Restriction map for
pF1KB9950
(Linear) (Six-base) MAPSORT of: KIBB9950.seq Check: 161 from: 1 to: 2025
KIBB9950.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 353 2025
Size: 353 1672
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 875 1888 2025
Size: 532 343 1013 137
Fragments arranged by size:
1013 532 343 137
AhdI GACnn_n'nnGTC
Cuts at: 0 1206 2025
Size: 1206 819
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1076 2025
Size: 1076 949
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 1704 1736 2025
Size: 370 32 1302 32 289
Fragments arranged by size:
1302 370 289 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 532 1943 2025
Size: 532 1411 82
Fragments arranged by size:
1411 532 82
ApoI r'AATT_y
Cuts at: 0 832 966 1366 1420 2025
Size: 832 134 400 54 605
Fragments arranged by size:
832 605 400 134 54
AvaI C'yCGr_G
Cuts at: 0 202 521 2025
Size: 202 319 1504
Fragments arranged by size:
1504 319 202
BamHI G'GATC_C
Cuts at: 0 157 1796 2025
Size: 157 1639 229
Fragments arranged by size:
1639 229 157
BanII G_rGCy'C
Cuts at: 0 1351 2025
Size: 1351 674
BbsI GAAGACnn'nnnn_
Cuts at: 0 719 1982 2025
Size: 719 1263 43
Fragments arranged by size:
1263 719 43
BclI T'GATC_A
Cuts at: 0 1151 1337 2025
Size: 1151 186 688
Fragments arranged by size:
1151 688 186
BfrBI ATG'CAT
Cuts at: 0 277 2025
Size: 277 1748
BglII A'GATC_T
Cuts at: 0 1638 2025
Size: 1638 387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1724 2025
Size: 888 836 301
Fragments arranged by size:
888 836 301
BsaI GGTCTCn'nnnn_
Cuts at: 0 1878 2025
Size: 1878 147
BsaAI yAC'GTr
Cuts at: 0 1050 2025
Size: 1050 975
BsaBI GATnn'nnATC
Cuts at: 0 912 2025
Size: 912 1113
BsaHI Gr'CG_yC
Cuts at: 0 214 2025
Size: 214 1811
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 2025
Size: 592 30 1403
Fragments arranged by size:
1403 592 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 1518 2025
Size: 220 1298 507
Fragments arranged by size:
1298 507 220
BseYI C'CCAG_C
Cuts at: 0 611 677 1266 1296 1532 2025
Size: 611 66 589 30 236 493
Fragments arranged by size:
611 589 493 236 66 30
Bsp1286I G_dGCh'C
Cuts at: 0 1351 2025
Size: 1351 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 2025
Size: 353 1672
BsrDI GCAATG_nn'
Cuts at: 0 1485 2025
Size: 1485 540
BsrFI r'CCGG_y
Cuts at: 0 1895 2025
Size: 1895 130
BsrGI T'GTAC_A
Cuts at: 0 986 1934 2025
Size: 986 948 91
Fragments arranged by size:
986 948 91
BssSI C'ACGA_G
Cuts at: 0 1948 2025
Size: 1948 77
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1484 2025
Size: 1484 541
BstEII G'GTnAC_C
Cuts at: 0 334 2025
Size: 334 1691
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1730 2025
Size: 1730 295
BstYI r'GATC_y
Cuts at: 0 157 1638 1796 2025
Size: 157 1481 158 229
Fragments arranged by size:
1481 229 158 157
Bsu36I CC'TnA_GG
Cuts at: 0 1501 2025
Size: 1501 524
BtsI GCAGTG_nn'
Cuts at: 0 768 2025
Size: 768 1257
DraIII CAC_nnn'GTG
Cuts at: 0 1173 2025
Size: 1173 852
DrdI GACnn_nn'nnGTC
Cuts at: 0 1696 2025
Size: 1696 329
EaeI y'GGCC_r
Cuts at: 0 1275 2025
Size: 1275 750
EarI CTCTTCn'nnn_
Cuts at: 0 281 467 854 1238 2025
Size: 281 186 387 384 787
Fragments arranged by size:
