Restriction map for
pF1KB9877
(Linear) (Six-base) MAPSORT of: KIBB9877.seq Check: 8330 from: 1 to: 1638
KIBB9877.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 33 1638
Size: 33 1605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1638
Size: 292 1346
AfeI AGC'GCT
Cuts at: 0 279 292 1638
Size: 279 13 1346
Fragments arranged by size:
1346 279 13
AlwNI CAG_nnn'CTG
Cuts at: 0 1278 1638
Size: 1278 360
ApaI G_GGCC'C
Cuts at: 0 550 1638
Size: 550 1088
AvaI C'yCGr_G
Cuts at: 0 429 541 565 969 1157 1265 1486 1638
Size: 429 112 24 404 188 108 221 152
Fragments arranged by size:
429 404 221 188 152 112 108 24
AvrII C'CTAG_G
Cuts at: 0 1033 1638
Size: 1033 605
BanI G'GyrC_C
Cuts at: 0 783 999 1029 1542 1638
Size: 783 216 30 513 96
Fragments arranged by size:
783 513 216 96 30
BanII G_rGCy'C
Cuts at: 0 364 452 550 557 809 976 1638
Size: 364 88 98 7 252 167 662
Fragments arranged by size:
662 364 252 167 98 88 7
BbeI G_GCGC'C
Cuts at: 0 1546 1638
Size: 1546 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 789 1638
Size: 789 849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 765 799 1638
Size: 765 34 839
Fragments arranged by size:
839 765 34
Bme1580I G_kGCm'C
Cuts at: 0 550 566 680 788 1638
Size: 550 16 114 108 850
Fragments arranged by size:
850 550 114 108 16
BmgBI CAC'GTC
Cuts at: 0 1062 1638
Size: 1062 576
BmrI ACTGGGnnnn_n'
Cuts at: 0 434 1638
Size: 434 1204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 383 1638
Size: 383 1255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 960 979 1177 1638
Size: 898 62 19 198 461
Fragments arranged by size:
898 461 198 62 19
Bpu10I CC'TnA_GC
Cuts at: 0 377 551 602 1638
Size: 377 174 51 1036
Fragments arranged by size:
1036 377 174 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 440 1638
Size: 414 26 1198
Fragments arranged by size:
1198 414 26
BsaAI yAC'GTr
Cuts at: 0 183 1312 1638
Size: 183 1129 326
Fragments arranged by size:
1129 326 183
BsaHI Gr'CG_yC
Cuts at: 0 1049 1111 1262 1372 1543 1638
Size: 1049 62 151 110 171 95
Fragments arranged by size:
1049 171 151 110 95 62
BsaWI w'CCGG_w
Cuts at: 0 1044 1638
Size: 1044 594
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 958 988 1213 1243 1638
Size: 958 30 225 30 395
Fragments arranged by size:
958 395 225 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 1638
Size: 286 1352
BseYI C'CCAG_C
Cuts at: 0 483 618 787 1324 1638
Size: 483 135 169 537 314
Fragments arranged by size:
537 483 314 169 135
BsiEI CG_ry'CG
Cuts at: 0 755 1332 1638
Size: 755 577 306
Fragments arranged by size:
755 577 306
BsiHKAI G_wGCw'C
Cuts at: 0 233 383 809 1226 1638
Size: 233 150 426 417 412
Fragments arranged by size:
426 417 412 233 150
Bsp1286I G_dGCh'C
Cuts at: 0 233 364 383 452 550 557 566 680
Size: 233 131 19 69 98 7 9 114
Cuts at: 680 788 809 976 1226 1638
Size: 108 21 167 250 412
Fragments arranged by size:
412 250 233 167 131 114 108 98
69 21 19 9 7
BspEI T'CCGG_A
Cuts at: 0 1044 1638
Size: 1044 594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 1256 1638
Size: 33 1223 382
Fragments arranged by size:
1223 382 33
BsrBI CCG'CTC
Cuts at: 0 315 526 601 1514 1638
Size: 315 211 75 913 124
Fragments arranged by size:
913 315 211 124 75
BsrDI GCAATG_nn'
Cuts at: 0 1185 1638
Size: 1185 453
BsrFI r'CCGG_y
Cuts at: 0 754 960 1532 1638
Size: 754 206 572 106
Fragments arranged by size:
754 572 206 106
BssHII G'CGCG_C
Cuts at: 0 24 1638
Size: 24 1614
BssSI C'ACGA_G
Cuts at: 0 232 1225 1638
Size: 232 993 413
Fragments arranged by size:
993 413 232
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1278 1638
Size: 1278 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1581 1638
Size: 1581 57
Bsu36I CC'TnA_GG
Cuts at: 0 269 1638
Size: 269 1369
BtgI C'CryG_G
Cuts at: 0 690 1547 1638
Size: 690 857 91
Fragments arranged by size:
857 690 91
BtsI GCAGTG_nn'
Cuts at: 0 35 1024 1638
Size: 35 989 614
Fragments arranged by size:
989 614 35
EaeI y'GGCC_r
Cuts at: 0 53 752 1329 1364 1595 1638
Size: 53 699 577 35 231 43
Fragments arranged by size:
699 577 231 53 43 35
EagI C'GGCC_G
Cuts at: 0 752 1329 1638
Size: 752 577 309
Fragments arranged by size:
752 577 309
EarI CTCTTCn'nnn_
Cuts at: 0 311 576 1638
Size: 311 265 1062
Fragments arranged by size:
1062 311 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 93 148 756 1638
Size: 93 55 608 882
Fragments arranged by size:
882 608 93 55
EcoICRI GAG'CTC
Cuts at: 0 807 1638
Size: 807 831
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 898 960 979 1177 1638
Size: 292 606 62 19 198 461
Fragments arranged by size:
606 461 292 198 62 19
EcoO109I rG'GnC_Cy
Cuts at: 0 264 507 546 547 813 1071 1638
Size: 264 243 39 1 266 258 567
Fragments arranged by size:
567 266 264 258 243 39 1
HaeII r_GCGC'y
Cuts at: 0 165 281 294 471 761 1546 1638
Size: 165 116 13 177 290 785 92
Fragments arranged by size:
785 290 177 165 116 92 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 1101 1133 1213 1245 1638
Size: 838 32 231 32 80 32 393
Fragments arranged by size:
838 393 231 80 32 32 32
KasI G'GCGC_C
Cuts at: 0 1542 1638
Size: 1542 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1638
Size: 538 1100
MscI TGG'CCA
Cuts at: 0 55 1366 1597 1638
Size: 55 1311 231 41
Fragments arranged by size:
1311 231 55 41
MspA1I CmG'CkG
Cuts at: 0 487 559 692 1275 1328 1417 1549 1590
Size: 487 72 133 583 53 89 132 41
Cuts at: 1590 1638
Size: 48
Fragments arranged by size:
583 487 133 132 89 72 53 48
41
NaeI GCC'GGC
Cuts at: 0 756 1638
Size: 756 882
NarI GG'CG_CC
Cuts at: 0 1543 1638
Size: 1543 95
NgoMIV G'CCGG_C
Cuts at: 0 754 1638
Size: 754 884
NruI TCG'CGA
Cuts at: 0 446 1638
Size: 446 1192
PasI CC'CwG_GG
Cuts at: 0 645 1638
Size: 645 993
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 1638
Size: 183 1455
PfoI T'CCnGG_A
Cuts at: 0 722 914 1193 1387 1638
Size: 722 192 279 194 251
Fragments arranged by size:
722 279 251 194 192
PmlI CAC'GTG
Cuts at: 0 183 1312 1638
Size: 183 1129 326
Fragments arranged by size:
1129 326 183
PpuMI rG'GwC_Cy
Cuts at: 0 264 507 813 1071 1638
Size: 264 243 306 258 567
Fragments arranged by size:
567 306 264 258 243
PspOMI G'GGCC_C
Cuts at: 0 546 1638
Size: 546 1092
PstI C_TGCA'G
Cuts at: 0 44 749 1410 1638
Size: 44 705 661 228
Fragments arranged by size:
705 661 228 44
PvuII CAG'CTG
Cuts at: 0 487 1638
Size: 487 1151
SacI G_AGCT'C
Cuts at: 0 809 1638
Size: 809 829
SacII CC_GC'GG
Cuts at: 0 693 1550 1638
Size: 693 857 88
Fragments arranged by size:
857 693 88
SanDI GG'GwC_CC
Cuts at: 0 507 813 1071 1638
Size: 507 306 258 567
Fragments arranged by size:
567 507 306 258
SapI GCTCTTCn'nnn_
Cuts at: 0 311 1638
Size: 311 1327
SfcI C'TryA_G
Cuts at: 0 40 745 1406 1638
Size: 40 705 661 232
Fragments arranged by size:
705 661 232 40
SfoI GGC'GCC
Cuts at: 0 1544 1638
Size: 1544 94
SmaI CCC'GGG
Cuts at: 0 567 1638
Size: 567 1071
SmlI C'TyrA_G
Cuts at: 0 1157 1638
Size: 1157 481
SspI AAT'ATT
Cuts at: 0 88 1638
Size: 88 1550
StuI AGG'CCT
Cuts at: 0 325 826 981 1638
Size: 325 501 155 657
Fragments arranged by size:
657 501 325 155
StyI C'CwwG_G
Cuts at: 0 1033 1598 1638
Size: 1033 565 40
Fragments arranged by size:
1033 565 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 254 1638
Size: 254 1384
TatI w'GTAC_w
Cuts at: 0 965 1638
Size: 965 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 929 961 1638
Size: 670 32 227 32 677
Fragments arranged by size:
677 670 227 32 32
XhoI C'TCGA_G
Cuts at: 0 1157 1638
Size: 1157 481
XmaI C'CCGG_G
Cuts at: 0 565 1638
Size: 565 1073
XmnI GAAnn'nnTTC
Cuts at: 0 1094 1638
Size: 1094 544
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI AvrII BanI
BanII BbeI BbsI BcgI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI Bsu36I BtgI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MscI MspA1I NaeI NarI NgoMIV
NruI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SapI SfcI SfoI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmtI BpuEI BsaBI BsgI BsiWI BsmI BsmBI
BspHI BsrGI BstBI BstEII BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MslI NcoI NdeI NheI NotI NsiI NspI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XcmI ZraI