(Linear) (Six-base) MAPSORT of: KIBB9854.seq  Check: 1960  from: 1  to: 1167



KIBB9854.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AccI GT'mk_AC

Cuts at:      0     321    1167 
   Size:        321     846
 
Acc65I G'GTAC_C

Cuts at:      0    1079    1167 
   Size:       1079      88
 
AclI AA'CG_TT

Cuts at:      0     777    1167 
   Size:        777     390
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     730    1167 
   Size:        704      26     437

  Fragments arranged by size: 

                 704     437      26
 
AleI CACnn'nnGTG

Cuts at:      0    1145    1167 
   Size:       1145      22
 
ApoI r'AATT_y

Cuts at:      0      93     286     465     478     508    1167 
   Size:         93     193     179      13      30     659

  Fragments arranged by size: 

                 659     193     179      93      30      13
 
BamHI G'GATC_C

Cuts at:      0     540    1167 
   Size:        540     627
 
BanI G'GyrC_C

Cuts at:      0    1079    1167 
   Size:       1079      88
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     960    1167 
   Size:        960     207
 
BbvCI CC'TCA_GC

Cuts at:      0    1070    1167 
   Size:       1070      97
 
BclI T'GATC_A

Cuts at:      0     971    1167 
   Size:        971     196
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     467    1167 
   Size:        467     700
 
Bpu10I CC'TnA_GC

Cuts at:      0    1070    1100    1167 
   Size:       1070      30      67

  Fragments arranged by size: 

                1070      67      30
 
BsaAI yAC'GTr

Cuts at:      0     390    1167 
   Size:        390     777
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     947     977    1167 
   Size:        947      30     190

  Fragments arranged by size: 

                 947     190      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    1167 
   Size:       1123      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     790    1167 
   Size:        790     377
 
Bsp1286I G_dGCh'C

Cuts at:      0     790    1167 
   Size:        790     377
 
BspHI T'CATG_A

Cuts at:      0     374     938    1167 
   Size:        374     564     229

  Fragments arranged by size: 

                 564     374     229
 
BsrDI GCAATG_nn'

Cuts at:      0     420     647    1167 
   Size:        420     227     520

  Fragments arranged by size: 

                 520     420     227
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     932    1167 
   Size:        932     235
 
BstYI r'GATC_y

Cuts at:      0     243     540    1167 
   Size:        243     297     627

  Fragments arranged by size: 

                 627     297     243
 
BtsI GCAGTG_nn'

Cuts at:      0    1135    1167 
   Size:       1135      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     680    1167 
   Size:        680     487
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     730    1167 
   Size:        704      26     437

  Fragments arranged by size: 

                 704     437      26
 
EcoRV GAT'ATC

Cuts at:      0     372    1167 
   Size:        372     795
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     947     979    1167 
   Size:        947      32     188

  Fragments arranged by size: 

                 947     188      32
 
KpnI G_GTAC'C

Cuts at:      0    1083    1167 
   Size:       1083      84
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     409    1167 
   Size:        409     758
 
MslI CAynn'nnrTG

Cuts at:      0     979    1145    1167 
   Size:        979     166      22

  Fragments arranged by size: 

                 979     166      22
 
MspA1I CmG'CkG

Cuts at:      0    1069    1167 
   Size:       1069      98
 
NdeI CA'TA_TG

Cuts at:      0     667    1167 
   Size:        667     500
 
NspI r_CATG'y

Cuts at:      0     621    1167 
   Size:        621     546
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      82    1167 
   Size:         82    1085
 
PfoI T'CCnGG_A

Cuts at:      0     632    1167 
   Size:        632     535
 
PsiI TTA'TAA

Cuts at:      0      43    1167 
   Size:         43    1124
 
PstI C_TGCA'G

Cuts at:      0     721    1167 
   Size:        721     446
 
PvuII CAG'CTG

Cuts at:      0    1069    1167 
   Size:       1069      98
 
ScaI AGT'ACT

Cuts at:      0     715     995    1167 
   Size:        715     280     172

  Fragments arranged by size: 

                 715     280     172
 
SfcI C'TryA_G

Cuts at:      0     558     717    1167 
   Size:        558     159     450

  Fragments arranged by size: 

                 558     450     159
 
SnaBI TAC'GTA

Cuts at:      0     390    1167 
   Size:        390     777
 
SspI AAT'ATT

Cuts at:      0    1011    1167 
   Size:       1011     156
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1148    1167 
   Size:       1148      19
 
TatI w'GTAC_w

Cuts at:      0     713     993    1167 
   Size:        713     280     174

  Fragments arranged by size: 

                 713     280     174
 
XbaI T'CTAG_A

Cuts at:      0     312     790    1167 
   Size:        312     478     377

  Fragments arranged by size: 

                 478     377     312
 
XmnI GAAnn'nnTTC

Cuts at:      0      71     287    1167 
   Size:         71     216     880

  Fragments arranged by size: 

                 880     216      71
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AleI     ApoI    BamHI     BanI
     BbsI    BbvCI     BclI     BmrI   Bpu10I    BsaAI    BsaXI     BsgI
  BsiHKAI Bsp1286I    BspHI    BsrDI   BstAPI    BstYI     BtsI     EarI
  Eco57MI    EcoRV    Hin4I     KpnI     MmeI     MslI   MspA1I     NdeI
     NspI    PflMI     PfoI     PsiI     PstI    PvuII     ScaI     SfcI
    SnaBI     SspI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BanII     BbeI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI