Restriction map for
pF1KB9883
(Linear) (Six-base) MAPSORT of: KIBB9883.seq Check: 4591 from: 1 to: 1869
KIBB9883.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AclI AA'CG_TT
Cuts at: 0 282 1869
Size: 282 1587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 880 1869
Size: 748 132 989
Fragments arranged by size:
989 748 132
AfeI AGC'GCT
Cuts at: 0 865 1869
Size: 865 1004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 718 1247 1869
Size: 718 529 622
Fragments arranged by size:
718 622 529
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1349 1381 1869
Size: 1349 32 488
Fragments arranged by size:
1349 488 32
AlwNI CAG_nnn'CTG
Cuts at: 0 90 726 1395 1869
Size: 90 636 669 474
Fragments arranged by size:
669 636 474 90
ApaLI G'TGCA_C
Cuts at: 0 1561 1869
Size: 1561 308
ApoI r'AATT_y
Cuts at: 0 1511 1869
Size: 1511 358
AvaI C'yCGr_G
Cuts at: 0 131 1020 1869
Size: 131 889 849
Fragments arranged by size:
889 849 131
BanI G'GyrC_C
Cuts at: 0 311 1130 1223 1869
Size: 311 819 93 646
Fragments arranged by size:
819 646 311 93
BanII G_rGCy'C
Cuts at: 0 712 973 1066 1276 1337 1604 1869
Size: 712 261 93 210 61 267 265
Fragments arranged by size:
712 267 265 261 210 93 61
BbeI G_GCGC'C
Cuts at: 0 315 1134 1869
Size: 315 819 735
Fragments arranged by size:
819 735 315
BbsI GAAGACnn'nnnn_
Cuts at: 0 569 1869
Size: 569 1300
BbvCI CC'TCA_GC
Cuts at: 0 1328 1420 1869
Size: 1328 92 449
Fragments arranged by size:
1328 449 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1100 1134 1869
Size: 1100 34 735
Fragments arranged by size:
1100 735 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 809 1869
Size: 809 1060
BclI T'GATC_A
Cuts at: 0 1815 1869
Size: 1815 54
BglI GCCn_nnn'nGGC
Cuts at: 0 213 1869
Size: 213 1656
Bme1580I G_kGCm'C
Cuts at: 0 587 1019 1565 1869
Size: 587 432 546 304
Fragments arranged by size:
587 546 432 304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1814 1869
Size: 1814 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 913 949 1102 1761 1869
Size: 675 238 36 153 659 108
Fragments arranged by size:
675 659 238 153 108 36
Bpu10I CC'TnA_GC
Cuts at: 0 253 842 974 1270 1328 1420 1869
Size: 253 589 132 296 58 92 449
Fragments arranged by size:
589 449 296 253 132 92 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1869
Size: 784 1085
BsaI GGTCTCn'nnnn_
Cuts at: 0 500 1869
Size: 500 1369
BsaAI yAC'GTr
Cuts at: 0 1560 1869
Size: 1560 309
BsaHI Gr'CG_yC
Cuts at: 0 312 1131 1869
Size: 312 819 738
Fragments arranged by size:
819 738 312
BsaWI w'CCGG_w
Cuts at: 0 106 1869
Size: 106 1763
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1148 1178 1258 1288 1648 1678 1869
Size: 1148 30 80 30 360 30 191
Fragments arranged by size:
1148 360 191 80 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 467 868 1209 1345 1801 1869
Size: 467 401 341 136 456 68
Fragments arranged by size:
467 456 401 341 136 68
BseYI C'CCAG_C
Cuts at: 0 23 90 163 374 386 677 1835 1869
Size: 23 67 73 211 12 291 1158 34
Fragments arranged by size:
1158 291 211 73 67 34 23 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1606 1735 1869
Size: 163 1443 129 134
Fragments arranged by size:
1443 163 134 129
BsiEI CG_ry'CG
Cuts at: 0 394 1869
Size: 394 1475
BsiHKAI G_wGCw'C
Cuts at: 0 130 226 1109 1276 1337 1565 1604 1869
Size: 130 96 883 167 61 228 39 265
Fragments arranged by size:
883 265 228 167 130 96 61 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 802 1869
Size: 802 1067
Bsp1286I G_dGCh'C
Cuts at: 0 130 226 587 712 973 1019 1066 1109
Size: 130 96 361 125 261 46 47 43
Cuts at: 1109 1276 1337 1565 1604 1869
Size: 167 61 228 39 265
Fragments arranged by size:
361 265 261 228 167 130 125 96
61 47 46 43 39
BspEI T'CCGG_A
Cuts at: 0 106 1869
Size: 106 1763
BsrBI CCG'CTC
Cuts at: 0 573 897 1407 1869
Size: 573 324 510 462
Fragments arranged by size:
573 510 462 324
BsrFI r'CCGG_y
Cuts at: 0 296 905 1031 1869
Size: 296 609 126 838
Fragments arranged by size:
838 609 296 126
BssHII G'CGCG_C
Cuts at: 0 1090 1092 1470 1869
Size: 1090 2 378 399
Fragments arranged by size:
1090 399 378 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1182 1713 1869
Size: 1182 531 156
Fragments arranged by size:
1182 531 156
BstYI r'GATC_y
Cuts at: 0 39 1538 1869
Size: 39 1499 331
Fragments arranged by size:
1499 331 39
Bsu36I CC'TnA_GG
Cuts at: 0 1339 1396 1612 1769 1869
Size: 1339 57 216 157 100
Fragments arranged by size:
1339 216 157 100 57
BtgI C'CryG_G
Cuts at: 0 55 202 1235 1869
Size: 55 147 1033 634
Fragments arranged by size:
1033 634 147 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 272 1869
Size: 272 1597
BtsI GCAGTG_nn'
Cuts at: 0 232 524 940 1869
Size: 232 292 416 929
Fragments arranged by size:
929 416 292 232
DraIII CAC_nnn'GTG
Cuts at: 0 125 502 1869
Size: 125 377 1367
Fragments arranged by size:
1367 377 125
DrdI GACnn_nn'nnGTC
Cuts at: 0 539 1869
Size: 539 1330
EaeI y'GGCC_r
Cuts at: 0 391 1869
Size: 391 1478
EagI C'GGCC_G
Cuts at: 0 391 1869
Size: 391 1478
EarI CTCTTCn'nnn_
Cuts at: 0 625 724 737 1439 1869
Size: 625 99 13 702 430
Fragments arranged by size:
702 625 430 99 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 1543 1869
Size: 80 1463 326
Fragments arranged by size:
1463 326 80
EcoICRI GAG'CTC
Cuts at: 0 1274 1335 1602 1869
Size: 1274 61 267 267
Fragments arranged by size:
1274 267 267 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 748 880 913 949 1102 1761 1869
Size: 675 73 132 33 36 153 659 108
Fragments arranged by size:
675 659 153 132 108 73 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1399 1869
Size: 1399 470
EcoO109I rG'GnC_Cy
Cuts at: 0 272 723 1302 1392 1869
Size: 272 451 579 90 477
Fragments arranged by size:
579 477 451 272 90
EcoRI G'AATT_C
Cuts at: 0 1511 1869
Size: 1511 358
FspI TGC'GCA
Cuts at: 0 1504 1869
Size: 1504 365
HaeII r_GCGC'y
Cuts at: 0 315 867 906 1134 1798 1869
Size: 315 552 39 228 664 71
Fragments arranged by size:
664 552 315 228 71 39
KasI G'GCGC_C
Cuts at: 0 311 1130 1869
Size: 311 819 739
Fragments arranged by size:
819 739 311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1566 1869
Size: 1566 303
MslI CAynn'nnrTG
Cuts at: 0 1229 1869
Size: 1229 640
MspA1I CmG'CkG
Cuts at: 0 57 87 204 378 390 1188 1347 1371
Size: 57 30 117 174 12 798 159 24
Cuts at: 1371 1869
Size: 498
Fragments arranged by size:
798 498 174 159 117 57 30 24
12
NaeI GCC'GGC
Cuts at: 0 907 1869
Size: 907 962
NarI GG'CG_CC
Cuts at: 0 312 1131 1869
Size: 312 819 738
Fragments arranged by size:
819 738 312
NgoMIV G'CCGG_C
Cuts at: 0 905 1869
Size: 905 964
NotI GC'GGCC_GC
Cuts at: 0 391 1869
Size: 391 1478
NspI r_CATG'y
Cuts at: 0 1234 1869
Size: 1234 635
PasI CC'CwG_GG
Cuts at: 0 423 1869
Size: 423 1446
PflMI CCAn_nnn'nTGG
Cuts at: 0 684 1869
Size: 684 1185
PfoI T'CCnGG_A
Cuts at: 0 286 1166 1256 1595 1748 1777 1869
Size: 286 880 90 339 153 29 92
Fragments arranged by size:
880 339 286 153 92 90 29
PmlI CAC'GTG
Cuts at: 0 1560 1869
Size: 1560 309
PpuMI rG'GwC_Cy
Cuts at: 0 723 1302 1869
Size: 723 579 567
Fragments arranged by size:
723 579 567
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1849 1869
Size: 1849 20
PstI C_TGCA'G
Cuts at: 0 830 1187 1193 1869
Size: 830 357 6 676
Fragments arranged by size:
830 676 357 6
PvuII CAG'CTG
Cuts at: 0 378 1188 1347 1869
Size: 378 810 159 522
Fragments arranged by size:
810 522 378 159
SacI G_AGCT'C
Cuts at: 0 1276 1337 1604 1869
Size: 1276 61 267 265
Fragments arranged by size:
1276 267 265 61
SacII CC_GC'GG
Cuts at: 0 58 205 1869
Size: 58 147 1664
Fragments arranged by size:
1664 147 58
SapI GCTCTTCn'nnn_
Cuts at: 0 724 1439 1869
Size: 724 715 430
Fragments arranged by size:
724 715 430
SexAI A'CCwGG_T
Cuts at: 0 647 1869
Size: 647 1222
SfcI C'TryA_G
Cuts at: 0 826 1183 1189 1869
Size: 826 357 6 680
Fragments arranged by size:
826 680 357 6
SfoI GGC'GCC
Cuts at: 0 313 1132 1869
Size: 313 819 737
Fragments arranged by size:
819 737 313
SmaI CCC'GGG
Cuts at: 0 133 1022 1869
Size: 133 889 847
Fragments arranged by size:
889 847 133
SmlI C'TyrA_G
Cuts at: 0 799 1869
Size: 799 1070
StuI AGG'CCT
Cuts at: 0 847 1774 1869
Size: 847 927 95
Fragments arranged by size:
927 847 95
StyI C'CwwG_G
Cuts at: 0 683 1322 1728 1869
Size: 683 639 406 141
Fragments arranged by size:
683 639 406 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 733 1869
Size: 733 1136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 132 164 1018 1050 1648 1680 1869
Size: 132 32 854 32 598 32 189
Fragments arranged by size:
854 598 189 132 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 87 1869
Size: 87 1782
XmaI C'CCGG_G
Cuts at: 0 131 1020 1869
Size: 131 889 849
Fragments arranged by size:
889 849 131
Enzymes that do cut:
AclI AcuI AfeI AlfI AloI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII BstAPI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PasI PflMI PfoI PmlI PpuMI
PsrI PstI PvuII SacI SacII SapI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BfrBI BglII BlpI BmgBI BmrI BmtI BsaBI BsiWI
BsmI BspHI BspMI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI