Restriction map for
pF1KB9898
(Linear) (Six-base) MAPSORT of: KIBB9898.seq Check: 65 from: 1 to: 6006
KIBB9898.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3045 6006
Size: 3045 2961
AccI GT'mk_AC
Cuts at: 0 5013 6006
Size: 5013 993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 520 1241 1642 1753 2105 2663 3115
Size: 304 216 721 401 111 352 558 452
Cuts at: 3115 3245 4105 4739 5861 6006
Size: 130 860 634 1122 145
Fragments arranged by size:
1122 860 721 634 558 452 401 352
304 216 145 130 111
AflIII A'CryG_T
Cuts at: 0 1401 6006
Size: 1401 4605
AhdI GACnn_n'nnGTC
Cuts at: 0 4998 6006
Size: 4998 1008
AleI CACnn'nnGTG
Cuts at: 0 3659 4068 6006
Size: 3659 409 1938
Fragments arranged by size:
3659 1938 409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1093 4086 6006
Size: 1093 2993 1920
Fragments arranged by size:
2993 1920 1093
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4777 4809 6006
Size: 4777 32 1197
Fragments arranged by size:
4777 1197 32
AlwNI CAG_nnn'CTG
Cuts at: 0 543 640 2025 2128 3093 3825 4338 4666
Size: 543 97 1385 103 965 732 513 328
Cuts at: 4666 6006
Size: 1340
Fragments arranged by size:
1385 1340 965 732 543 513 328 103
97
ApaLI G'TGCA_C
Cuts at: 0 1978 6006
Size: 1978 4028
ApoI r'AATT_y
Cuts at: 0 1051 1821 2445 3351 4213 4827 6006
Size: 1051 770 624 906 862 614 1179
Fragments arranged by size:
1179 1051 906 862 770 624 614
AvaI C'yCGr_G
Cuts at: 0 5975 6006
Size: 5975 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1882 1915 3353 3386 6006
Size: 1882 33 1438 33 2620
Fragments arranged by size:
2620 1882 1438 33 33
BamHI G'GATC_C
Cuts at: 0 3828 4690 5565 6006
Size: 3828 862 875 441
Fragments arranged by size:
3828 875 862 441
BanI G'GyrC_C
Cuts at: 0 1368 3844 3999 4324 6006
Size: 1368 2476 155 325 1682
Fragments arranged by size:
2476 1682 1368 325 155
BanII G_rGCy'C
Cuts at: 0 132 317 1844 5885 6006
Size: 132 185 1527 4041 121
Fragments arranged by size:
4041 1527 185 132 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 4208 6006
Size: 4208 1798
BbvCI CC'TCA_GC
Cuts at: 0 1247 3754 6006
Size: 1247 2507 2252
Fragments arranged by size:
2507 2252 1247
BciVI GTATCCnnnnn_n'
Cuts at: 0 3354 4991 6006
Size: 3354 1637 1015
Fragments arranged by size:
3354 1637 1015
BclI T'GATC_A
Cuts at: 0 2301 6006
Size: 2301 3705
BfrBI ATG'CAT
Cuts at: 0 4030 6006
Size: 4030 1976
BglI GCCn_nnn'nGGC
Cuts at: 0 1839 5761 6006
Size: 1839 3922 245
Fragments arranged by size:
3922 1839 245
BglII A'GATC_T
Cuts at: 0 1650 2628 6006
Size: 1650 978 3378
Fragments arranged by size:
3378 1650 978
BlpI GC'TnA_GC
Cuts at: 0 2744 5861 6006
Size: 2744 3117 145
Fragments arranged by size:
3117 2744 145
Bme1580I G_kGCm'C
Cuts at: 0 1661 1982 6006
Size: 1661 321 4024
Fragments arranged by size:
4024 1661 321
BmgBI CAC'GTC
Cuts at: 0 4275 6006
Size: 4275 1731
BmrI ACTGGGnnnn_n'
Cuts at: 0 2083 4947 6006
Size: 2083 2864 1059
Fragments arranged by size:
2864 2083 1059
BmtI G_CTAG'C
Cuts at: 0 1039 6006
Size: 1039 4967
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4470 6006
Size: 4470 1536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1900 1936 2254 2437 3985 4465 6006
Size: 85 1815 36 318 183 1548 480 1541
Fragments arranged by size:
1815 1548 1541 480 318 183 85 36
Bpu10I CC'TnA_GC
Cuts at: 0 1247 3754 4301 6006
Size: 1247 2507 547 1705
Fragments arranged by size:
2507 1705 1247 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 4790 6006
Size: 1519 3271 1216
Fragments arranged by size:
3271 1519 1216
BsaI GGTCTCn'nnnn_
Cuts at: 0 3070 4620 6006
Size: 3070 1550 1386
Fragments arranged by size:
3070 1550 1386
BsaAI yAC'GTr
Cuts at: 0 4492 6006
Size: 4492 1514
BsaBI GATnn'nnATC
Cuts at: 0 3685 6006
Size: 3685 2321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1873 1903 2650 2680 4909 4939 5573 5603
Size: 1873 30 747 30 2229 30 634 30
Cuts at: 5603 5943 5973 6006
Size: 340 30 33
Fragments arranged by size:
2229 1873 747 634 340 33 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1217 1220 4321 6006
Size: 1217 3 3101 1685
Fragments arranged by size:
3101 1685 1217 3
BseYI C'CCAG_C
Cuts at: 0 3234 4574 4583 4613 4631 6006
Size: 3234 1340 9 30 18 1375
Fragments arranged by size:
3234 1375 1340 30 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 2038 3076 5321 5537 6006
Size: 745 1293 1038 2245 216 469
Fragments arranged by size:
2245 1293 1038 745 469 216
BsiHKAI G_wGCw'C
Cuts at: 0 132 1886 1982 4065 4137 6006
Size: 132 1754 96 2083 72 1869
Fragments arranged by size:
2083 1869 1754 132 96 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3742 4704 6006
Size: 3742 962 1302
Fragments arranged by size:
3742 1302 962
Bsp1286I G_dGCh'C
Cuts at: 0 132 317 1661 1844 1886 1982 4065 4137
Size: 132 185 1344 183 42 96 2083 72
Cuts at: 4137 5885 6006
Size: 1748 121
Fragments arranged by size:
2083 1748 1344 185 183 132 121 96
72 42
BspHI T'CATG_A
Cuts at: 0 5178 6006
Size: 5178 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3045 3448 4804 5900 6006
Size: 3045 403 1356 1096 106
Fragments arranged by size:
3045 1356 1096 403 106
BsrDI GCAATG_nn'
Cuts at: 0 716 932 4905 6006
Size: 716 216 3973 1101
Fragments arranged by size:
3973 1101 716 216
BsrFI r'CCGG_y
Cuts at: 0 3782 6006
Size: 3782 2224
BsrGI T'GTAC_A
Cuts at: 0 5142 6006
Size: 5142 864
BssSI C'ACGA_G
Cuts at: 0 1646 6006
Size: 1646 4360
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2592 4027 4161 6006
Size: 2592 1435 134 1845
Fragments arranged by size:
2592 1845 1435 134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3996 6006
Size: 3996 2010
BstYI r'GATC_y
Cuts at: 0 1650 2628 3828 3911 4690 4968 5565 5687
Size: 1650 978 1200 83 779 278 597 122
Cuts at: 5687 6006
Size: 319
Fragments arranged by size:
1650 1200 978 779 597 319 278 122
83
Bsu36I CC'TnA_GG
Cuts at: 0 810 1364 4229 6006
Size: 810 554 2865 1777
Fragments arranged by size:
2865 1777 810 554
BtgI C'CryG_G
Cuts at: 0 650 3080 5588 5832 6006
Size: 650 2430 2508 244 174
Fragments arranged by size:
2508 2430 650 244 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 785 6006
Size: 785 5221
BtsI GCAGTG_nn'
Cuts at: 0 115 519 617 875 1569 1753 3206 3618
Size: 115 404 98 258 694 184 1453 412
Cuts at: 3618 3677 4112 6006
Size: 59 435 1894
Fragments arranged by size:
1894 1453 694 435 412 404 258 184
115 98 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2927 2962 6006
Size: 2927 35 3044
Fragments arranged by size:
3044 2927 35
DraI TTT'AAA
Cuts at: 0 1112 5657 6006
Size: 1112 4545 349
Fragments arranged by size:
4545 1112 349
DraIII CAC_nnn'GTG
Cuts at: 0 158 667 1977 2029 6006
Size: 158 509 1310 52 3977
Fragments arranged by size:
3977 1310 509 158 52
EaeI y'GGCC_r
Cuts at: 0 2328 3651 5190 5585 6006
Size: 2328 1323 1539 395 421
Fragments arranged by size:
2328 1539 1323 421 395
EarI CTCTTCn'nnn_
Cuts at: 0 651 1238 3611 3794 3950 4749 5333 5783
Size: 651 587 2373 183 156 799 584 450
Cuts at: 5783 6006
Size: 223
Fragments arranged by size:
2373 799 651 587 584 450 223 183
156
EcoICRI GAG'CTC
Cuts at: 0 130 6006
Size: 130 5876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 304 520 1241 1642 1753 1900 1936
Size: 85 219 216 721 401 111 147 36
Cuts at: 1936 2105 2254 2437 2663 3115 3245 3985 4105
Size: 169 149 183 226 452 130 740 120
Cuts at: 4105 4465 4739 5861 6006
Size: 360 274 1122 145
Fragments arranged by size:
1122 740 721 452 401 360 274 226
219 216 183 169 149 147 145 130
120 111 85 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1259 6006
Size: 1259 4747
EcoO109I rG'GnC_Cy
Cuts at: 0 1704 3433 3460 5986 6006
Size: 1704 1729 27 2526 20
Fragments arranged by size:
2526 1729 1704 27 20
EcoRI G'AATT_C
Cuts at: 0 1051 4827 6006
Size: 1051 3776 1179
Fragments arranged by size:
3776 1179 1051
EcoRV GAT'ATC
Cuts at: 0 4761 6006
Size: 4761 1245
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1188 1871 2650 6006
Size: 1188 683 779 3356
Fragments arranged by size:
3356 1188 779 683
FspI TGC'GCA
Cuts at: 0 2735 6006
Size: 2735 3271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 117 149 1374 1406 1873 1905
Size: 69 32 16 32 1225 32 467 32
Cuts at: 1905 2193 2225 2265 2297 2532 2564 2650 2682
Size: 288 32 40 32 235 32 86 32
Cuts at: 2682 3376 3408 4909 4941 4984 5016 5524 5556
Size: 694 32 1501 32 43 32 508 32
Cuts at: 5556 6006
Size: 450
Fragments arranged by size:
1501 1225 694 508 467 450 288 235
86 69 43 40 32 32 32 32
32 32 32 32 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 412 2725 4671 6006
Size: 412 2313 1946 1335
Fragments arranged by size:
2313 1946 1335 412
HindIII A'AGCT_T
Cuts at: 0 1853 2675 4432 6006
Size: 1853 822 1757 1574
Fragments arranged by size:
1853 1757 1574 822
MfeI C'AATT_G
Cuts at: 0 1207 6006
Size: 1207 4799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1850 5620 6006
Size: 1850 3770 386
Fragments arranged by size:
3770 1850 386
MscI TGG'CCA
Cuts at: 0 2330 3653 5192 6006
Size: 2330 1323 1539 814
Fragments arranged by size:
2330 1539 1323 814
MslI CAynn'nnrTG
Cuts at: 0 662 721 746 1404 2768 2774 3659 3691
Size: 662 59 25 658 1364 6 885 32
Cuts at: 3691 3994 4068 4182 4893 5929 6006
Size: 303 74 114 711 1036 77
Fragments arranged by size:
1364 1036 885 711 662 658 303 114
77 74 59 32 25 6
MspA1I CmG'CkG
Cuts at: 0 865 1878 3289 3711 4426 6006
Size: 865 1013 1411 422 715 1580
Fragments arranged by size:
1580 1411 1013 865 715 422
NcoI C'CATG_G
Cuts at: 0 3080 5832 6006
Size: 3080 2752 174
Fragments arranged by size:
3080 2752 174
NdeI CA'TA_TG
Cuts at: 0 5964 6006
Size: 5964 42
NheI G'CTAG_C
Cuts at: 0 1035 6006
Size: 1035 4971
NsiI A_TGCA'T
Cuts at: 0 4032 6006
Size: 4032 1974
NspI r_CATG'y
Cuts at: 0 1405 1833 2483 2595 2848 2971 3879 4121
Size: 1405 428 650 112 253 123 908 242
Cuts at: 4121 4164 5316 5367 5644 6006
Size: 43 1152 51 277 362
Fragments arranged by size:
1405 1152 908 650 428 362 277 253
242 123 112 51 43
PasI CC'CwG_GG
Cuts at: 0 4855 6006
Size: 4855 1151
PciI A'CATG_T
Cuts at: 0 1401 6006
Size: 1401 4605
PflMI CCAn_nnn'nTGG
Cuts at: 0 1555 5047 5199 5505 5602 5734 6006
Size: 1555 3492 152 306 97 132 272
Fragments arranged by size:
3492 1555 306 272 152 132 97
PfoI T'CCnGG_A
Cuts at: 0 674 6006
Size: 674 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 290 322 6006
Size: 290 32 5684
Fragments arranged by size:
5684 290 32
PpuMI rG'GwC_Cy
Cuts at: 0 3433 3460 6006
Size: 3433 27 2546
Fragments arranged by size:
3433 2546 27
PshAI GACnn'nnGTC
Cuts at: 0 1521 6006
Size: 1521 4485
PstI C_TGCA'G
Cuts at: 0 514 870 1008 1479 3216 3672 4425 4815
Size: 514 356 138 471 1737 456 753 390
Cuts at: 4815 6006
Size: 1191
Fragments arranged by size:
1737 1191 753 514 471 456 390 356
138
PvuII CAG'CTG
Cuts at: 0 865 1878 3289 4426 6006
Size: 865 1013 1411 1137 1580
Fragments arranged by size:
1580 1411 1137 1013 865
SacI G_AGCT'C
Cuts at: 0 132 6006
Size: 132 5874
SexAI A'CCwGG_T
Cuts at: 0 1973 6006
Size: 1973 4033
SfcI C'TryA_G
Cuts at: 0 458 510 632 866 1004 1475 1573 2686
Size: 458 52 122 234 138 471 98 1113
Cuts at: 2686 3212 3668 4421 4591 4811 5295 6006
Size: 526 456 753 170 220 484 711
Fragments arranged by size:
1113 753 711 526 484 471 458 456
234 220 170 138 122 98 52
SgrAI Cr'CCGG_yG
Cuts at: 0 3782 6006
Size: 3782 2224
SmlI C'TyrA_G
Cuts at: 0 1534 4805 6006
Size: 1534 3271 1201
Fragments arranged by size:
3271 1534 1201
SpeI A'CTAG_T
Cuts at: 0 5493 6006
Size: 5493 513
SphI G_CATG'C
Cuts at: 0 2483 2848 5644 6006
Size: 2483 365 2796 362
Fragments arranged by size:
2796 2483 365 362
SspI AAT'ATT
Cuts at: 0 697 2599 6006
Size: 697 1902 3407
Fragments arranged by size:
3407 1902 697
StuI AGG'CCT
Cuts at: 0 883 1437 6006
Size: 883 554 4569
Fragments arranged by size:
4569 883 554
StyI C'CwwG_G
Cuts at: 0 1846 1929 3080 3916 5041 5436 5832 6006
Size: 1846 83 1151 836 1125 395 396 174
Fragments arranged by size:
1846 1151 1125 836 396 395 174 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 812 1876 3582 5034 5238 6006
Size: 812 1064 1706 1452 204 768
Fragments arranged by size:
1706 1452 1064 812 768 204
TatI w'GTAC_w
Cuts at: 0 2424 3878 5142 6006
Size: 2424 1454 1264 864
Fragments arranged by size:
2424 1454 1264 864
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5573 5605 6006
Size: 5573 32 401
Fragments arranged by size:
5573 401 32
Tth111I GACn'n_nGTC
Cuts at: 0 41 4662 5925 6006
Size: 41 4621 1263 81
Fragments arranged by size:
4621 1263 81 41
XbaI T'CTAG_A
Cuts at: 0 5212 6006
Size: 5212 794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 352 2716 5044 5181 5380 6006
Size: 352 2364 2328 137 199 626
Fragments arranged by size:
2364 2328 626 352 199 137
XmnI GAAnn'nnTTC
Cuts at: 0 2351 6006
Size: 2351 3655
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI
NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI
PstI PvuII SacI SexAI SfcI SgrAI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI ApaI AscI
AseI AsiSI AvrII BbeI BcgI BsaHI BsaWI BsiEI
BsiWI BsmI BspEI BsrBI BssHII BstBI BstEII BstZ17I
ClaI DrdI EagI EciI FseI FspAI HaeII HpaI
KasI KpnI MluI NaeI NarI NgoMIV NotI NruI
PacI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SmaI SnaBI SrfI SwaI XhoI XmaI
ZraI