Restriction map for
pF1KB9871
(Linear) (Six-base) MAPSORT of: KIBB9871.seq Check: 4321 from: 1 to: 1470
KIBB9871.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 818 1000 1136 1226 1470
Size: 818 182 136 90 244
Fragments arranged by size:
818 244 182 136 90
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 1470
Size: 771 699
AfeI AGC'GCT
Cuts at: 0 924 1186 1243 1270 1470
Size: 924 262 57 27 200
Fragments arranged by size:
924 262 200 57 27
AleI CACnn'nnGTG
Cuts at: 0 502 1470
Size: 502 968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1078 1470
Size: 1078 392
ApaI G_GGCC'C
Cuts at: 0 1033 1470
Size: 1033 437
ApaLI G'TGCA_C
Cuts at: 0 493 1470
Size: 493 977
AvaI C'yCGr_G
Cuts at: 0 579 1080 1347 1441 1447 1470
Size: 579 501 267 94 6 23
Fragments arranged by size:
579 501 267 94 23 6
BanI G'GyrC_C
Cuts at: 0 616 796 936 1214 1451 1470
Size: 616 180 140 278 237 19
Fragments arranged by size:
616 278 237 180 140 19
BanII G_rGCy'C
Cuts at: 0 610 719 913 952 1033 1348 1448 1470
Size: 610 109 194 39 81 315 100 22
Fragments arranged by size:
610 315 194 109 100 81 39 22
BbeI G_GCGC'C
Cuts at: 0 620 800 940 1470
Size: 620 180 140 530
Fragments arranged by size:
620 530 180 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1312 1470
Size: 1312 158
BbvCI CC'TCA_GC
Cuts at: 0 963 1291 1470
Size: 963 328 179
Fragments arranged by size:
963 328 179
BlpI GC'TnA_GC
Cuts at: 0 814 1470
Size: 814 656
Bme1580I G_kGCm'C
Cuts at: 0 497 1033 1454 1470
Size: 497 536 421 16
Fragments arranged by size:
536 497 421 16
BmgBI CAC'GTC
Cuts at: 0 636 657 1470
Size: 636 21 813
Fragments arranged by size:
813 636 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 1470
Size: 624 846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 267 288 333 606 1470
Size: 162 105 21 45 273 864
Fragments arranged by size:
864 273 162 105 45 21
Bpu10I CC'TnA_GC
Cuts at: 0 963 1291 1470
Size: 963 328 179
Fragments arranged by size:
963 328 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 1102 1470
Size: 138 964 368
Fragments arranged by size:
964 368 138
BsaAI yAC'GTr
Cuts at: 0 1302 1470
Size: 1302 168
BsaHI Gr'CG_yC
Cuts at: 0 617 797 937 1470
Size: 617 180 140 533
Fragments arranged by size:
617 533 180 140
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 96 432 792 951 1470
Size: 39 57 336 360 159 519
Fragments arranged by size:
519 360 336 159 57 39
BseYI C'CCAG_C
Cuts at: 0 34 925 942 1026 1470
Size: 34 891 17 84 444
Fragments arranged by size:
891 444 84 34 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 682 715 1048 1470
Size: 679 3 33 333 422
Fragments arranged by size:
679 422 333 33 3
BsiEI CG_ry'CG
Cuts at: 0 215 614 984 1470
Size: 215 399 370 486
Fragments arranged by size:
486 399 370 215
BsiHKAI G_wGCw'C
Cuts at: 0 497 1391 1470
Size: 497 894 79
Fragments arranged by size:
894 497 79
BsiWI C'GTAC_G
Cuts at: 0 551 1470
Size: 551 919
BsmBI CGTCTCn'nnnn_
Cuts at: 0 47 1470
Size: 47 1423
Bsp1286I G_dGCh'C
Cuts at: 0 497 610 719 913 952 1033 1348 1391
Size: 497 113 109 194 39 81 315 43
Cuts at: 1391 1448 1454 1470
Size: 57 6 16
Fragments arranged by size:
497 315 194 113 109 81 57 43
39 16 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 818 1000 1136 1226 1470
Size: 818 182 136 90 244
Fragments arranged by size:
818 244 182 136 90
BsrBI CCG'CTC
Cuts at: 0 741 1023 1149 1470
Size: 741 282 126 321
Fragments arranged by size:
741 321 282 126
BsrFI r'CCGG_y
Cuts at: 0 474 613 643 898 1231 1470
Size: 474 139 30 255 333 239
Fragments arranged by size:
474 333 255 239 139 30
BtgI C'CryG_G
Cuts at: 0 215 227 236 529 1397 1470
Size: 215 12 9 293 868 73
Fragments arranged by size:
868 293 215 73 12 9
EaeI y'GGCC_r
Cuts at: 0 212 611 896 981 1470
Size: 212 399 285 85 489
Fragments arranged by size:
489 399 285 212 85
EagI C'GGCC_G
Cuts at: 0 212 611 981 1470
Size: 212 399 370 489
Fragments arranged by size:
