Restriction map for
pF1KB9836
(Linear) (Six-base) MAPSORT of: KIBB9836.seq Check: 8469 from: 1 to: 1053
KIBB9836.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 576 1053
Size: 576 477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1053
Size: 442 611
AfeI AGC'GCT
Cuts at: 0 59 1053
Size: 59 994
AlwNI CAG_nnn'CTG
Cuts at: 0 834 958 1053
Size: 834 124 95
Fragments arranged by size:
834 124 95
ApaI G_GGCC'C
Cuts at: 0 429 1053
Size: 429 624
ApoI r'AATT_y
Cuts at: 0 322 538 1053
Size: 322 216 515
Fragments arranged by size:
515 322 216
AvaI C'yCGr_G
Cuts at: 0 504 1053
Size: 504 549
AvrII C'CTAG_G
Cuts at: 0 739 1053
Size: 739 314
BanI G'GyrC_C
Cuts at: 0 588 1053
Size: 588 465
BanII G_rGCy'C
Cuts at: 0 369 429 1053
Size: 369 60 624
Fragments arranged by size:
624 369 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 1053
Size: 26 1027
BbvCI CC'TCA_GC
Cuts at: 0 1004 1053
Size: 1004 49
BglI GCCn_nnn'nGGC
Cuts at: 0 632 1053
Size: 632 421
Bme1580I G_kGCm'C
Cuts at: 0 429 1053
Size: 429 624
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 383 1053
Size: 383 670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 503 835 1009 1053
Size: 285 218 332 174 44
Fragments arranged by size:
332 285 218 174 44
Bpu10I CC'TnA_GC
Cuts at: 0 1004 1053
Size: 1004 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 730 1053
Size: 646 84 323
Fragments arranged by size:
646 323 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 808 838 1053
Size: 808 30 215
Fragments arranged by size:
808 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 727 860 1021 1024 1027 1053
Size: 121 606 133 161 3 3 26
Fragments arranged by size:
606 161 133 121 26 3 3
BseYI C'CCAG_C
Cuts at: 0 636 898 951 1053
Size: 636 262 53 102
Fragments arranged by size:
636 262 102 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1053
Size: 48 1005
BsiHKAI G_wGCw'C
Cuts at: 0 369 1053
Size: 369 684
Bsp1286I G_dGCh'C
Cuts at: 0 369 429 1053
Size: 369 60 624
Fragments arranged by size:
624 369 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 576 741 1053
Size: 576 165 312
Fragments arranged by size:
576 312 165
BsrDI GCAATG_nn'
Cuts at: 0 478 893 1053
Size: 478 415 160
Fragments arranged by size:
478 415 160
BsrFI r'CCGG_y
Cuts at: 0 70 1053
Size: 70 983
Bsu36I CC'TnA_GG
Cuts at: 0 236 1053
Size: 236 817
BtsI GCAGTG_nn'
Cuts at: 0 96 1053
Size: 96 957
EarI CTCTTCn'nnn_
Cuts at: 0 461 598 1053
Size: 461 137 455
Fragments arranged by size:
461 455 137
EcoICRI GAG'CTC
Cuts at: 0 367 1053
Size: 367 686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 442 503 835 1009 1053
Size: 285 157 61 332 174 44
Fragments arranged by size:
332 285 174 157 61 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1024 1053
Size: 1024 29
EcoO109I rG'GnC_Cy
Cuts at: 0 426 1017 1053
Size: 426 591 36
Fragments arranged by size:
591 426 36
HaeII r_GCGC'y
Cuts at: 0 61 1053
Size: 61 992
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 657 689 1053
Size: 657 32 364
Fragments arranged by size:
657 364 32
MfeI C'AATT_G
Cuts at: 0 851 1053
Size: 851 202
MslI CAynn'nnrTG
Cuts at: 0 346 380 1053
Size: 346 34 673
Fragments arranged by size:
673 346 34
MspA1I CmG'CkG
Cuts at: 0 1003 1053
Size: 1003 50
NaeI GCC'GGC
Cuts at: 0 72 1053
Size: 72 981
NgoMIV G'CCGG_C
Cuts at: 0 70 1053
Size: 70 983
PflMI CCAn_nnn'nTGG
Cuts at: 0 946 958 1053
Size: 946 12 95
Fragments arranged by size:
946 95 12
PfoI T'CCnGG_A
Cuts at: 0 413 1053
Size: 413 640
PspOMI G'GGCC_C
Cuts at: 0 425 1053
Size: 425 628
PstI C_TGCA'G
Cuts at: 0 91 119 704 1053
Size: 91 28 585 349
Fragments arranged by size:
585 349 91 28
PvuII CAG'CTG
Cuts at: 0 1003 1053
Size: 1003 50
SacI G_AGCT'C
Cuts at: 0 369 1053
Size: 369 684
SapI GCTCTTCn'nnn_
Cuts at: 0 461 1053
Size: 461 592
SfcI C'TryA_G
Cuts at: 0 87 115 700 1053
Size: 87 28 585 353
Fragments arranged by size:
585 353 87 28
SmlI C'TyrA_G
Cuts at: 0 661 709 1053
Size: 661 48 344
Fragments arranged by size:
661 344 48
SpeI A'CTAG_T
Cuts at: 0 217 1053
Size: 217 836
SspI AAT'ATT
Cuts at: 0 1034 1053
Size: 1034 19
StyI C'CwwG_G
Cuts at: 0 134 739 1053
Size: 134 605 314
Fragments arranged by size:
605 314 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 340 1053
Size: 340 713
TatI w'GTAC_w
Cuts at: 0 143 804 1053
Size: 143 661 249
Fragments arranged by size:
661 249 143
Tth111I GACn'n_nGTC
Cuts at: 0 889 1053
Size: 889 164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 895 1053
Size: 895 158
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI ApoI AvaI AvrII
BanI BanII BbsI BbvCI BglI Bme1580I BplI BpmI
Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI Bsu36I BtsI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I MfeI MslI MspA1I NaeI
NgoMIV PflMI PfoI PspOMI PstI PvuII SacI SapI
SfcI SmlI SpeI SspI StyI TaqII TatI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI StuI SwaI TstI XbaI XhoI
XmaI XmnI ZraI