Restriction map for
pF1KB9941
(Linear) (Six-base) MAPSORT of: KIBB9941.seq Check: 6396 from: 1 to: 1587
KIBB9941.orf
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 350 443 898 1587
Size: 57 293 93 455 689
Fragments arranged by size:
689 455 293 93 57
AflIII A'CryG_T
Cuts at: 0 1319 1587
Size: 1319 268
AhdI GACnn_n'nnGTC
Cuts at: 0 1323 1484 1587
Size: 1323 161 103
Fragments arranged by size:
1323 161 103
AleI CACnn'nnGTG
Cuts at: 0 859 1587
Size: 859 728
AlwNI CAG_nnn'CTG
Cuts at: 0 663 744 1285 1587
Size: 663 81 541 302
Fragments arranged by size:
663 541 302 81
AvaI C'yCGr_G
Cuts at: 0 1573 1587
Size: 1573 14
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1549 1582 1587
Size: 1549 33 5
Fragments arranged by size:
1549 33 5
BamHI G'GATC_C
Cuts at: 0 1334 1587
Size: 1334 253
BanI G'GyrC_C
Cuts at: 0 205 532 808 886 1587
Size: 205 327 276 78 701
Fragments arranged by size:
701 327 276 205 78
BanII G_rGCy'C
Cuts at: 0 986 1352 1587
Size: 986 366 235
Fragments arranged by size:
986 366 235
BbsI GAAGACnn'nnnn_
Cuts at: 0 877 1587
Size: 877 710
BbvCI CC'TCA_GC
Cuts at: 0 1100 1587
Size: 1100 487
BglI GCCn_nnn'nGGC
Cuts at: 0 806 1442 1587
Size: 806 636 145
Fragments arranged by size:
806 636 145
Bme1580I G_kGCm'C
Cuts at: 0 811 841 889 1438 1587
Size: 811 30 48 549 149
Fragments arranged by size:
811 549 149 48 30
BmgBI CAC'GTC
Cuts at: 0 1320 1587
Size: 1320 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 567 1587
Size: 567 1020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 931 1424 1555 1587
Size: 814 117 493 131 32
Fragments arranged by size:
814 493 131 117 32
Bpu10I CC'TnA_GC
Cuts at: 0 1100 1587
Size: 1100 487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1587
Size: 1021 566
BsaI GGTCTCn'nnnn_
Cuts at: 0 359 514 1587
Size: 359 155 1073
Fragments arranged by size:
1073 359 155
BsaAI yAC'GTr
Cuts at: 0 396 842 1587
Size: 396 446 745
Fragments arranged by size:
745 446 396
BsaHI Gr'CG_yC
Cuts at: 0 761 1485 1587
Size: 761 724 102
Fragments arranged by size:
761 724 102
BsaWI w'CCGG_w
Cuts at: 0 364 1587
Size: 364 1223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 1534 1564 1587
Size: 337 30 1167 30 23
Fragments arranged by size:
1167 337 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 928 994 1513 1587
Size: 159 769 66 519 74
Fragments arranged by size:
769 519 159 74 66
BseYI C'CCAG_C
Cuts at: 0 385 744 804 834 1449 1587
Size: 385 359 60 30 615 138
Fragments arranged by size:
615 385 359 138 60 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 688 705 976 1587
Size: 676 12 17 271 611
Fragments arranged by size:
676 611 271 17 12
BsiHKAI G_wGCw'C
Cuts at: 0 1352 1587
Size: 1352 235
BsmI GAATG_Cn'
Cuts at: 0 380 1587
Size: 380 1207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1219 1587
Size: 1219 368
Bsp1286I G_dGCh'C
Cuts at: 0 811 841 889 986 1352 1438 1587
Size: 811 30 48 97 366 86 149
Fragments arranged by size:
811 366 149 97 86 48 30
BspEI T'CCGG_A
Cuts at: 0 364 1587
Size: 364 1223
BspHI T'CATG_A
Cuts at: 0 728 926 1587
Size: 728 198 661
Fragments arranged by size:
728 661 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 713 912 1529 1587
Size: 713 199 617 58
Fragments arranged by size:
713 617 199 58
BsrBI CCG'CTC
Cuts at: 0 1176 1587
Size: 1176 411
BsrDI GCAATG_nn'
Cuts at: 0 245 1587
Size: 245 1342
BsrFI r'CCGG_y
Cuts at: 0 30 800 811 1587
Size: 30 770 11 776
Fragments arranged by size:
776 770 30 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 815 1587
Size: 815 772
BstEII G'GTnAC_C
Cuts at: 0 234 1587
Size: 234 1353
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 1164 1587
Size: 792 372 423
Fragments arranged by size:
792 423 372
BstYI r'GATC_y
Cuts at: 0 596 1334 1587
Size: 596 738 253
Fragments arranged by size:
738 596 253
Bsu36I CC'TnA_GG
Cuts at: 0 1147 1587
Size: 1147 440
BtgI C'CryG_G
Cuts at: 0 38 968 1469 1587
Size: 38 930 501 118
Fragments arranged by size:
930 501 118 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 696 1587
Size: 696 891
EaeI y'GGCC_r
Cuts at: 0 35 41 752 1587
Size: 35 6 711 835
Fragments arranged by size:
835 711 35 6
EarI CTCTTCn'nnn_
Cuts at: 0 419 1587
Size: 419 1168
EcoICRI GAG'CTC
Cuts at: 0 1350 1587
Size: 1350 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 350 443 814 898 931 1424 1555
Size: 57 293 93 371 84 33 493 131
Cuts at: 1555 1587
Size: 32
Fragments arranged by size:
493 371 293 131 93 84 57 33
32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 754 913 1462 1587
Size: 754 159 549 125
Fragments arranged by size:
754 549 159 125
EcoO109I rG'GnC_Cy
Cuts at: 0 497 1587
Size: 497 1090
HaeII r_GCGC'y
Cuts at: 0 801 1587
Size: 801 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 1292 1309 1324 1341 1470 1502
Size: 337 32 923 17 15 17 129 32
Cuts at: 1502 1587
Size: 85
Fragments arranged by size:
923 337 129 85 32 32 17 17
15
HincII GTy'rAC
Cuts at: 0 408 1587
Size: 408 1179
MfeI C'AATT_G
Cuts at: 0 1072 1587
Size: 1072 515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1382 1587
Size: 956 426 205
Fragments arranged by size:
956 426 205
MscI TGG'CCA
Cuts at: 0 37 754 1587
Size: 37 717 833
Fragments arranged by size:
833 717 37
MslI CAynn'nnrTG
Cuts at: 0 682 727 859 1587
Size: 682 45 132 728
Fragments arranged by size:
728 682 132 45
MspA1I CmG'CkG
Cuts at: 0 471 960 1555 1587
Size: 471 489 595 32
Fragments arranged by size:
595 489 471 32
NaeI GCC'GGC
Cuts at: 0 802 1587
Size: 802 785
NcoI C'CATG_G
Cuts at: 0 1469 1587
Size: 1469 118
NgoMIV G'CCGG_C
Cuts at: 0 800 1587
Size: 800 787
NspI r_CATG'y
Cuts at: 0 1431 1587
Size: 1431 156
PasI CC'CwG_GG
Cuts at: 0 492 1587
Size: 492 1095
PflMI CCAn_nnn'nTGG
Cuts at: 0 1077 1268 1587
Size: 1077 191 319
Fragments arranged by size:
1077 319 191
PfoI T'CCnGG_A
Cuts at: 0 551 908 1532 1587
Size: 551 357 624 55
Fragments arranged by size:
624 551 357 55
PmlI CAC'GTG
Cuts at: 0 842 1587
Size: 842 745
PstI C_TGCA'G
Cuts at: 0 1526 1587
Size: 1526 61
PvuII CAG'CTG
Cuts at: 0 471 960 1587
Size: 471 489 627
Fragments arranged by size:
627 489 471
SacI G_AGCT'C
Cuts at: 0 1352 1587
Size: 1352 235
SfcI C'TryA_G
Cuts at: 0 156 1522 1587
Size: 156 1366 65
Fragments arranged by size:
1366 156 65
SmaI CCC'GGG
Cuts at: 0 1575 1587
Size: 1575 12
SmlI C'TyrA_G
Cuts at: 0 1036 1587
Size: 1036 551
StuI AGG'CCT
Cuts at: 0 904 1506 1587
Size: 904 602 81
Fragments arranged by size:
904 602 81
StyI C'CwwG_G
Cuts at: 0 44 296 755 1245 1358 1469 1587
Size: 44 252 459 490 113 111 118
Fragments arranged by size:
490 459 252 118 113 111 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 341 1015 1587
Size: 341 674 572
Fragments arranged by size:
674 572 341
TatI w'GTAC_w
Cuts at: 0 1232 1587
Size: 1232 355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 1587
Size: 393 1194
XmaI C'CCGG_G
Cuts at: 0 1573 1587
Size: 1573 14
XmnI GAAnn'nnTTC
Cuts at: 0 904 1587
Size: 904 683
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PstI PvuII SacI SfcI SmaI SmlI
StuI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI BsaBI BsiEI BsiWI BsrGI BssHII BssSI
BstBI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI ZraI