Restriction map for
pF1KB9943
(Linear) (Six-base) MAPSORT of: KIBB9943.seq Check: 4694 from: 1 to: 1491
KIBB9943.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 662 1491
Size: 662 829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 148 279 397 703 1491
Size: 122 26 131 118 306 788
Fragments arranged by size:
788 306 131 122 118 26
AleI CACnn'nnGTG
Cuts at: 0 997 1491
Size: 997 494
AlwNI CAG_nnn'CTG
Cuts at: 0 1423 1491
Size: 1423 68
ApoI r'AATT_y
Cuts at: 0 146 205 226 712 962 1237 1310 1491
Size: 146 59 21 486 250 275 73 181
Fragments arranged by size:
486 275 250 181 146 73 59 21
BanI G'GyrC_C
Cuts at: 0 742 1491
Size: 742 749
BbsI GAAGACnn'nnnn_
Cuts at: 0 559 1491
Size: 559 932
BclI T'GATC_A
Cuts at: 0 759 1491
Size: 759 732
BmrI ACTGGGnnnn_n'
Cuts at: 0 1119 1491
Size: 1119 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1485 1491
Size: 1485 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 1491
Size: 630 861
BsaBI GATnn'nnATC
Cuts at: 0 758 1491
Size: 758 733
BsaHI Gr'CG_yC
Cuts at: 0 100 1491
Size: 100 1391
BseYI C'CCAG_C
Cuts at: 0 1214 1491
Size: 1214 277
BsrFI r'CCGG_y
Cuts at: 0 85 1491
Size: 85 1406
BstAPI GCAn_nnn'nTGC
Cuts at: 0 312 1491
Size: 312 1179
BstYI r'GATC_y
Cuts at: 0 1353 1491
Size: 1353 138
BtgI C'CryG_G
Cuts at: 0 41 1491
Size: 41 1450
DraI TTT'AAA
Cuts at: 0 231 507 1491
Size: 231 276 984
Fragments arranged by size:
984 276 231
EaeI y'GGCC_r
Cuts at: 0 2 35 74 383 1491
Size: 2 33 39 309 1108
Fragments arranged by size:
1108 309 39 33 2
EarI CTCTTCn'nnn_
Cuts at: 0 506 527 542 686 689 1136 1479 1491
Size: 506 21 15 144 3 447 343 12
Fragments arranged by size:
506 447 343 144 21 15 12 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 148 279 397 703 1485 1491
Size: 122 26 131 118 306 782 6
Fragments arranged by size:
782 306 131 122 118 26 6
EcoRI G'AATT_C
Cuts at: 0 146 1491
Size: 146 1345
HaeII r_GCGC'y
Cuts at: 0 109 1179 1491
Size: 109 1070 312
Fragments arranged by size:
1070 312 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 1491
Size: 353 32 1106
Fragments arranged by size:
1106 353 32
HindIII A'AGCT_T
Cuts at: 0 629 1076 1491
Size: 629 447 415
Fragments arranged by size:
629 447 415
MfeI C'AATT_G
Cuts at: 0 379 1491
Size: 379 1112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1483 1491
Size: 1483 8
MscI TGG'CCA
Cuts at: 0 385 1491
Size: 385 1106
MslI CAynn'nnrTG
Cuts at: 0 398 997 1491
Size: 398 599 494
Fragments arranged by size:
599 494 398
MspA1I CmG'CkG
Cuts at: 0 43 1491
Size: 43 1448
PflMI CCAn_nnn'nTGG
Cuts at: 0 1385 1491
Size: 1385 106
PfoI T'CCnGG_A
Cuts at: 0 837 1491
Size: 837 654
SacII CC_GC'GG
Cuts at: 0 44 1491
Size: 44 1447
SapI GCTCTTCn'nnn_
Cuts at: 0 506 527 1491
Size: 506 21 964
Fragments arranged by size:
964 506 21
ScaI AGT'ACT
Cuts at: 0 135 1491
Size: 135 1356
SfcI C'TryA_G
Cuts at: 0 659 1491
Size: 659 832
SmlI C'TyrA_G
Cuts at: 0 645 1491
Size: 645 846
TatI w'GTAC_w
Cuts at: 0 133 1491
Size: 133 1358
XbaI T'CTAG_A
Cuts at: 0 825 897 1491
Size: 825 72 594
Fragments arranged by size:
825 594 72
XmnI GAAnn'nnTTC
Cuts at: 0 631 821 833 1491
Size: 631 190 12 658
Fragments arranged by size:
658 631 190 12
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI BanI BbsI BclI
BmrI BpmI BpuEI BsaBI BsaHI BseYI BsrFI BstAPI
BstYI BtgI DraI EaeI EarI Eco57MI EcoRI HaeII
Hin4I HindIII MfeI MmeI MscI MslI MspA1I PflMI
PfoI SacII SapI ScaI SfcI SmlI TatI XbaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XcmI
XhoI XmaI ZraI