(Linear) (Six-base) MAPSORT of: KIBB9837.seq  Check: 6455  from: 1  to: 1056



KIBB9837.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     538     828    1056 
   Size:        538     290     228

  Fragments arranged by size: 

                 538     290     228
 
Acc65I G'GTAC_C

Cuts at:      0     377    1056 
   Size:        377     679
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1056 
   Size:        202     854
 
AleI CACnn'nnGTG

Cuts at:      0     457    1056 
   Size:        457     599
 
ApoI r'AATT_y

Cuts at:      0     573     784    1056 
   Size:        573     211     272

  Fragments arranged by size: 

                 573     272     211
 
AvaI C'yCGr_G

Cuts at:      0     642     994    1056 
   Size:        642     352      62

  Fragments arranged by size: 

                 642     352      62
 
BanI G'GyrC_C

Cuts at:      0     377    1056 
   Size:        377     679
 
BanII G_rGCy'C

Cuts at:      0      56     261     320     747    1056 
   Size:         56     205      59     427     309

  Fragments arranged by size: 

                 427     309     205      59      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     511     921    1056 
   Size:        511     410     135

  Fragments arranged by size: 

                 511     410     135
 
BclI T'GATC_A

Cuts at:      0     449    1056 
   Size:        449     607
 
BglII A'GATC_T

Cuts at:      0     502    1056 
   Size:        502     554
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     270    1037    1056 
   Size:        270     767      19

  Fragments arranged by size: 

                 767     270      19
 
BsaBI GATnn'nnATC

Cuts at:      0     595    1056 
   Size:        595     461
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     482    1056 
   Size:        482     574
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     261     320     747    1056 
   Size:         56     205      59     427     309

  Fragments arranged by size: 

                 427     309     205      59      56
 
BspHI T'CATG_A

Cuts at:      0     719    1056 
   Size:        719     337
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     212     538     828    1056 
   Size:        212     326     290     228

  Fragments arranged by size: 

                 326     290     228     212
 
BsrBI CCG'CTC

Cuts at:      0     105    1056 
   Size:        105     951
 
BsrDI GCAATG_nn'

Cuts at:      0     944    1056 
   Size:        944     112
 
BstEII G'GTnAC_C

Cuts at:      0     423    1056 
   Size:        423     633
 
BstYI r'GATC_y

Cuts at:      0     502    1056 
   Size:        502     554
 
BtsI GCAGTG_nn'

Cuts at:      0     864    1056 
   Size:        864     192
 
ClaI AT'CG_AT

Cuts at:      0     371    1056 
   Size:        371     685
 
DraI TTT'AAA

Cuts at:      0     410    1056 
   Size:        410     646
 
EaeI y'GGCC_r

Cuts at:      0     215    1056 
   Size:        215     841
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     679     742    1056 
   Size:        178     501      63     314

  Fragments arranged by size: 

                 501     314     178      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1056 
   Size:        202     854
 
EcoRI G'AATT_C

Cuts at:      0     784    1056 
   Size:        784     272
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     535    1056 
   Size:        535     521
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     834     866    1056 
   Size:        834      32     190

  Fragments arranged by size: 

                 834     190      32
 
HincII GTy'rAC

Cuts at:      0     304    1056 
   Size:        304     752
 
KpnI G_GTAC'C

Cuts at:      0     381    1056 
   Size:        381     675
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1056 
   Size:        803     253
 
MscI TGG'CCA

Cuts at:      0     217    1056 
   Size:        217     839
 
MslI CAynn'nnrTG

Cuts at:      0     457     718    1056 
   Size:        457     261     338

  Fragments arranged by size: 

                 457     338     261
 
MspA1I CmG'CkG

Cuts at:      0     618    1056 
   Size:        618     438
 
NspI r_CATG'y

Cuts at:      0     147    1056 
   Size:        147     909
 
PfoI T'CCnGG_A

Cuts at:      0     779    1056 
   Size:        779     277
 
PstI C_TGCA'G

Cuts at:      0     535    1056 
   Size:        535     521
 
SapI GCTCTTCn'nnn_

Cuts at:      0     178    1056 
   Size:        178     878
 
SfcI C'TryA_G

Cuts at:      0     531    1056 
   Size:        531     525
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     447    1056 
   Size:        447     609
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     283     787    1056 
   Size:        283     504     269

  Fragments arranged by size: 

                 504     283     269
 
XmnI GAAnn'nnTTC

Cuts at:      0     518     574     775    1056 
   Size:        518      56     201     281

  Fragments arranged by size: 

                 518     281     201      56
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI     ApoI     AvaI     BanI    BanII
     BbsI     BclI    BglII     BmrI    BsaBI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI   BstEII    BstYI     BtsI     ClaI     DraI
     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII     KpnI
     MmeI     MscI     MslI   MspA1I     NspI     PfoI     PstI     SapI
     SfcI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI