Restriction map for
pF1KB9886
(Linear) (Six-base) MAPSORT of: KIBB9886.seq Check: 6790 from: 1 to: 2175
KIBB9886.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1509 2175
Size: 1509 666
AccI GT'mk_AC
Cuts at: 0 188 2175
Size: 188 1987
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1043 1733 1915 2175
Size: 196 847 690 182 260
Fragments arranged by size:
847 690 260 196 182
AflIII A'CryG_T
Cuts at: 0 746 2175
Size: 746 1429
AleI CACnn'nnGTG
Cuts at: 0 1174 2175
Size: 1174 1001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 538 2175
Size: 538 1637
AlwNI CAG_nnn'CTG
Cuts at: 0 150 174 1312 1441 1451 1555 1593 2175
Size: 150 24 1138 129 10 104 38 582
Fragments arranged by size:
1138 582 150 129 104 38 24 10
ApaI G_GGCC'C
Cuts at: 0 849 2175
Size: 849 1326
ApaLI G'TGCA_C
Cuts at: 0 607 1269 2175
Size: 607 662 906
Fragments arranged by size:
906 662 607
ApoI r'AATT_y
Cuts at: 0 1417 2175
Size: 1417 758
AvaI C'yCGr_G
Cuts at: 0 427 769 893 1402 2175
Size: 427 342 124 509 773
Fragments arranged by size:
773 509 427 342 124
BamHI G'GATC_C
Cuts at: 0 1007 1998 2175
Size: 1007 991 177
Fragments arranged by size:
1007 991 177
BanI G'GyrC_C
Cuts at: 0 433 1348 2175
Size: 433 915 827
Fragments arranged by size:
915 827 433
BanII G_rGCy'C
Cuts at: 0 849 2175
Size: 849 1326
BbsI GAAGACnn'nnnn_
Cuts at: 0 1031 1377 1975 2175
Size: 1031 346 598 200
Fragments arranged by size:
1031 598 346 200
BbvCI CC'TCA_GC
Cuts at: 0 656 2175
Size: 656 1519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 18 52 2175
Size: 18 34 2123
Fragments arranged by size:
2123 34 18
BfrBI ATG'CAT
Cuts at: 0 2032 2128 2175
Size: 2032 96 47
Fragments arranged by size:
2032 96 47
BglI GCCn_nnn'nGGC
Cuts at: 0 410 660 2175
Size: 410 250 1515
Fragments arranged by size:
1515 410 250
BglII A'GATC_T
Cuts at: 0 227 2115 2175
Size: 227 1888 60
Fragments arranged by size:
1888 227 60
BlpI GC'TnA_GC
Cuts at: 0 259 2175
Size: 259 1916
Bme1580I G_kGCm'C
Cuts at: 0 611 849 1273 2175
Size: 611 238 424 902
Fragments arranged by size:
902 611 424 238
BmrI ACTGGGnnnn_n'
Cuts at: 0 1603 2175
Size: 1603 572
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 412 1560 2175
Size: 412 1148 615
Fragments arranged by size:
1148 615 412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 106 259 313 374 547 850 2175
Size: 70 36 153 54 61 173 303 1325
Fragments arranged by size:
1325 303 173 153 70 61 54 36
Bpu10I CC'TnA_GC
Cuts at: 0 656 1574 2175
Size: 656 918 601
Fragments arranged by size:
918 656 601
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 2175
Size: 517 1658
BsaI GGTCTCn'nnnn_
Cuts at: 0 383 2175
Size: 383 1792
BsaAI yAC'GTr
Cuts at: 0 81 2175
Size: 81 2094
BsaHI Gr'CG_yC
Cuts at: 0 532 1744 2175
Size: 532 1212 431
Fragments arranged by size:
1212 532 431
BsaWI w'CCGG_w
Cuts at: 0 451 2175
Size: 451 1724
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2059 2089 2175
Size: 2059 30 86
Fragments arranged by size:
2059 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 376 892 1165 1740 2175
Size: 172 204 516 273 575 435
Fragments arranged by size:
575 516 435 273 204 172
BseYI C'CCAG_C
Cuts at: 0 1260 1826 2175
Size: 1260 566 349
Fragments arranged by size:
1260 566 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1900 2175
Size: 1900 275
BsiHKAI G_wGCw'C
Cuts at: 0 131 611 1273 2175
Size: 131 480 662 902
Fragments arranged by size:
902 662 480 131
BsmI GAATG_Cn'
Cuts at: 0 2034 2130 2175
Size: 2034 96 45
Fragments arranged by size:
2034 96 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 208 523 2175
Size: 154 54 315 1652
Fragments arranged by size:
1652 315 154 54
Bsp1286I G_dGCh'C
Cuts at: 0 131 611 849 1273 2175
Size: 131 480 238 424 902
Fragments arranged by size:
902 480 424 238 131
BspHI T'CATG_A
Cuts at: 0 620 2175
Size: 620 1555
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1082 1509 2106 2175
Size: 1082 427 597 69
Fragments arranged by size:
1082 597 427 69
BsrDI GCAATG_nn'
Cuts at: 0 1040 1581 2175
Size: 1040 541 594
Fragments arranged by size:
1040 594 541
BsrFI r'CCGG_y
Cuts at: 0 131 200 409 2175
Size: 131 69 209 1766
Fragments arranged by size:
1766 209 131 69
BsrGI T'GTAC_A
Cuts at: 0 551 2175
Size: 551 1624
BstAPI GCAn_nnn'nTGC
Cuts at: 0 606 2175
Size: 606 1569
BstBI TT'CG_AA
Cuts at: 0 1187 2175
Size: 1187 988
BstYI r'GATC_y
Cuts at: 0 227 1007 1611 1998 2115 2175
Size: 227 780 604 387 117 60
Fragments arranged by size:
780 604 387 227 117 60
Bsu36I CC'TnA_GG
Cuts at: 0 968 2175
Size: 968 1207
BtgI C'CryG_G
Cuts at: 0 998 1487 1871 2175
Size: 998 489 384 304
Fragments arranged by size:
998 489 384 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 25 1687 2175
Size: 19 6 1662 488
Fragments arranged by size:
1662 488 19 6
ClaI AT'CG_AT
Cuts at: 0 1427 2175
Size: 1427 748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 477 512 2150 2175
Size: 477 35 1638 25
Fragments arranged by size:
1638 477 35 25
DrdI GACnn_nn'nnGTC
Cuts at: 0 1033 2175
Size: 1033 1142
EaeI y'GGCC_r
Cuts at: 0 707 2175
Size: 707 1468
EarI CTCTTCn'nnn_
Cuts at: 0 974 1709 1766 1880 2175
Size: 974 735 57 114 295
Fragments arranged by size:
974 735 295 114 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 2175
Size: 169 2006
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 106 196 259 313 374 547 850
Size: 70 36 90 63 54 61 173 303
Cuts at: 850 1043 1733 1915 2175
Size: 193 690 182 260
Fragments arranged by size:
690 303 260 193 182 173 90 70
63 61 54 36
EcoO109I rG'GnC_Cy
Cuts at: 0 117 561 845 846 1658 1890 2175
Size: 117 444 284 1 812 232 285
Fragments arranged by size:
812 444 285 284 232 117 1
EcoRV GAT'ATC
Cuts at: 0 1602 2175
Size: 1602 573
FspI TGC'GCA
Cuts at: 0 1046 2175
Size: 1046 1129
HaeII r_GCGC'y
Cuts at: 0 151 2175
Size: 151 2024
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1203 1235 1598 1630 2175
Size: 1203 32 363 32 545
Fragments arranged by size:
1203 545 363 32 32
HincII GTy'rAC
Cuts at: 0 1071 2175
Size: 1071 1104
HindIII A'AGCT_T
Cuts at: 0 2012 2175
Size: 2012 163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 2175
Size: 83 2092
MslI CAynn'nnrTG
Cuts at: 0 1174 1492 1498 2175
Size: 1174 318 6 677
Fragments arranged by size:
1174 677 318 6
MspA1I CmG'CkG
Cuts at: 0 48 325 603 660 886 961 1312 1448
Size: 48 277 278 57 226 75 351 136
Cuts at: 1448 1555 2175
Size: 107 620
Fragments arranged by size:
620 351 278 277 226 136 107 75
57 48
NaeI GCC'GGC
Cuts at: 0 411 2175
Size: 411 1764
NcoI C'CATG_G
Cuts at: 0 998 1487 1871 2175
Size: 998 489 384 304
Fragments arranged by size:
998 489 384 304
NgoMIV G'CCGG_C
Cuts at: 0 409 2175
Size: 409 1766
NsiI A_TGCA'T
Cuts at: 0 2034 2130 2175
Size: 2034 96 45
Fragments arranged by size:
2034 96 45
NspI r_CATG'y
Cuts at: 0 750 1497 1972 2175
Size: 750 747 475 203
Fragments arranged by size:
750 747 475 203
PasI CC'CwG_GG
Cuts at: 0 1755 2175
Size: 1755 420
PciI A'CATG_T
Cuts at: 0 746 2175
Size: 746 1429
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 1004 1098 1593 2175
Size: 150 854 94 495 582
Fragments arranged by size:
854 582 495 150 94
PfoI T'CCnGG_A
Cuts at: 0 122 1010 2175
Size: 122 888 1165
Fragments arranged by size:
1165 888 122
PpuMI rG'GwC_Cy
Cuts at: 0 117 561 2175
Size: 117 444 1614
Fragments arranged by size:
1614 444 117
PspOMI G'GGCC_C
Cuts at: 0 845 2175
Size: 845 1330
PspXI vC'TCGA_Gb
Cuts at: 0 893 2175
Size: 893 1282
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1825 1857 2175
Size: 1825 32 318
Fragments arranged by size:
1825 318 32
PstI C_TGCA'G
Cuts at: 0 517 602 2175
Size: 517 85 1573
Fragments arranged by size:
1573 517 85
PvuII CAG'CTG
Cuts at: 0 48 961 1555 2175
Size: 48 913 594 620
Fragments arranged by size:
913 620 594 48
ScaI AGT'ACT
Cuts at: 0 544 2175
Size: 544 1631
SfcI C'TryA_G
Cuts at: 0 513 598 1547 2175
Size: 513 85 949 628
Fragments arranged by size:
949 628 513 85
SmaI CCC'GGG
Cuts at: 0 429 2175
Size: 429 1746
SmlI C'TyrA_G
Cuts at: 0 496 893 1402 2175
Size: 496 397 509 773
Fragments arranged by size:
773 509 496 397
StuI AGG'CCT
Cuts at: 0 1279 2175
Size: 1279 896
StyI C'CwwG_G
Cuts at: 0 917 998 1487 1653 1871 2175
Size: 917 81 489 166 218 304
Fragments arranged by size:
917 489 304 218 166 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 1585 1818 2175
Size: 289 1296 233 357
Fragments arranged by size:
1296 357 289 233
TatI w'GTAC_w
Cuts at: 0 542 551 1808 2158 2175
Size: 542 9 1257 350 17
Fragments arranged by size:
1257 542 350 17 9
XbaI T'CTAG_A
Cuts at: 0 2131 2175
Size: 2131 44
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1869 1878 2175
Size: 1869 9 297
Fragments arranged by size:
1869 297 9
XhoI C'TCGA_G
Cuts at: 0 893 1402 2175
Size: 893 509 773
Fragments arranged by size:
893 773 509
XmaI C'CCGG_G
Cuts at: 0 427 2175
Size: 427 1748
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI ClaI CspCI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PspXI PsrI
PstI PvuII ScaI SfcI SmaI SmlI StuI StyI
TaqII TatI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI
BmgBI BmtI BsaBI BsiEI BsiWI BspEI BsrBI BssHII
BssSI BstEII BstXI BstZ17I BtsI DraI DraIII EagI
EcoICRI EcoNI EcoRI FalI FseI FspAI HpaI KasI
KpnI MfeI MluI MscI NarI NdeI NheI NotI
NruI PacI PmeI PmlI PpiI PshAI PsiI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XmnI ZraI