Restriction map for
pF1KB9851
(Linear) (Six-base) MAPSORT of: KIBB9851.seq Check: 4224 from: 1 to: 1140
KIBB9851.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1037 1140
Size: 907 130 103
Fragments arranged by size:
907 130 103
AhdI GACnn_n'nnGTC
Cuts at: 0 873 1140
Size: 873 267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 580 1140
Size: 445 135 560
Fragments arranged by size:
560 445 135
AlwNI CAG_nnn'CTG
Cuts at: 0 126 645 877 1140
Size: 126 519 232 263
Fragments arranged by size:
519 263 232 126
ApaI G_GGCC'C
Cuts at: 0 728 1140
Size: 728 412
ApoI r'AATT_y
Cuts at: 0 5 373 616 1140
Size: 5 368 243 524
Fragments arranged by size:
524 368 243 5
BamHI G'GATC_C
Cuts at: 0 1084 1140
Size: 1084 56
BanII G_rGCy'C
Cuts at: 0 728 1140
Size: 728 412
BbvCI CC'TCA_GC
Cuts at: 0 314 1140
Size: 314 826
BciVI GTATCCnnnnn_n'
Cuts at: 0 721 1140
Size: 721 419
BclI T'GATC_A
Cuts at: 0 917 1140
Size: 917 223
BglI GCCn_nnn'nGGC
Cuts at: 0 443 998 1140
Size: 443 555 142
Fragments arranged by size:
555 443 142
BlpI GC'TnA_GC
Cuts at: 0 433 1058 1140
Size: 433 625 82
Fragments arranged by size:
625 433 82
Bme1580I G_kGCm'C
Cuts at: 0 728 1140
Size: 728 412
BmrI ACTGGGnnnn_n'
Cuts at: 0 38 461 1118 1140
Size: 38 423 657 22
Fragments arranged by size:
657 423 38 22
Bpu10I CC'TnA_GC
Cuts at: 0 314 341 625 1140
Size: 314 27 284 515
Fragments arranged by size:
515 314 284 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1140
Size: 259 881
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 1140
Size: 169 971
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 874 904 1140
Size: 874 30 236
Fragments arranged by size:
874 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 784 967 1140
Size: 181 603 183 173
Fragments arranged by size:
603 183 181 173
BseYI C'CCAG_C
Cuts at: 0 349 645 1140
Size: 349 296 495
Fragments arranged by size:
495 349 296
Bsp1286I G_dGCh'C
Cuts at: 0 728 1140
Size: 728 412
BsrBI CCG'CTC
Cuts at: 0 306 1140
Size: 306 834
BsrFI r'CCGG_y
Cuts at: 0 1039 1140
Size: 1039 101
BstEII G'GTnAC_C
Cuts at: 0 1035 1140
Size: 1035 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 818 1140
Size: 818 322
BstYI r'GATC_y
Cuts at: 0 672 1084 1140
Size: 672 412 56
Fragments arranged by size:
672 412 56
Bsu36I CC'TnA_GG
Cuts at: 0 857 1140
Size: 857 283
BtsI GCAGTG_nn'
Cuts at: 0 306 699 972 1140
Size: 306 393 273 168
Fragments arranged by size:
393 306 273 168
DraI TTT'AAA
Cuts at: 0 1128 1140
Size: 1128 12
DraIII CAC_nnn'GTG
Cuts at: 0 469 1140
Size: 469 671
EaeI y'GGCC_r
Cuts at: 0 17 81 92 290 1140
Size: 17 64 11 198 850
Fragments arranged by size:
850 198 64 17 11
EarI CTCTTCn'nnn_
Cuts at: 0 850 905 1056 1140
Size: 850 55 151 84
Fragments arranged by size:
850 151 84 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1037 1140
Size: 907 130 103
Fragments arranged by size:
907 130 103
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 655 1140
Size: 499 156 485
Fragments arranged by size:
499 485 156
EcoRI G'AATT_C
Cuts at: 0 5 616 1140
Size: 5 611 524
Fragments arranged by size:
611 524 5
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 93 1140
Size: 93 1047
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 859 891 1140
Size: 102 32 725 32 249
Fragments arranged by size:
725 249 102 32 32
MscI TGG'CCA
Cuts at: 0 19 94 292 1140
Size: 19 75 198 848
Fragments arranged by size:
848 198 75 19
MslI CAynn'nnrTG
Cuts at: 0 217 413 1140
Size: 217 196 727
Fragments arranged by size:
727 217 196
MspA1I CmG'CkG
Cuts at: 0 330 485 1140
Size: 330 155 655
Fragments arranged by size:
655 330 155
NspI r_CATG'y
Cuts at: 0 201 1140
Size: 201 939
PasI CC'CwG_GG
Cuts at: 0 1008 1140
Size: 1008 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 1140
Size: 192 948
PfoI T'CCnGG_A
Cuts at: 0 53 611 1140
Size: 53 558 529
Fragments arranged by size:
558 529 53
PspOMI G'GGCC_C
Cuts at: 0 724 1140
Size: 724 416
PstI C_TGCA'G
Cuts at: 0 161 335 641 1140
Size: 161 174 306 499
Fragments arranged by size:
499 306 174 161
PvuII CAG'CTG
Cuts at: 0 330 485 1140
Size: 330 155 655
Fragments arranged by size:
655 330 155
SapI GCTCTTCn'nnn_
Cuts at: 0 850 905 1140
Size: 850 55 235
Fragments arranged by size:
850 235 55
SfcI C'TryA_G
Cuts at: 0 157 331 637 1140
Size: 157 174 306 503
Fragments arranged by size:
503 306 174 157
SmlI C'TyrA_G
Cuts at: 0 274 1140
Size: 274 866
StuI AGG'CCT
Cuts at: 0 14 1140
Size: 14 1126
StyI C'CwwG_G
Cuts at: 0 996 1079 1140
Size: 996 83 61
Fragments arranged by size:
996 83 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 480 1021 1140
Size: 480 541 119
Fragments arranged by size:
541 480 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 874 906 1140
Size: 874 32 234
Fragments arranged by size:
874 234 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 219 735 1140
Size: 189 30 516 405
Fragments arranged by size:
516 405 189 30
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApaI ApoI BamHI BanII
BbvCI BciVI BclI BglI BlpI Bme1580I BmrI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI Bsp1286I BsrBI BsrFI
BstEII BstXI BstYI Bsu36I BtsI DraI DraIII EaeI
EarI Eco57MI EcoNI EcoRI FalI Hin4I MscI MslI
MspA1I NspI PasI PflMI PfoI PspOMI PstI PvuII
SapI SfcI SmlI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BcgI BfrBI BglII
BmgBI BmtI BplI BpmI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgI BtgZI ClaI CspCI DrdI EagI EciI EcoICRI
EcoO109I EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI