(Linear) (Six-base) MAPSORT of: KIBB9839.seq  Check: 4647  from: 1  to: 1065



KIBB9839.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AflIII A'CryG_T

Cuts at:      0     359    1065 
   Size:        359     706
 
AlwNI CAG_nnn'CTG

Cuts at:      0     958    1065 
   Size:        958     107
 
ApoI r'AATT_y

Cuts at:      0      31     155     297     628    1065 
   Size:         31     124     142     331     437

  Fragments arranged by size: 

                 437     331     142     124      31
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     955     988    1065 
   Size:        955      33      77

  Fragments arranged by size: 

                 955      77      33
 
BanI G'GyrC_C

Cuts at:      0     605    1065 
   Size:        605     460
 
BanII G_rGCy'C

Cuts at:      0     809    1065 
   Size:        809     256
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211    1065 
   Size:        211     854
 
BclI T'GATC_A

Cuts at:      0     389     599    1065 
   Size:        389     210     466

  Fragments arranged by size: 

                 466     389     210
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     123     345    1065 
   Size:        123     222     720

  Fragments arranged by size: 

                 720     222     123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     705    1065 
   Size:         91     614     360

  Fragments arranged by size: 

                 614     360      91
 
Bpu10I CC'TnA_GC

Cuts at:      0    1018    1065 
   Size:       1018      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     952    1065 
   Size:        436     516     113

  Fragments arranged by size: 

                 516     436     113
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     123    1065 
   Size:        123     942
 
BsaWI w'CCGG_w

Cuts at:      0     944    1065 
   Size:        944     121
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133    1065 
   Size:        103      30     932

  Fragments arranged by size: 

                 932     103      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100    1065 
   Size:        100     965
 
BseYI C'CCAG_C

Cuts at:      0      99    1065 
   Size:         99     966
 
BsiHKAI G_wGCw'C

Cuts at:      0     809    1065 
   Size:        809     256
 
BsmI GAATG_Cn'

Cuts at:      0     877    1065 
   Size:        877     188
 
Bsp1286I G_dGCh'C

Cuts at:      0     809    1065 
   Size:        809     256
 
BsrDI GCAATG_nn'

Cuts at:      0     239    1065 
   Size:        239     826
 
BsrGI T'GTAC_A

Cuts at:      0     275    1065 
   Size:        275     790
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     873    1065 
   Size:        873     192
 
BtsI GCAGTG_nn'

Cuts at:      0     344    1065 
   Size:        344     721
 
DraIII CAC_nnn'GTG

Cuts at:      0     221     840     980    1065 
   Size:        221     619     140      85

  Fragments arranged by size: 

                 619     221     140      85
 
EaeI y'GGCC_r

Cuts at:      0     290    1065 
   Size:        290     775
 
EarI CTCTTCn'nnn_

Cuts at:      0     652    1065 
   Size:        652     413
 
EcoICRI GAG'CTC

Cuts at:      0     807    1065 
   Size:        807     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     705    1065 
   Size:         91     614     360

  Fragments arranged by size: 

                 614     360      91
 
EcoRI G'AATT_C

Cuts at:      0     155    1065 
   Size:        155     910
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     768     800    1065 
   Size:         49      32     687      32     265

  Fragments arranged by size: 

                 687     265      49      32      32
 
MscI TGG'CCA

Cuts at:      0     292    1065 
   Size:        292     773
 
MspA1I CmG'CkG

Cuts at:      0      99     992    1065 
   Size:         99     893      73

  Fragments arranged by size: 

                 893      99      73
 
NspI r_CATG'y

Cuts at:      0     363    1065 
   Size:        363     702
 
PciI A'CATG_T

Cuts at:      0     359    1065 
   Size:        359     706
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     221    1065 
   Size:        221     844
 
PfoI T'CCnGG_A

Cuts at:      0     159     524    1049    1065 
   Size:        159     365     525      16

  Fragments arranged by size: 

                 525     365     159      16
 
PvuII CAG'CTG

Cuts at:      0      99     992    1065 
   Size:         99     893      73

  Fragments arranged by size: 

                 893      99      73
 
SacI G_AGCT'C

Cuts at:      0     809    1065 
   Size:        809     256
 
SmlI C'TyrA_G

Cuts at:      0     451     967    1065 
   Size:        451     516      98

  Fragments arranged by size: 

                 516     451      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     612    1065 
   Size:        612     453
 
TatI w'GTAC_w

Cuts at:      0     275     518     971    1065 
   Size:        275     243     453      94

  Fragments arranged by size: 

                 453     275     243      94
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     103     135    1065 
   Size:        103      32     930

  Fragments arranged by size: 

                 930     103      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     310    1065 
   Size:        310     755
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     BaeI     BanI    BanII     BbsI     BclI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI    BstXI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoRI    Hin4I     MscI
   MspA1I     NspI     PciI    PflMI     PfoI    PvuII     SacI     SmlI
    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI