Restriction map for
pF1KB9981
(Linear) (Six-base) MAPSORT of: KIBB9981.seq Check: 3536 from: 1 to: 1683
KIBB9981.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 1683
Size: 597 1086
AccI GT'mk_AC
Cuts at: 0 16 440 1683
Size: 16 424 1243
Fragments arranged by size:
1243 424 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1683
Size: 1177 506
AlwNI CAG_nnn'CTG
Cuts at: 0 69 849 1305 1489 1647 1683
Size: 69 780 456 184 158 36
Fragments arranged by size:
780 456 184 158 69 36
ApaI G_GGCC'C
Cuts at: 0 641 1683
Size: 641 1042
AvaI C'yCGr_G
Cuts at: 0 29 521 706 1225 1683
Size: 29 492 185 519 458
Fragments arranged by size:
519 492 458 185 29
BamHI G'GATC_C
Cuts at: 0 1062 1212 1683
Size: 1062 150 471
Fragments arranged by size:
1062 471 150
BanI G'GyrC_C
Cuts at: 0 137 306 468 1242 1683
Size: 137 169 162 774 441
Fragments arranged by size:
774 441 169 162 137
BanII G_rGCy'C
Cuts at: 0 641 803 1683
Size: 641 162 880
Fragments arranged by size:
880 641 162
BbeI G_GCGC'C
Cuts at: 0 1246 1683
Size: 1246 437
BbvCI CC'TCA_GC
Cuts at: 0 891 1683
Size: 891 792
BglI GCCn_nnn'nGGC
Cuts at: 0 164 986 1351 1683
Size: 164 822 365 332
Fragments arranged by size:
822 365 332 164
BglII A'GATC_T
Cuts at: 0 606 1293 1683
Size: 606 687 390
Fragments arranged by size:
687 606 390
Bme1580I G_kGCm'C
Cuts at: 0 36 176 452 473 641 742 965 1415
Size: 36 140 276 21 168 101 223 450
Cuts at: 1415 1683
Size: 268
Fragments arranged by size:
450 276 268 223 168 140 101 36
21
BmgBI CAC'GTC
Cuts at: 0 14 1683
Size: 14 1669
BmrI ACTGGGnnnn_n'
Cuts at: 0 952 1540 1683
Size: 952 588 143
Fragments arranged by size:
952 588 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 459 563 631 779 827 1683
Size: 329 130 104 68 148 48 856
Fragments arranged by size:
856 329 148 130 104 68 48
Bpu10I CC'TnA_GC
Cuts at: 0 891 1175 1683
Size: 891 284 508
Fragments arranged by size:
891 508 284
BsaHI Gr'CG_yC
Cuts at: 0 1243 1683
Size: 1243 440
BsaWI w'CCGG_w
Cuts at: 0 1288 1683
Size: 1288 395
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1243 1273 1683
Size: 1243 30 410
Fragments arranged by size:
1243 410 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1150 1683
Size: 1150 533
BseYI C'CCAG_C
Cuts at: 0 20 207 220 240 245 315 407 771
Size: 20 187 13 20 5 70 92 364
Cuts at: 771 842 946 1092 1318 1683
Size: 71 104 146 226 365
Fragments arranged by size:
365 364 226 187 146 104 92 71
70 20 20 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1188 1636 1683
Size: 1188 448 47
Fragments arranged by size:
1188 448 47
BsiHKAI G_wGCw'C
Cuts at: 0 1506 1683
Size: 1506 177
BsmI GAATG_Cn'
Cuts at: 0 1121 1683
Size: 1121 562
Bsp1286I G_dGCh'C
Cuts at: 0 36 176 452 473 641 742 803 965
Size: 36 140 276 21 168 101 61 162
Cuts at: 965 1415 1506 1683
Size: 450 91 177
Fragments arranged by size:
450 276 177 168 162 140 101 91
61 36 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 597 1510 1683
Size: 597 913 173
Fragments arranged by size:
913 597 173
BsrDI GCAATG_nn'
Cuts at: 0 5 288 1330 1540 1683
Size: 5 283 1042 210 143
Fragments arranged by size:
1042 283 210 143 5
BsrFI r'CCGG_y
Cuts at: 0 1001 1239 1475 1683
Size: 1001 238 236 208
Fragments arranged by size:
1001 238 236 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1489 1647 1683
Size: 1489 158 36
Fragments arranged by size:
1489 158 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1557 1683
Size: 1557 126
BstYI r'GATC_y
Cuts at: 0 606 752 1062 1180 1212 1293 1683
Size: 606 146 310 118 32 81 390
Fragments arranged by size:
606 390 310 146 118 81 32
Bsu36I CC'TnA_GG
Cuts at: 0 336 938 1683
Size: 336 602 745
Fragments arranged by size:
745 602 336
BtgI C'CryG_G
Cuts at: 0 1358 1683
Size: 1358 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1429 1683
Size: 1429 254
BtsI GCAGTG_nn'
Cuts at: 0 1624 1683
Size: 1624 59
EaeI y'GGCC_r
Cuts at: 0 2 156 851 1130 1683
Size: 2 154 695 279 553
Fragments arranged by size:
695 553 279 154 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 1683
Size: 67 1616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 459 563 631 779 827 1177 1683
Size: 329 130 104 68 148 48 350 506
Fragments arranged by size:
506 350 329 148 130 104 68 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 974 1683
Size: 974 709
EcoO109I rG'GnC_Cy
Cuts at: 0 99 340 404 637 638 878 942 1045
Size: 99 241 64 233 1 240 64 103
Cuts at: 1045 1187 1254 1644 1683
Size: 142 67 390 39
Fragments arranged by size:
390 241 240 233 142 103 99 67
64 64 39 1
HaeII r_GCGC'y
Cuts at: 0 119 1246 1683
Size: 119 1127 437
Fragments arranged by size:
1127 437 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 74 106 889 921 1243 1275 1493 1525
Size: 74 32 783 32 322 32 218 32
Cuts at: 1525 1683
Size: 158
Fragments arranged by size:
783 322 218 158 74 32 32 32
32
HincII GTy'rAC
Cuts at: 0 17 1683
Size: 17 1666
KasI G'GCGC_C
Cuts at: 0 1242 1683
Size: 1242 441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 686 1683
Size: 355 331 997
Fragments arranged by size:
997 355 331
MscI TGG'CCA
Cuts at: 0 4 853 1683
Size: 4 849 830
Fragments arranged by size:
849 830 4
MslI CAynn'nnrTG
Cuts at: 0 281 1106 1126 1683
Size: 281 825 20 557
Fragments arranged by size:
825 557 281 20
MspA1I CmG'CkG
Cuts at: 0 24 52 349 411 895 1683
Size: 24 28 297 62 484 788
Fragments arranged by size:
788 484 297 62 28 24
NaeI GCC'GGC
Cuts at: 0 1241 1477 1683
Size: 1241 236 206
Fragments arranged by size:
1241 236 206
NarI GG'CG_CC
Cuts at: 0 1243 1683
Size: 1243 440
NgoMIV G'CCGG_C
Cuts at: 0 1239 1475 1683
Size: 1239 236 208
Fragments arranged by size:
1239 236 208
PasI CC'CwG_GG
Cuts at: 0 1264 1470 1578 1683
Size: 1264 206 108 105
Fragments arranged by size:
1264 206 108 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 849 1611 1683
Size: 849 762 72
Fragments arranged by size:
849 762 72
PfoI T'CCnGG_A
Cuts at: 0 995 1683
Size: 995 688
PpuMI rG'GwC_Cy
Cuts at: 0 99 340 404 1683
Size: 99 241 64 1279
Fragments arranged by size:
1279 241 99 64
PspOMI G'GGCC_C
Cuts at: 0 637 1683
Size: 637 1046
PstI C_TGCA'G
Cuts at: 0 107 594 1301 1352 1521 1683
Size: 107 487 707 51 169 162
Fragments arranged by size:
707 487 169 162 107 51
PvuII CAG'CTG
Cuts at: 0 24 349 411 895 1683
Size: 24 325 62 484 788
Fragments arranged by size:
788 484 325 62 24
SalI G'TCGA_C
Cuts at: 0 15 1683
Size: 15 1668
SanDI GG'GwC_CC
Cuts at: 0 404 1683
Size: 404 1279
SbfI CC_TGCA'GG
Cuts at: 0 1352 1683
Size: 1352 331
SfcI C'TryA_G
Cuts at: 0 103 590 1297 1348 1517 1683
Size: 103 487 707 51 169 166
Fragments arranged by size:
707 487 169 166 103 51
SfoI GGC'GCC
Cuts at: 0 1244 1683
Size: 1244 439
SmaI CCC'GGG
Cuts at: 0 31 523 1683
Size: 31 492 1160
Fragments arranged by size:
1160 492 31
SrfI GCCC'GGGC
Cuts at: 0 31 1683
Size: 31 1652
StuI AGG'CCT
Cuts at: 0 1201 1683
Size: 1201 482
StyI C'CwwG_G
Cuts at: 0 431 812 1049 1683
Size: 431 381 237 634
Fragments arranged by size:
634 431 381 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 228 1683
Size: 228 1455
TatI w'GTAC_w
Cuts at: 0 1307 1683
Size: 1307 376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 1197 1229 1683
Size: 121 32 1044 32 454
Fragments arranged by size:
1044 454 121 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1147 1683
Size: 1147 536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 611 1608 1683
Size: 429 182 997 75
Fragments arranged by size:
997 429 182 75
XmaI C'CCGG_G
Cuts at: 0 29 521 1683
Size: 29 492 1162
Fragments arranged by size:
1162 492 29
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbvCI BglI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SalI SanDI SbfI SfcI
SfoI SmaI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BciVI BclI BfrBI BlpI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI
EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SapI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI XbaI XhoI XmnI
ZraI