Restriction map for
pF1KB9984
(Linear) (Six-base) MAPSORT of: KIBB9984.seq Check: 3798 from: 1 to: 2133
KIBB9984.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 0 302 2133
Size: 302 1831
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 612 2133
Size: 415 197 1521
Fragments arranged by size:
1521 415 197
AhdI GACnn_n'nnGTC
Cuts at: 0 455 2133
Size: 455 1678
AleI CACnn'nnGTG
Cuts at: 0 1444 2133
Size: 1444 689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1291 1323 2133
Size: 1291 32 810
Fragments arranged by size:
1291 810 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1122 1896 2133
Size: 1122 774 237
Fragments arranged by size:
1122 774 237
ApaI G_GGCC'C
Cuts at: 0 39 2133
Size: 39 2094
ApaLI G'TGCA_C
Cuts at: 0 84 2133
Size: 84 2049
ApoI r'AATT_y
Cuts at: 0 913 1510 1915 2119 2133
Size: 913 597 405 204 14
Fragments arranged by size:
913 597 405 204 14
AvaI C'yCGr_G
Cuts at: 0 31 100 803 993 1554 2133
Size: 31 69 703 190 561 579
Fragments arranged by size:
703 579 561 190 69 31
BanI G'GyrC_C
Cuts at: 0 640 987 1281 1658 2133
Size: 640 347 294 377 475
Fragments arranged by size:
640 475 377 347 294
BanII G_rGCy'C
Cuts at: 0 39 491 1504 2133
Size: 39 452 1013 629
Fragments arranged by size:
1013 629 452 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 3 2133
Size: 3 2130
BciVI GTATCCnnnnn_n'
Cuts at: 0 102 205 2133
Size: 102 103 1928
Fragments arranged by size:
1928 103 102
BglI GCCn_nnn'nGGC
Cuts at: 0 410 826 1790 2133
Size: 410 416 964 343
Fragments arranged by size:
964 416 410 343
BglII A'GATC_T
Cuts at: 0 948 1558 2133
Size: 948 610 575
Fragments arranged by size:
948 610 575
Bme1580I G_kGCm'C
Cuts at: 0 39 88 992 1542 1616 2133
Size: 39 49 904 550 74 517
Fragments arranged by size:
904 550 517 74 49 39
BmgBI CAC'GTC
Cuts at: 0 675 2133
Size: 675 1458
BmrI ACTGGGnnnn_n'
Cuts at: 0 1655 2133
Size: 1655 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1697 2133
Size: 689 1008 436
Fragments arranged by size:
1008 689 436
Bpu10I CC'TnA_GC
Cuts at: 0 485 1748 2133
Size: 485 1263 385
Fragments arranged by size:
1263 485 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1170 2133
Size: 1071 99 963
Fragments arranged by size:
1071 963 99
BsaWI w'CCGG_w
Cuts at: 0 1655 2133
Size: 1655 478
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 57 87 1006 1036 1238 1268 2133
Size: 57 30 919 30 202 30 865
Fragments arranged by size:
919 865 202 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 600 1078 1164 2133
Size: 600 478 86 969
Fragments arranged by size:
969 600 478 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 2133
Size: 560 1573
BsiHKAI G_wGCw'C
Cuts at: 0 88 697 1575 2092 2133
Size: 88 609 878 517 41
Fragments arranged by size:
878 609 517 88 41
BsmI GAATG_Cn'
Cuts at: 0 1677 2133
Size: 1677 456
BsmBI CGTCTCn'nnnn_
Cuts at: 0 653 665 1673 2133
Size: 653 12 1008 460
Fragments arranged by size:
1008 653 460 12
Bsp1286I G_dGCh'C
Cuts at: 0 39 88 491 697 992 1504 1542 1575
Size: 39 49 403 206 295 512 38 33
Cuts at: 1575 1616 2092 2133
Size: 41 476 41
Fragments arranged by size:
512 476 403 295 206 49 41 41
39 38 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1177 2133
Size: 1177 956
BsrDI GCAATG_nn'
Cuts at: 0 203 1199 1629 2133
Size: 203 996 430 504
Fragments arranged by size:
996 504 430 203
BsrGI T'GTAC_A
Cuts at: 0 1256 2133
Size: 1256 877
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1122 1771 2133
Size: 1122 649 362
Fragments arranged by size:
1122 649 362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 925 1144 1239 2133
Size: 925 219 95 894
Fragments arranged by size:
925 894 219 95
BstYI r'GATC_y
Cuts at: 0 948 1558 1930 2133
Size: 948 610 372 203
Fragments arranged by size:
948 610 372 203
Bsu36I CC'TnA_GG
Cuts at: 0 2125 2133
Size: 2125 8
BtgI C'CryG_G
Cuts at: 0 341 1445 1475 1835 1853 2019 2133
Size: 341 1104 30 360 18 166 114
Fragments arranged by size:
1104 360 341 166 114 30 18
BtsI GCAGTG_nn'
Cuts at: 0 1034 1167 1259 1326 2133
Size: 1034 133 92 67 807
Fragments arranged by size:
1034 807 133 92 67
DraIII CAC_nnn'GTG
Cuts at: 0 835 2003 2133
Size: 835 1168 130
Fragments arranged by size:
1168 835 130
EaeI y'GGCC_r
Cuts at: 0 57 506 2009 2133
Size: 57 449 1503 124
Fragments arranged by size:
1503 449 124 57
EarI CTCTTCn'nnn_
Cuts at: 0 135 1492 2133
Size: 135 1357 641
Fragments arranged by size:
1357 641 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 310 862 2133
Size: 310 552 1271
Fragments arranged by size:
1271 552 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 612 689 1697 2133
Size: 415 197 77 1008 436
Fragments arranged by size:
1008 436 415 197 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1355 1888 2133
Size: 1355 533 245
Fragments arranged by size:
1355 533 245
EcoO109I rG'GnC_Cy
Cuts at: 0 35 36 708 807 2133
Size: 35 1 672 99 1326
Fragments arranged by size:
1326 672 99 35 1
EcoRI G'AATT_C
Cuts at: 0 2119 2133
Size: 2119 14
EcoRV GAT'ATC
Cuts at: 0 1363 2133
Size: 1363 770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1381 1971 2133
Size: 1381 590 162
Fragments arranged by size:
1381 590 162
FspI TGC'GCA
Cuts at: 0 1126 2133
Size: 1126 1007
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 422 441 454 473 697 729 1543 1548
Size: 422 19 13 19 224 32 814 5
Cuts at: 1548 1550 1575 1580 1582 2133
Size: 2 25 5 2 551
Fragments arranged by size:
814 551 422 224 32 25 19 19
13 5 5 2 2
MscI TGG'CCA
Cuts at: 0 508 2011 2133
Size: 508 1503 122
Fragments arranged by size:
1503 508 122
MslI CAynn'nnrTG
Cuts at: 0 335 1444 2133
Size: 335 1109 689
Fragments arranged by size:
1109 689 335
MspA1I CmG'CkG
Cuts at: 0 164 375 427 502 527 2133
Size: 164 211 52 75 25 1606
Fragments arranged by size:
1606 211 164 75 52 25
NcoI C'CATG_G
Cuts at: 0 1475 1835 2019 2133
Size: 1475 360 184 114
Fragments arranged by size:
1475 360 184 114
NdeI CA'TA_TG
Cuts at: 0 2034 2133
Size: 2034 99
PasI CC'CwG_GG
Cuts at: 0 1543 2133
Size: 1543 590
PfoI T'CCnGG_A
Cuts at: 0 2105 2133
Size: 2105 28
PpuMI rG'GwC_Cy
Cuts at: 0 708 807 2133
Size: 708 99 1326
Fragments arranged by size:
1326 708 99
PspOMI G'GGCC_C
Cuts at: 0 35 2133
Size: 35 2098
PstI C_TGCA'G
Cuts at: 0 1266 1461 2133
Size: 1266 195 672
Fragments arranged by size:
1266 672 195
PvuII CAG'CTG
Cuts at: 0 164 375 502 527 2133
Size: 164 211 127 25 1606
Fragments arranged by size:
1606 211 164 127 25
SanDI GG'GwC_CC
Cuts at: 0 807 2133
Size: 807 1326
SapI GCTCTTCn'nnn_
Cuts at: 0 1492 2133
Size: 1492 641
SfcI C'TryA_G
Cuts at: 0 287 443 1262 1457 2133
Size: 287 156 819 195 676
Fragments arranged by size:
819 676 287 195 156
SmaI CCC'GGG
Cuts at: 0 805 2133
Size: 805 1328
SmlI C'TyrA_G
Cuts at: 0 1050 1149 2133
Size: 1050 99 984
Fragments arranged by size:
1050 984 99
SspI AAT'ATT
Cuts at: 0 1522 2044 2133
Size: 1522 522 89
Fragments arranged by size:
1522 522 89
StuI AGG'CCT
Cuts at: 0 263 413 2133
Size: 263 150 1720
Fragments arranged by size:
1720 263 150
StyI C'CwwG_G
Cuts at: 0 1017 1475 1835 2019 2133
Size: 1017 458 360 184 114
Fragments arranged by size:
1017 458 360 184 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 2133
Size: 325 1808
TatI w'GTAC_w
Cuts at: 0 1256 2133
Size: 1256 877
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 899 931 1238 1270 2133
Size: 57 32 810 32 307 32 863
Fragments arranged by size:
863 810 307 57 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 47 2133
Size: 47 2086
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 467 2133
Size: 467 1666
XmaI C'CCGG_G
Cuts at: 0 803 2133
Size: 803 1330
Enzymes that do cut:
AccI AcuI AhdI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BciVI BglI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaWI BsaXI
BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI Hin4I MscI MslI MspA1I NcoI NdeI
PasI PfoI PpuMI PspOMI PstI PvuII SanDI SapI
SfcI SmaI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BclI BfrBI BlpI BmtI BplI BsaI
BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoICRI FseI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI XbaI XhoI XmnI ZraI