Restriction map for
pF1KB9853
(Linear) (Six-base) MAPSORT of: KIBB9853.seq Check: 2827 from: 1 to: 1155
KIBB9853.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 786 1155
Size: 786 369
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1155
Size: 862 293
AlwNI CAG_nnn'CTG
Cuts at: 0 450 1155
Size: 450 705
ApoI r'AATT_y
Cuts at: 0 182 566 663 694 728 929 1011 1094
Size: 182 384 97 31 34 201 82 83
Cuts at: 1094 1155
Size: 61
Fragments arranged by size:
384 201 182 97 83 82 61 34
31
BanI G'GyrC_C
Cuts at: 0 262 1134 1155
Size: 262 872 21
Fragments arranged by size:
872 262 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 290 1155
Size: 256 34 865
Fragments arranged by size:
865 256 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 1155
Size: 21 1134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 718 1155
Size: 685 33 437
Fragments arranged by size:
685 437 33
BsaBI GATnn'nnATC
Cuts at: 0 1040 1155
Size: 1040 115
BsaHI Gr'CG_yC
Cuts at: 0 955 1155
Size: 955 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1155
Size: 500 655
BsmI GAATG_Cn'
Cuts at: 0 911 1155
Size: 911 244
BsmBI CGTCTCn'nnnn_
Cuts at: 0 946 1155
Size: 946 209
BspHI T'CATG_A
Cuts at: 0 834 1155
Size: 834 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 1155
Size: 291 864
BstYI r'GATC_y
Cuts at: 0 22 1155
Size: 22 1133
BstZ17I GTA'TAC
Cuts at: 0 787 1155
Size: 787 368
Bsu36I CC'TnA_GG
Cuts at: 0 1017 1155
Size: 1017 138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 125 160 1155
Size: 125 35 995
Fragments arranged by size:
995 125 35
DraI TTT'AAA
Cuts at: 0 822 1155
Size: 822 333
EarI CTCTTCn'nnn_
Cuts at: 0 374 893 1155
Size: 374 519 262
Fragments arranged by size:
519 374 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 718 862 1155
Size: 685 33 144 293
Fragments arranged by size:
685 293 144 33
EcoRI G'AATT_C
Cuts at: 0 566 1011 1155
Size: 566 445 144
Fragments arranged by size:
566 445 144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 778 1155
Size: 778 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 1155
Size: 103 32 1020
Fragments arranged by size:
1020 103 32
HincII GTy'rAC
Cuts at: 0 171 336 1155
Size: 171 165 819
Fragments arranged by size:
819 171 165
HpaI GTT'AAC
Cuts at: 0 336 1155
Size: 336 819
MfeI C'AATT_G
Cuts at: 0 311 1155
Size: 311 844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1155
Size: 349 806
MspA1I CmG'CkG
Cuts at: 0 331 514 1155
Size: 331 183 641
Fragments arranged by size:
641 331 183
NspI r_CATG'y
Cuts at: 0 95 1155
Size: 95 1060
PfoI T'CCnGG_A
Cuts at: 0 701 1155
Size: 701 454
PstI C_TGCA'G
Cuts at: 0 37 1155
Size: 37 1118
PvuII CAG'CTG
Cuts at: 0 514 1155
Size: 514 641
SbfI CC_TGCA'GG
Cuts at: 0 37 1155
Size: 37 1118
SfcI C'TryA_G
Cuts at: 0 33 278 318 1023 1155
Size: 33 245 40 705 132
Fragments arranged by size:
705 245 132 40 33
SphI G_CATG'C
Cuts at: 0 95 1155
Size: 95 1060
SspI AAT'ATT
Cuts at: 0 246 1155
Size: 246 909
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 355 1155
Size: 355 800
TatI w'GTAC_w
Cuts at: 0 987 992 1155
Size: 987 5 163
Fragments arranged by size:
987 163 5
XbaI T'CTAG_A
Cuts at: 0 585 1155
Size: 585 570
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanI BcgI BmrI BpmI
BsaBI BsaHI BsgI BsmI BsmBI BspHI BstXI BstYI
BstZ17I Bsu36I CspCI DraI EarI Eco57MI EcoRI FalI
Hin4I HincII HpaI MfeI MmeI MspA1I NspI PfoI
PstI PvuII SbfI SfcI SphI SspI TaqII TatI
XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BtgI BtgZI BtsI ClaI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII HindIII KasI KpnI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI StuI StyI SwaI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI