragments arranged by size:
778 351 329 264
StyI C'CwwG_G
Cuts at: 0 536 805 1039 1100 1722
Size: 536 269 234 61 622
Fragments arranged by size:
622 536 269 234 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 171 189 338 341 1722
Size: 171 18 149 3 1381
Fragments arranged by size:
1381 171 149 18 3
TatI w'GTAC_w
Cuts at: 0 1487 1722
Size: 1487 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 178 210 920 952 1722
Size: 178 32 710 32 770
Fragments arranged by size:
770 710 178 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 803 853 1707 1722
Size: 803 50 854 15
Fragments arranged by size:
854 803 50 15
XhoI C'TCGA_G
Cuts at: 0 872 1722
Size: 872 850
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BciVI BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI DraIII
EaeI EarI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I
HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PspXI
PsrI PstI PvuII SapI SexAI SfcI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BclI BfrBI BglII BlpI BmrI BmtI
BplI BsaAI BsaHI BsiEI BsiWI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI
EcoRI FalI FseI FspAI HincII HpaI KasI KpnI
MluI NarI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XmaI XmnI ZraI