489 399 370 212
EarI CTCTTCn'nnn_
Cuts at: 0 72 120 1470
Size: 72 48 1350
Fragments arranged by size:
1350 72 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 111 1470
Size: 111 1359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 267 288 333 606 771 1470
Size: 162 105 21 45 273 165 699
Fragments arranged by size:
699 273 165 162 105 45 21
EcoO109I rG'GnC_Cy
Cuts at: 0 173 387 1030 1059 1470
Size: 173 214 643 29 411
Fragments arranged by size:
643 411 214 173 29
FspI TGC'GCA
Cuts at: 0 1279 1470
Size: 1279 191
HaeII r_GCGC'y
Cuts at: 0 290 314 620 800 926 940 1188 1245
Size: 290 24 306 180 126 14 248 57
Cuts at: 1245 1272 1397 1470
Size: 27 125 73
Fragments arranged by size:
306 290 248 180 126 125 73 57
27 24 14
HindIII A'AGCT_T
Cuts at: 0 156 1470
Size: 156 1314
KasI G'GCGC_C
Cuts at: 0 616 796 936 1470
Size: 616 180 140 534
Fragments arranged by size:
616 534 180 140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1223 1470
Size: 275 948 247
Fragments arranged by size:
948 275 247
MslI CAynn'nnrTG
Cuts at: 0 502 1470
Size: 502 968
MspA1I CmG'CkG
Cuts at: 0 128 217 229 238 263 410 531 626
Size: 128 89 12 9 25 147 121 95
Cuts at: 626 794 894 942 1070 1212 1355 1399 1470
Size: 168 100 48 128 142 143 44 71
Fragments arranged by size:
168 147 143 142 128 128 121 100
95 89 71 48 44 25 12 9
NaeI GCC'GGC
Cuts at: 0 615 645 900 1470
Size: 615 30 255 570
Fragments arranged by size:
615 570 255 30
NarI GG'CG_CC
Cuts at: 0 617 797 937 1470
Size: 617 180 140 533
Fragments arranged by size:
617 533 180 140
NgoMIV G'CCGG_C
Cuts at: 0 613 643 898 1470
Size: 613 30 255 572
Fragments arranged by size:
613 572 255 30
NotI GC'GGCC_GC
Cuts at: 0 212 1470
Size: 212 1258
PflMI CCAn_nnn'nTGG
Cuts at: 0 629 1470
Size: 629 841
PfoI T'CCnGG_A
Cuts at: 0 178 1470
Size: 178 1292
PmlI CAC'GTG
Cuts at: 0 1302 1470
Size: 1302 168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 1470
Size: 175 32 1263
Fragments arranged by size:
1263 175 32
PspOMI G'GGCC_C
Cuts at: 0 1029 1470
Size: 1029 441
PstI C_TGCA'G
Cuts at: 0 518 1211 1436 1470
Size: 518 693 225 34
Fragments arranged by size:
693 518 225 34
PvuII CAG'CTG
Cuts at: 0 128 263 626 1070 1470
Size: 128 135 363 444 400
Fragments arranged by size:
444 400 363 135 128
SacII CC_GC'GG
Cuts at: 0 218 230 239 532 1400 1470
Size: 218 12 9 293 868 70
Fragments arranged by size:
868 293 218 70 12 9
SapI GCTCTTCn'nnn_
Cuts at: 0 72 1470
Size: 72 1398
SfcI C'TryA_G
Cuts at: 0 514 1207 1432 1470
Size: 514 693 225 38
Fragments arranged by size:
693 514 225 38
SfoI GGC'GCC
Cuts at: 0 618 798 938 1470
Size: 618 180 140 532
Fragments arranged by size:
618 532 180 140
SgrAI Cr'CCGG_yG
Cuts at: 0 1231 1470
Size: 1231 239
SmaI CCC'GGG
Cuts at: 0 581 1082 1449 1470
Size: 581 501 367 21
Fragments arranged by size:
581 501 367 21
SmlI C'TyrA_G
Cuts at: 0 153 1117 1470
Size: 153 964 353
Fragments arranged by size:
964 353 153
SrfI GCCC'GGGC
Cuts at: 0 1449 1470
Size: 1449 21
StuI AGG'CCT
Cuts at: 0 770 1470
Size: 770 700
StyI C'CwwG_G
Cuts at: 0 488 1470
Size: 488 982
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 1470
Size: 577 893
XmaI C'CCGG_G
Cuts at: 0 579 1080 1447 1470
Size: 579 501 367 23
Fragments arranged by size:
579 501 367 23
XmnI GAAnn'nnTTC
Cuts at: 0 1243 1470
Size: 1243 227
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI
BsrFI BtgI EaeI EagI EarI EciI Eco57MI EcoO109I
FspI HaeII HindIII KasI MmeI MslI MspA1I NaeI
NarI NgoMIV NotI PflMI PfoI PmlI PpiI PspOMI
PstI PvuII SacII SapI SfcI SfoI SgrAI SmaI
SmlI SrfI StuI StyI TaqII XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglI BglII BmrI
BmtI BsaI BsaBI BsaWI BsaXI BsmI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NruI NsiI NspI PacI PasI
PciI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI
SgfI SnaBI SpeI SphI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI ZraI