Restriction map for
pF1KB9860
(Linear) (Six-base) MAPSORT of: KIBB9860.seq Check: 1851 from: 1 to: 1218
KIBB9860.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 1218
Size: 1133 85
AfeI AGC'GCT
Cuts at: 0 424 1218
Size: 424 794
AleI CACnn'nnGTG
Cuts at: 0 240 1218
Size: 240 978
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 1218
Size: 1177 32 9
Fragments arranged by size:
1177 32 9
ApaI G_GGCC'C
Cuts at: 0 185 272 1218
Size: 185 87 946
Fragments arranged by size:
946 185 87
ApoI r'AATT_y
Cuts at: 0 301 1066 1218
Size: 301 765 152
Fragments arranged by size:
765 301 152
AvaI C'yCGr_G
Cuts at: 0 126 216 1218
Size: 126 90 1002
Fragments arranged by size:
1002 126 90
BanI G'GyrC_C
Cuts at: 0 122 275 1218
Size: 122 153 943
Fragments arranged by size:
943 153 122
BanII G_rGCy'C
Cuts at: 0 100 185 272 484 1218
Size: 100 85 87 212 734
Fragments arranged by size:
734 212 100 87 85
BbeI G_GCGC'C
Cuts at: 0 126 279 1218
Size: 126 153 939
Fragments arranged by size:
939 153 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 1218
Size: 32 1186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 341 1218
Size: 307 34 877
Fragments arranged by size:
877 307 34
BglI GCCn_nnn'nGGC
Cuts at: 0 437 1218
Size: 437 781
BglII A'GATC_T
Cuts at: 0 587 1218
Size: 587 631
Bme1580I G_kGCm'C
Cuts at: 0 81 185 272 1218
Size: 81 104 87 946
Fragments arranged by size:
946 104 87 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 1218
Size: 261 957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 272 393 1060 1218
Size: 119 153 121 667 158
Fragments arranged by size:
667 158 153 121 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 1218
Size: 114 1104
BsaHI Gr'CG_yC
Cuts at: 0 123 276 1218
Size: 123 153 942
Fragments arranged by size:
942 153 123
BsaWI w'CCGG_w
Cuts at: 0 691 1218
Size: 691 527
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 1218
Size: 73 30 1115
Fragments arranged by size:
1115 73 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 850 853 874 877 919 943 964 1000
Size: 850 3 21 3 42 24 21 36
Cuts at: 1000 1009 1012 1024 1027 1045 1057 1218
Size: 9 3 12 3 18 12 161
Fragments arranged by size:
850 161 42 36 24 21 21 18
12 12 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 74 260 395 425 678 1218
Size: 74 186 135 30 253 540
Fragments arranged by size:
540 253 186 135 74 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1125 1218
Size: 781 344 93
Fragments arranged by size:
781 344 93
BsiEI CG_ry'CG
Cuts at: 0 514 1218
Size: 514 704
BsiHKAI G_wGCw'C
Cuts at: 0 100 135 247 502 1218
Size: 100 35 112 255 716
Fragments arranged by size:
716 255 112 100 35
BsmI GAATG_Cn'
Cuts at: 0 778 1218
Size: 778 440
Bsp1286I G_dGCh'C
Cuts at: 0 81 100 135 185 247 272 484 502
Size: 81 19 35 50 62 25 212 18
Cuts at: 502 1218
Size: 716
Fragments arranged by size:
716 212 81 62 50 35 25 19
18
BspEI T'CCGG_A
Cuts at: 0 691 1218
Size: 691 527
BspHI T'CATG_A
Cuts at: 0 578 1218
Size: 578 640
BsrBI CCG'CTC
Cuts at: 0 377 665 1218
Size: 377 288 553
Fragments arranged by size:
553 377 288
BsrFI r'CCGG_y
Cuts at: 0 513 1218
Size: 513 705
BstXI CCAn_nnnn'nTGG
Cuts at: 0 296 1218
Size: 296 922
BstYI r'GATC_y
Cuts at: 0 587 685 1218
Size: 587 98 533
Fragments arranged by size:
587 533 98
BtgI C'CryG_G
Cuts at: 0 441 475 508 656 1218
Size: 441 34 33 148 562
Fragments arranged by size:
562 441 148 34 33
DraI TTT'AAA
Cuts at: 0 1151 1218
Size: 1151 67
EaeI y'GGCC_r
Cuts at: 0 511 1218
Size: 511 707
EagI C'GGCC_G
Cuts at: 0 511 1218
Size: 511 707
EarI CTCTTCn'nnn_
Cuts at: 0 827 908 929 953 962 1001 1016 1022
Size: 827 81 21 24 9 39 15 6
Cuts at: 1022 1218
Size: 196
Fragments arranged by size:
827 196 81 39 24 21 15 9
6
EcoICRI GAG'CTC
Cuts at: 0 98 1218
Size: 98 1120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 272 393 1060 1133 1218
Size: 119 153 121 667 73 85
Fragments arranged by size:
667 153 121 119 85 73
EcoO109I rG'GnC_Cy
Cuts at: 0 182 268 269 380 1035 1218
Size: 182 86 1 111 655 183
Fragments arranged by size:
655 183 182 111 86 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1160 1218
Size: 1160 58
HaeII r_GCGC'y
Cuts at: 0 126 279 426 556 640 1218
Size: 126 153 147 130 84 578
Fragments arranged by size:
578 153 147 130 126 84
KasI G'GCGC_C
Cuts at: 0 122 275 1218
Size: 122 153 943
Fragments arranged by size:
943 153 122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1218
Size: 255 963
MslI CAynn'nnrTG
Cuts at: 0 240 1218
Size: 240 978
MspA1I CmG'CkG
Cuts at: 0 443 477 490 510 819 1218
Size: 443 34 13 20 309 399
Fragments arranged by size:
443 399 309 34 20 13
NarI GG'CG_CC
Cuts at: 0 123 276 1218
Size: 123 153 942
Fragments arranged by size:
942 153 123
NspI r_CATG'y
Cuts at: 0 549 1218
Size: 549 669
PfoI T'CCnGG_A
Cuts at: 0 288 382 1037 1218
Size: 288 94 655 181
Fragments arranged by size:
655 288 181 94
PpuMI rG'GwC_Cy
Cuts at: 0 380 1035 1218
Size: 380 655 183
Fragments arranged by size:
655 380 183
PsiI TTA'TAA
Cuts at: 0 1135 1218
Size: 1135 83
PspOMI G'GGCC_C
Cuts at: 0 181 268 1218
Size: 181 87 950
Fragments arranged by size:
950 181 87
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 574 606 1218
Size: 574 32 612
Fragments arranged by size:
612 574 32
PstI C_TGCA'G
Cuts at: 0 740 1218
Size: 740 478
SacI G_AGCT'C
Cuts at: 0 100 1218
Size: 100 1118
SacII CC_GC'GG
Cuts at: 0 444 478 511 1218
Size: 444 34 33 707
Fragments arranged by size:
707 444 34 33
SfcI C'TryA_G
Cuts at: 0 736 1218
Size: 736 482
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 437 1218
Size: 437 781
SfoI GGC'GCC
Cuts at: 0 124 277 1218
Size: 124 153 941
Fragments arranged by size:
941 153 124
SmaI CCC'GGG
Cuts at: 0 128 218 1218
Size: 128 90 1000
Fragments arranged by size:
1000 128 90
SmlI C'TyrA_G
Cuts at: 0 93 1218
Size: 93 1125
SphI G_CATG'C
Cuts at: 0 549 1218
Size: 549 669
SrfI GCCC'GGGC
Cuts at: 0 218 1218
Size: 218 1000
StuI AGG'CCT
Cuts at: 0 673 1218
Size: 673 545
StyI C'CwwG_G
Cuts at: 0 187 1218
Size: 187 1031
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 387 583 1218
Size: 187 200 196 635
Fragments arranged by size:
635 200 196 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 1218
Size: 73 32 1113
Fragments arranged by size:
1113 73 32
XmaI C'CCGG_G
Cuts at: 0 126 216 1218
Size: 126 90 1002
Fragments arranged by size:
1002 126 90
XmnI GAAnn'nnTTC
Cuts at: 0 1067 1218
Size: 1067 151
Enzymes that do cut:
AcuI AfeI AleI AloI ApaI ApoI AvaI BanI
BanII BbeI BbsI BcgI BglI BglII Bme1580I BmrI
BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrFI
BstXI BstYI BtgI DraI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII KasI MmeI MslI MspA1I
NarI NspI PfoI PpuMI PsiI PspOMI PsrI PstI
SacI SacII SfcI SfiI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiWI BsmBI
BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PspXI
PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SspI SwaI TatI
Tth111I XbaI XcmI XhoI ZraI