787 387 384 281 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1153 2025
Size: 1153 872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 875 888 1724 1888 2025
Size: 532 343 13 836 164 137
Fragments arranged by size:
836 532 343 164 137 13
EcoO109I rG'GnC_Cy
Cuts at: 0 1194 2025
Size: 1194 831
EcoRI G'AATT_C
Cuts at: 0 832 1366 2025
Size: 832 534 659
Fragments arranged by size:
832 659 534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 46 103 277 2025
Size: 46 57 174 1748
Fragments arranged by size:
1748 174 57 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 282 314 416 448 538 570 574
Size: 27 255 32 102 32 90 32 4
Cuts at: 574 606 640 672 1192 1224 2025
Size: 32 34 32 520 32 801
Fragments arranged by size:
801 520 255 102 90 34 32 32
32 32 32 32 27 4
HincII GTy'rAC
Cuts at: 0 1178 2025
Size: 1178 847
HindIII A'AGCT_T
Cuts at: 0 1114 2025
Size: 1114 911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1689 2025
Size: 1689 336
MscI TGG'CCA
Cuts at: 0 1277 2025
Size: 1277 748
MslI CAynn'nnrTG
Cuts at: 0 1058 2025
Size: 1058 967
MspA1I CmG'CkG
Cuts at: 0 1296 1940 2025
Size: 1296 644 85
Fragments arranged by size:
1296 644 85
NdeI CA'TA_TG
Cuts at: 0 444 2025
Size: 444 1581
NsiI A_TGCA'T
Cuts at: 0 279 2025
Size: 279 1746
NspI r_CATG'y
Cuts at: 0 696 2025
Size: 696 1329
PasI CC'CwG_GG
Cuts at: 0 1212 1878 2025
Size: 1212 666 147
Fragments arranged by size:
1212 666 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 2025
Size: 348 1677
PmlI CAC'GTG
Cuts at: 0 1050 2025
Size: 1050 975
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 911 943 2025
Size: 17 32 862 32 1082
Fragments arranged by size:
1082 862 32 32 17
PpuMI rG'GwC_Cy
Cuts at: 0 1194 2025
Size: 1194 831
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1060 1092 2025
Size: 1060 32 933
Fragments arranged by size:
1060 933 32
PstI C_TGCA'G
Cuts at: 0 465 2025
Size: 465 1560
PvuII CAG'CTG
Cuts at: 0 1296 1940 2025
Size: 1296 644 85
Fragments arranged by size:
1296 644 85
SfcI C'TryA_G
Cuts at: 0 218 461 2025
Size: 218 243 1564
Fragments arranged by size:
1564 243 218
SmlI C'TyrA_G
Cuts at: 0 202 2025
Size: 202 1823
StyI C'CwwG_G
Cuts at: 0 706 2025
Size: 706 1319
TatI w'GTAC_w
Cuts at: 0 986 1934 2025
Size: 986 948 91
Fragments arranged by size:
986 948 91
XbaI T'CTAG_A
Cuts at: 0 18 2025
Size: 18 2007
XhoI C'TCGA_G
Cuts at: 0 202 2025
Size: 202 1823
XmnI GAAnn'nnTTC
Cuts at: 0 26 29 1370 2003 2025
Size: 26 3 1341 633 22
Fragments arranged by size:
1341 633 26 22 3
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI AlwNI ApoI AvaI
BamHI BanII BbsI BclI BfrBI BglII BpmI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI Bsp1286I BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtsI DraIII DrdI EaeI EarI EciI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NdeI NsiI NspI PasI PflMI PmlI
PpiI PpuMI PsrI PstI PvuII SfcI SmlI StyI
TatI XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaWI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BssHII BstBI BstZ17I BtgI BtgZI ClaI CspCI DraI
EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PciI PfoI PmeI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XcmI XmaI ZraI