Restriction map for
pF1KB9923
(Linear) (Six-base) MAPSORT of: KIBB9923.seq Check: 7342 from: 1 to: 1026
KIBB9923.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 997 1026
Size: 892 105 29
Fragments arranged by size:
892 105 29
AleI CACnn'nnGTG
Cuts at: 0 688 1026
Size: 688 338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 347 1026
Size: 347 679
AlwNI CAG_nnn'CTG
Cuts at: 0 837 1026
Size: 837 189
ApaI G_GGCC'C
Cuts at: 0 246 791 1026
Size: 246 545 235
Fragments arranged by size:
545 246 235
ApaLI G'TGCA_C
Cuts at: 0 322 895 1026
Size: 322 573 131
Fragments arranged by size:
573 322 131
AvaI C'yCGr_G
Cuts at: 0 217 239 499 608 1026
Size: 217 22 260 109 418
Fragments arranged by size:
418 260 217 109 22
BanI G'GyrC_C
Cuts at: 0 513 1026
Size: 513 513
BanII G_rGCy'C
Cuts at: 0 246 791 824 1026
Size: 246 545 33 202
Fragments arranged by size:
545 246 202 33
BglI GCCn_nnn'nGGC
Cuts at: 0 550 756 1026
Size: 550 206 270
Fragments arranged by size:
550 270 206
BlpI GC'TnA_GC
Cuts at: 0 629 695 1026
Size: 629 66 331
Fragments arranged by size:
629 331 66
Bme1580I G_kGCm'C
Cuts at: 0 246 326 791 899 1026
Size: 246 80 465 108 127
Fragments arranged by size:
465 246 127 108 80
BmgBI CAC'GTC
Cuts at: 0 900 1026
Size: 900 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 914 1026
Size: 914 112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 634 1026
Size: 634 392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1026
Size: 859 167
Bpu10I CC'TnA_GC
Cuts at: 0 805 1026
Size: 805 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1026
Size: 340 686
BsaI GGTCTCn'nnnn_
Cuts at: 0 173 1026
Size: 173 853
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 703 733 1026
Size: 637 30 36 30 293
Fragments arranged by size:
637 293 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 295 553 601 778 1003 1026
Size: 235 60 258 48 177 225 23
Fragments arranged by size:
258 235 225 177 60 48 23
BseYI C'CCAG_C
Cuts at: 0 350 676 1026
Size: 350 326 350
Fragments arranged by size:
350 350 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 988 1026
Size: 854 134 38
Fragments arranged by size:
854 134 38
BsiHKAI G_wGCw'C
Cuts at: 0 326 583 824 899 1026
Size: 326 257 241 75 127
Fragments arranged by size:
326 257 241 127 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 881 1026
Size: 881 145
Bsp1286I G_dGCh'C
Cuts at: 0 246 326 583 791 824 899 1026
Size: 246 80 257 208 33 75 127
Fragments arranged by size:
257 246 208 127 80 75 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 339 1026
Size: 314 25 687
Fragments arranged by size:
687 314 25
BsrBI CCG'CTC
Cuts at: 0 285 1026
Size: 285 741
BsrFI r'CCGG_y
Cuts at: 0 257 1026
Size: 257 769
BstAPI GCAn_nnn'nTGC
Cuts at: 0 903 1026
Size: 903 123
BstEII G'GTnAC_C
Cuts at: 0 381 1026
Size: 381 645
BstXI CCAn_nnnn'nTGG
Cuts at: 0 253 690 1026
Size: 253 437 336
Fragments arranged by size:
437 336 253
BstYI r'GATC_y
Cuts at: 0 558 992 1026
Size: 558 434 34
Fragments arranged by size:
558 434 34
EaeI y'GGCC_r
Cuts at: 0 255 1026
Size: 255 771
EarI CTCTTCn'nnn_
Cuts at: 0 211 646 764 1026
Size: 211 435 118 262
Fragments arranged by size:
435 262 211 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 1026
Size: 199 827
EcoICRI GAG'CTC
Cuts at: 0 822 1026
Size: 822 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 892 997 1026
Size: 859 33 105 29
Fragments arranged by size:
859 105 33 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 608 622 808 1026
Size: 608 14 186 218
Fragments arranged by size:
608 218 186 14
EcoO109I rG'GnC_Cy
Cuts at: 0 114 303 408 495 552 613 787 834
Size: 114 189 105 87 57 61 174 47
Cuts at: 834 1026
Size: 192
Fragments arranged by size:
192 189 174 114 105 87 61 57
47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 39 381 1026
Size: 39 342 645
Fragments arranged by size:
645 342 39
HaeII r_GCGC'y
Cuts at: 0 860 1026
Size: 860 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 637 669 703 735 1026
Size: 577 32 28 32 34 32 291
Fragments arranged by size:
577 291 34 32 32 32 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1026
Size: 26 1000
MslI CAynn'nnrTG
Cuts at: 0 688 1026
Size: 688 338
MspA1I CmG'CkG
Cuts at: 0 967 1026
Size: 967 59
PasI CC'CwG_GG
Cuts at: 0 743 1026
Size: 743 283
PpuMI rG'GwC_Cy
Cuts at: 0 114 303 408 1026
Size: 114 189 105 618
Fragments arranged by size:
618 189 114 105
PspOMI G'GGCC_C
Cuts at: 0 242 787 1026
Size: 242 545 239
Fragments arranged by size:
545 242 239
PspXI vC'TCGA_Gb
Cuts at: 0 217 499 1026
Size: 217 282 527
Fragments arranged by size:
527 282 217
PstI C_TGCA'G
Cuts at: 0 486 1026
Size: 486 540
PvuII CAG'CTG
Cuts at: 0 967 1026
Size: 967 59
SacI G_AGCT'C
Cuts at: 0 824 1026
Size: 824 202
SfcI C'TryA_G
Cuts at: 0 482 1026
Size: 482 544
SmaI CCC'GGG
Cuts at: 0 241 1026
Size: 241 785
SmlI C'TyrA_G
Cuts at: 0 217 355 499 1026
Size: 217 138 144 527
Fragments arranged by size:
527 217 144 138
StyI C'CwwG_G
Cuts at: 0 65 392 656 1026
Size: 65 327 264 370
Fragments arranged by size:
370 327 264 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 700 844 1026
Size: 208 492 144 182
Fragments arranged by size:
492 208 182 144
TatI w'GTAC_w
Cuts at: 0 80 1026
Size: 80 946
XhoI C'TCGA_G
Cuts at: 0 217 499 1026
Size: 217 282 527
Fragments arranged by size:
527 282 217
XmaI C'CCGG_G
Cuts at: 0 239 1026
Size: 239 787
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI
BstYI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I MmeI MslI MspA1I PasI PpuMI
PspOMI PspXI PstI PvuII SacI SfcI SmaI SmlI
StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmtI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XmnI ZraI