Restriction map for
pF1KB9844
(Linear) (Six-base) MAPSORT of: KIBB9844.seq Check: 5153 from: 1 to: 1116
KIBB9844.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AleI CACnn'nnGTG
Cuts at: 0 851 1116
Size: 851 265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1009 1116
Size: 1009 107
ApaI G_GGCC'C
Cuts at: 0 839 1116
Size: 839 277
ApaLI G'TGCA_C
Cuts at: 0 936 1116
Size: 936 180
ApoI r'AATT_y
Cuts at: 0 709 1116
Size: 709 407
BanII G_rGCy'C
Cuts at: 0 65 86 839 886 1116
Size: 65 21 753 47 230
Fragments arranged by size:
753 230 65 47 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 341 1105 1116
Size: 341 764 11
Fragments arranged by size:
764 341 11
BglI GCCn_nnn'nGGC
Cuts at: 0 379 469 1116
Size: 379 90 647
Fragments arranged by size:
647 379 90
Bme1580I G_kGCm'C
Cuts at: 0 496 839 940 1030 1116
Size: 496 343 101 90 86
Fragments arranged by size:
496 343 101 90 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 468 556 1116
Size: 58 410 88 560
Fragments arranged by size:
560 410 88 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1116
Size: 898 218
BsaAI yAC'GTr
Cuts at: 0 849 1116
Size: 849 267
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 529 559 1116
Size: 295 30 204 30 557
Fragments arranged by size:
557 295 204 30 30
BseYI C'CCAG_C
Cuts at: 0 421 433 1116
Size: 421 12 683
Fragments arranged by size:
683 421 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1116
Size: 875 241
BsiHKAI G_wGCw'C
Cuts at: 0 65 344 886 940 1116
Size: 65 279 542 54 176
Fragments arranged by size:
542 279 176 65 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 888 1116
Size: 888 228
Bsp1286I G_dGCh'C
Cuts at: 0 65 86 344 496 839 886 940 1030
Size: 65 21 258 152 343 47 54 90
Cuts at: 1030 1116
Size: 86
Fragments arranged by size:
343 258 152 90 86 65 54 47
21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 513 1116
Size: 513 603
BsrBI CCG'CTC
Cuts at: 0 192 1116
Size: 192 924
BsrFI r'CCGG_y
Cuts at: 0 916 1006 1116
Size: 916 90 110
Fragments arranged by size:
916 110 90
BssSI C'ACGA_G
Cuts at: 0 495 1116
Size: 495 621
BstBI TT'CG_AA
Cuts at: 0 944 1116
Size: 944 172
BstEII G'GTnAC_C
Cuts at: 0 668 1116
Size: 668 448
BstYI r'GATC_y
Cuts at: 0 41 146 690 1116
Size: 41 105 544 426
Fragments arranged by size:
544 426 105 41
BtgI C'CryG_G
Cuts at: 0 831 1116
Size: 831 285
BtsI GCAGTG_nn'
Cuts at: 0 599 1116
Size: 599 517
DrdI GACnn_nn'nnGTC
Cuts at: 0 561 1116
Size: 561 555
EaeI y'GGCC_r
Cuts at: 0 156 1116
Size: 156 960
EarI CTCTTCn'nnn_
Cuts at: 0 199 1116
Size: 199 917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 158 1116
Size: 158 958
EcoICRI GAG'CTC
Cuts at: 0 63 884 1116
Size: 63 821 232
Fragments arranged by size:
821 232 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 468 556 1116
Size: 58 410 88 560
Fragments arranged by size:
560 410 88 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 892 1116
Size: 892 224
FspI TGC'GCA
Cuts at: 0 985 997 1116
Size: 985 12 119
Fragments arranged by size:
985 119 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 804 836 1116
Size: 804 32 280
Fragments arranged by size:
804 280 32
HincII GTy'rAC
Cuts at: 0 1064 1116
Size: 1064 52
HindIII A'AGCT_T
Cuts at: 0 869 1116
Size: 869 247
MfeI C'AATT_G
Cuts at: 0 141 1116
Size: 141 975
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 130 1116
Size: 32 98 986
Fragments arranged by size:
986 98 32
MscI TGG'CCA
Cuts at: 0 158 1116
Size: 158 958
MslI CAynn'nnrTG
Cuts at: 0 76 851 1116
Size: 76 775 265
Fragments arranged by size:
775 265 76
MspA1I CmG'CkG
Cuts at: 0 437 702 906 1116
Size: 437 265 204 210
Fragments arranged by size:
437 265 210 204
NspI r_CATG'y
Cuts at: 0 1096 1116
Size: 1096 20
PfoI T'CCnGG_A
Cuts at: 0 320 794 1116
Size: 320 474 322
Fragments arranged by size:
474 322 320
PspOMI G'GGCC_C
Cuts at: 0 835 1116
Size: 835 281
PvuII CAG'CTG
Cuts at: 0 702 906 1116
Size: 702 204 210
Fragments arranged by size:
702 210 204
SacI G_AGCT'C
Cuts at: 0 65 886 1116
Size: 65 821 230
Fragments arranged by size:
821 230 65
SapI GCTCTTCn'nnn_
Cuts at: 0 199 1116
Size: 199 917
ScaI AGT'ACT
Cuts at: 0 637 1116
Size: 637 479
SgrAI Cr'CCGG_yG
Cuts at: 0 916 1006 1116
Size: 916 90 110
Fragments arranged by size:
916 110 90
SmlI C'TyrA_G
Cuts at: 0 877 1116
Size: 877 239
StyI C'CwwG_G
Cuts at: 0 585 1116
Size: 585 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 586 1116
Size: 586 530
TatI w'GTAC_w
Cuts at: 0 635 1116
Size: 635 481
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 1116
Size: 138 32 946
Fragments arranged by size:
946 138 32
Enzymes that do cut:
AleI AlfI ApaI ApaLI ApoI BanII BbsI BglI
Bme1580I BpmI BpuEI BsaAI BsaXI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstBI BstEII
BstYI BtgI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI FalI FspI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NspI PfoI PspOMI PvuII SacI
SapI ScaI SgrAI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI AlwNI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstAPI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfcI SfiI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI