Restriction map for
pF1KB9830
(Linear) (Six-base) MAPSORT of: KIBB9830.seq Check: 865 from: 1 to: 1026
KIBB9830.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 11 1026
Size: 11 1015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 958 1026
Size: 897 61 68
Fragments arranged by size:
897 68 61
AflII C'TTAA_G
Cuts at: 0 187 1026
Size: 187 839
AgeI A'CCGG_T
Cuts at: 0 650 1026
Size: 650 376
ApaLI G'TGCA_C
Cuts at: 0 48 1026
Size: 48 978
ApoI r'AATT_y
Cuts at: 0 239 767 918 1026
Size: 239 528 151 108
Fragments arranged by size:
528 239 151 108
AseI AT'TA_AT
Cuts at: 0 599 1026
Size: 599 427
AvaI C'yCGr_G
Cuts at: 0 791 1026
Size: 791 235
BamHI G'GATC_C
Cuts at: 0 879 1026
Size: 879 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 379 1026
Size: 379 647
BfrBI ATG'CAT
Cuts at: 0 871 1026
Size: 871 155
Bme1580I G_kGCm'C
Cuts at: 0 52 946 1026
Size: 52 894 80
Fragments arranged by size:
894 80 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 1026
Size: 268 758
Bpu10I CC'TnA_GC
Cuts at: 0 781 1026
Size: 781 245
BsaAI yAC'GTr
Cuts at: 0 660 1026
Size: 660 366
BsaBI GATnn'nnATC
Cuts at: 0 355 1026
Size: 355 671
BsaWI w'CCGG_w
Cuts at: 0 158 650 1026
Size: 158 492 376
Fragments arranged by size:
492 376 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 1026
Size: 792 234
BsiEI CG_ry'CG
Cuts at: 0 363 1026
Size: 363 663
BsiHKAI G_wGCw'C
Cuts at: 0 52 847 1026
Size: 52 795 179
Fragments arranged by size:
795 179 52
BsmI GAATG_Cn'
Cuts at: 0 448 1026
Size: 448 578
Bsp1286I G_dGCh'C
Cuts at: 0 52 847 946 1026
Size: 52 795 99 80
Fragments arranged by size:
795 99 80 52
BspEI T'CCGG_A
Cuts at: 0 158 1026
Size: 158 868
BspHI T'CATG_A
Cuts at: 0 215 1026
Size: 215 811
BsrDI GCAATG_nn'
Cuts at: 0 722 1026
Size: 722 304
BsrFI r'CCGG_y
Cuts at: 0 650 1026
Size: 650 376
BstEII G'GTnAC_C
Cuts at: 0 1000 1026
Size: 1000 26
BstYI r'GATC_y
Cuts at: 0 879 1026
Size: 879 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 151 1026
Size: 151 875
BtsI GCAGTG_nn'
Cuts at: 0 85 809 1026
Size: 85 724 217
Fragments arranged by size:
724 217 85
ClaI AT'CG_AT
Cuts at: 0 359 1026
Size: 359 667
DraI TTT'AAA
Cuts at: 0 390 1026
Size: 390 636
DraIII CAC_nnn'GTG
Cuts at: 0 851 1026
Size: 851 175
EarI CTCTTCn'nnn_
Cuts at: 0 85 1007 1026
Size: 85 922 19
Fragments arranged by size:
922 85 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 958 1026
Size: 897 61 68
Fragments arranged by size:
897 68 61
EcoO109I rG'GnC_Cy
Cuts at: 0 75 1026
Size: 75 951
EcoRI G'AATT_C
Cuts at: 0 767 1026
Size: 767 259
HincII GTy'rAC
Cuts at: 0 12 857 1026
Size: 12 845 169
Fragments arranged by size:
845 169 12
HpaI GTT'AAC
Cuts at: 0 857 1026
Size: 857 169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 422 1026
Size: 93 329 604
Fragments arranged by size:
604 329 93
NsiI A_TGCA'T
Cuts at: 0 873 1026
Size: 873 153
NspI r_CATG'y
Cuts at: 0 875 1026
Size: 875 151
PpuMI rG'GwC_Cy
Cuts at: 0 75 1026
Size: 75 951
PvuI CG_AT'CG
Cuts at: 0 363 1026
Size: 363 663
SalI G'TCGA_C
Cuts at: 0 10 1026
Size: 10 1016
SmaI CCC'GGG
Cuts at: 0 793 1026
Size: 793 233
SmlI C'TyrA_G
Cuts at: 0 187 1026
Size: 187 839
SnaBI TAC'GTA
Cuts at: 0 660 1026
Size: 660 366
SphI G_CATG'C
Cuts at: 0 875 1026
Size: 875 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 535 1026
Size: 85 450 491
Fragments arranged by size:
491 450 85
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 1026
Size: 411 615
XmaI C'CCGG_G
Cuts at: 0 791 1026
Size: 791 235
Enzymes that do cut:
AccI AcuI AflII AgeI ApaLI ApoI AseI AvaI
BamHI BbsI BfrBI Bme1580I BmrI Bpu10I BsaAI BsaBI
BsaWI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BsrDI BsrFI BstEII BstYI BtgZI BtsI ClaI DraI
DraIII EarI Eco57MI EcoO109I EcoRI HincII HpaI MmeI
NsiI NspI PpuMI PvuI SalI SmaI SmlI SnaBI
SphI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI AscI AsiSI AvrII BaeI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsiWI BsmBI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgI CspCI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII
KasI KpnI MfeI MluI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SpeI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI