Restriction map for
pF1KB9873
(Linear) (Six-base) MAPSORT of: KIBB9873.seq Check: 9911 from: 1 to: 1497
KIBB9873.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1180 1497
Size: 1180 317
Acc65I G'GTAC_C
Cuts at: 0 632 1497
Size: 632 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1497
Size: 350 1147
AgeI A'CCGG_T
Cuts at: 0 1212 1497
Size: 1212 285
AhdI GACnn_n'nnGTC
Cuts at: 0 1301 1497
Size: 1301 196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1497
Size: 1124 32 341
Fragments arranged by size:
1124 341 32
AlwNI CAG_nnn'CTG
Cuts at: 0 481 539 1497
Size: 481 58 958
Fragments arranged by size:
958 481 58
ApoI r'AATT_y
Cuts at: 0 335 706 1497
Size: 335 371 791
Fragments arranged by size:
791 371 335
BanI G'GyrC_C
Cuts at: 0 50 632 1497
Size: 50 582 865
Fragments arranged by size:
865 582 50
BanII G_rGCy'C
Cuts at: 0 475 1497
Size: 475 1022
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 1497
Size: 584 913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 19 53 1497
Size: 19 34 1444
Fragments arranged by size:
1444 34 19
BclI T'GATC_A
Cuts at: 0 107 1497
Size: 107 1390
BfrBI ATG'CAT
Cuts at: 0 585 1497
Size: 585 912
BlpI GC'TnA_GC
Cuts at: 0 842 1497
Size: 842 655
Bme1580I G_kGCm'C
Cuts at: 0 53 314 1497
Size: 53 261 1183
Fragments arranged by size:
1183 261 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 150 1062 1497
Size: 150 912 435
Fragments arranged by size:
912 435 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1497
Size: 925 572
Bpu10I CC'TnA_GC
Cuts at: 0 150 474 1497
Size: 150 324 1023
Fragments arranged by size:
1023 324 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 11 127 160 943 1497
Size: 11 116 33 783 554
Fragments arranged by size:
783 554 116 33 11
BsaBI GATnn'nnATC
Cuts at: 0 112 520 1497
Size: 112 408 977
Fragments arranged by size:
977 408 112
BsaWI w'CCGG_w
Cuts at: 0 13 887 1212 1497
Size: 13 874 325 285
Fragments arranged by size:
874 325 285 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1124 1154 1497
Size: 1124 30 343
Fragments arranged by size:
1124 343 30
BseYI C'CCAG_C
Cuts at: 0 21 137 351 733 1075 1497
Size: 21 116 214 382 342 422
Fragments arranged by size:
422 382 342 214 116 21
BsiEI CG_ry'CG
Cuts at: 0 237 1497
Size: 237 1260
BsiHKAI G_wGCw'C
Cuts at: 0 475 1497
Size: 475 1022
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1113 1497
Size: 1113 384
Bsp1286I G_dGCh'C
Cuts at: 0 53 314 475 1497
Size: 53 261 161 1022
Fragments arranged by size:
1022 261 161 53
BspHI T'CATG_A
Cuts at: 0 761 1497
Size: 761 736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1180 1497
Size: 1180 317
BsrDI GCAATG_nn'
Cuts at: 0 550 1497
Size: 550 947
BsrFI r'CCGG_y
Cuts at: 0 742 1212 1497
Size: 742 470 285
Fragments arranged by size:
742 470 285
BssSI C'ACGA_G
Cuts at: 0 85 1497
Size: 85 1412
BstEII G'GTnAC_C
Cuts at: 0 597 1497
Size: 597 900
BstXI CCAn_nnnn'nTGG
Cuts at: 0 867 1497
Size: 867 630
BstYI r'GATC_y
Cuts at: 0 1310 1379 1497
Size: 1310 69 118
Fragments arranged by size:
1310 118 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 379 1497
Size: 379 1118
EaeI y'GGCC_r
Cuts at: 0 8 782 869 1497
Size: 8 774 87 628
Fragments arranged by size:
774 628 87 8
EarI CTCTTCn'nnn_
Cuts at: 0 401 1009 1497
Size: 401 608 488
Fragments arranged by size:
608 488 401
EcoICRI GAG'CTC
Cuts at: 0 473 1497
Size: 473 1024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 925 1497
Size: 350 575 572
Fragments arranged by size:
575 572 350
EcoO109I rG'GnC_Cy
Cuts at: 0 180 478 1497
Size: 180 298 1019
Fragments arranged by size:
1019 298 180
EcoRI G'AATT_C
Cuts at: 0 706 1497
Size: 706 791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 466 1497
Size: 466 1031
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 936 968 1048 1080 1287 1319
Size: 310 32 594 32 80 32 207 32
Cuts at: 1319 1497
Size: 178
Fragments arranged by size:
594 310 207 178 80 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1199 1487 1497
Size: 1199 288 10
Fragments arranged by size:
1199 288 10
HpaI GTT'AAC
Cuts at: 0 1199 1487 1497
Size: 1199 288 10
Fragments arranged by size:
1199 288 10
KpnI G_GTAC'C
Cuts at: 0 636 1497
Size: 636 861
MfeI C'AATT_G
Cuts at: 0 527 1497
Size: 527 970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1168 1497
Size: 641 527 329
Fragments arranged by size:
641 527 329
MscI TGG'CCA
Cuts at: 0 10 784 871 1497
Size: 10 774 87 626
Fragments arranged by size:
774 626 87 10
MslI CAynn'nnrTG
Cuts at: 0 590 1497
Size: 590 907
NaeI GCC'GGC
Cuts at: 0 744 1497
Size: 744 753
NgoMIV G'CCGG_C
Cuts at: 0 742 1497
Size: 742 755
NsiI A_TGCA'T
Cuts at: 0 587 1497
Size: 587 910
NspI r_CATG'y
Cuts at: 0 585 1497
Size: 585 912
PasI CC'CwG_GG
Cuts at: 0 46 807 1497
Size: 46 761 690
Fragments arranged by size:
761 690 46
PfoI T'CCnGG_A
Cuts at: 0 563 1382 1497
Size: 563 819 115
Fragments arranged by size:
819 563 115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1124 1156 1497
Size: 1054 32 38 32 341
Fragments arranged by size:
1054 341 38 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 180 1497
Size: 180 1317
PvuI CG_AT'CG
Cuts at: 0 237 1497
Size: 237 1260
SacI G_AGCT'C
Cuts at: 0 475 1497
Size: 475 1022
SanDI GG'GwC_CC
Cuts at: 0 180 1497
Size: 180 1317
SapI GCTCTTCn'nnn_
Cuts at: 0 401 1497
Size: 401 1096
ScaI AGT'ACT
Cuts at: 0 226 1497
Size: 226 1271
SfcI C'TryA_G
Cuts at: 0 1121 1497
Size: 1121 376
SspI AAT'ATT
Cuts at: 0 1473 1497
Size: 1473 24
StyI C'CwwG_G
Cuts at: 0 1448 1497
Size: 1448 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 68 1497
Size: 68 1429
TatI w'GTAC_w
Cuts at: 0 224 266 1497
Size: 224 42 1231
Fragments arranged by size:
1231 224 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 598 630 857 889 1151 1183 1497
Size: 598 32 227 32 262 32 314
Fragments arranged by size:
598 314 262 227 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1091 1446 1497
Size: 1091 355 51
Fragments arranged by size:
1091 355 51
XmnI GAAnn'nnTTC
Cuts at: 0 335 1497
Size: 335 1162
Enzymes that do cut:
AarI Acc65I AcuI AgeI AhdI AloI AlwNI ApoI
BanI BanII BbsI BcgI BclI BfrBI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BssSI BstEII BstXI BstYI BtgZI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII HpaI KpnI
MfeI MmeI MscI MslI NaeI NgoMIV NsiI NspI
PasI PfoI PpiI PpuMI PvuI SacI SanDI SapI
ScaI SfcI SspI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BciVI BglI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaHI BseRI BsgI BsiWI BsmI
BspEI BsrBI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRV FseI FspI FspAI HaeII HindIII
KasI MluI MspA1I NarI NcoI NdeI NheI NotI
NruI PacI PciI PflMI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PstI PvuII RsrII SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XhoI XmaI ZraI