Restriction map for
pF1KB9828
(Linear) (Six-base) MAPSORT of: KIBB9828.seq Check: 9331 from: 1 to: 990
KIBB9828.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 855 990
Size: 855 135
Acc65I G'GTAC_C
Cuts at: 0 224 990
Size: 224 766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 990
Size: 815 175
AfeI AGC'GCT
Cuts at: 0 319 990
Size: 319 671
AflIII A'CryG_T
Cuts at: 0 716 990
Size: 716 274
AleI CACnn'nnGTG
Cuts at: 0 427 990
Size: 427 563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 954 986 990
Size: 954 32 4
Fragments arranged by size:
954 32 4
AlwNI CAG_nnn'CTG
Cuts at: 0 870 990
Size: 870 120
ApaI G_GGCC'C
Cuts at: 0 642 990
Size: 642 348
ApaLI G'TGCA_C
Cuts at: 0 130 990
Size: 130 860
BamHI G'GATC_C
Cuts at: 0 104 990
Size: 104 886
BanI G'GyrC_C
Cuts at: 0 224 650 990
Size: 224 426 340
Fragments arranged by size:
426 340 224
BanII G_rGCy'C
Cuts at: 0 22 642 886 990
Size: 22 620 244 104
Fragments arranged by size:
620 244 104 22
BbeI G_GCGC'C
Cuts at: 0 654 990
Size: 654 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 294 328 990
Size: 294 34 662
Fragments arranged by size:
662 294 34
BclI T'GATC_A
Cuts at: 0 431 990
Size: 431 559
BglI GCCn_nnn'nGGC
Cuts at: 0 88 537 990
Size: 88 449 453
Fragments arranged by size:
453 449 88
BlpI GC'TnA_GC
Cuts at: 0 145 990
Size: 145 845
Bme1580I G_kGCm'C
Cuts at: 0 134 642 990
Size: 134 508 348
Fragments arranged by size:
508 348 134
BmgBI CAC'GTC
Cuts at: 0 522 990
Size: 522 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 990
Size: 562 428
BsaAI yAC'GTr
Cuts at: 0 290 990
Size: 290 700
BsaHI Gr'CG_yC
Cuts at: 0 651 990
Size: 651 339
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 990
Size: 340 30 620
Fragments arranged by size:
620 340 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 876 990
Size: 157 719 114
Fragments arranged by size:
719 157 114
BseYI C'CCAG_C
Cuts at: 0 116 623 990
Size: 116 507 367
Fragments arranged by size:
507 367 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 364 405 990
Size: 107 257 41 585
Fragments arranged by size:
585 257 107 41
BsiHKAI G_wGCw'C
Cuts at: 0 22 134 560 990
Size: 22 112 426 430
Fragments arranged by size:
430 426 112 22
Bsp1286I G_dGCh'C
Cuts at: 0 22 134 560 642 886 990
Size: 22 112 426 82 244 104
Fragments arranged by size:
426 244 112 104 82 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 453 496 855 990
Size: 236 217 43 359 135
Fragments arranged by size:
359 236 217 135 43
BsrDI GCAATG_nn'
Cuts at: 0 483 990
Size: 483 507
BsrFI r'CCGG_y
Cuts at: 0 362 990
Size: 362 628
BssSI C'ACGA_G
Cuts at: 0 252 990
Size: 252 738
BstAPI GCAn_nnn'nTGC
Cuts at: 0 281 870 990
Size: 281 589 120
Fragments arranged by size:
589 281 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 78 990
Size: 78 912
BstYI r'GATC_y
Cuts at: 0 104 990
Size: 104 886
BtgI C'CryG_G
Cuts at: 0 572 659 773 990
Size: 572 87 114 217
Fragments arranged by size:
572 217 114 87
BtsI GCAGTG_nn'
Cuts at: 0 417 990
Size: 417 573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 755 790 990
Size: 755 35 200
Fragments arranged by size:
755 200 35
DraIII CAC_nnn'GTG
Cuts at: 0 729 990
Size: 729 261
EaeI y'GGCC_r
Cuts at: 0 158 990
Size: 158 832
EarI CTCTTCn'nnn_
Cuts at: 0 10 565 820 831 990
Size: 10 555 255 11 159
Fragments arranged by size:
555 255 159 11 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 97 117 990
Size: 97 20 873
Fragments arranged by size:
873 97 20
EcoICRI GAG'CTC
Cuts at: 0 20 990
Size: 20 970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 815 990
Size: 562 253 175
Fragments arranged by size:
562 253 175
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 990
Size: 232 758
EcoO109I rG'GnC_Cy
Cuts at: 0 638 990
Size: 638 352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 39 990
Size: 39 951
FspI TGC'GCA
Cuts at: 0 841 990
Size: 841 149
HaeII r_GCGC'y
Cuts at: 0 321 654 990
Size: 321 333 336
Fragments arranged by size:
336 333 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 372 404 990
Size: 193 32 147 32 586
Fragments arranged by size:
586 193 147 32 32
KasI G'GCGC_C
Cuts at: 0 650 990
Size: 650 340
KpnI G_GTAC'C
Cuts at: 0 228 990
Size: 228 762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 990
Size: 497 493
MslI CAynn'nnrTG
Cuts at: 0 427 571 990
Size: 427 144 419
Fragments arranged by size:
427 419 144
MspA1I CmG'CkG
Cuts at: 0 221 990
Size: 221 769
NaeI GCC'GGC
Cuts at: 0 364 990
Size: 364 626
NarI GG'CG_CC
Cuts at: 0 651 990
Size: 651 339
NcoI C'CATG_G
Cuts at: 0 572 990
Size: 572 418
NgoMIV G'CCGG_C
Cuts at: 0 362 990
Size: 362 628
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 990
Size: 729 261
PmlI CAC'GTG
Cuts at: 0 290 990
Size: 290 700
PspOMI G'GGCC_C
Cuts at: 0 638 990
Size: 638 352
PstI C_TGCA'G
Cuts at: 0 286 866 990
Size: 286 580 124
Fragments arranged by size:
580 286 124
SacI G_AGCT'C
Cuts at: 0 22 990
Size: 22 968
SapI GCTCTTCn'nnn_
Cuts at: 0 10 565 820 990
Size: 10 555 255 170
Fragments arranged by size:
555 255 170 10
SexAI A'CCwGG_T
Cuts at: 0 725 990
Size: 725 265
SfcI C'TryA_G
Cuts at: 0 267 282 862 983 990
Size: 267 15 580 121 7
Fragments arranged by size:
580 267 121 15 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 88 990
Size: 88 902
SfoI GGC'GCC
Cuts at: 0 652 990
Size: 652 338
StyI C'CwwG_G
Cuts at: 0 233 572 990
Size: 233 339 418
Fragments arranged by size:
418 339 233
TatI w'GTAC_w
Cuts at: 0 347 990
Size: 347 643
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AloI AlwNI
ApaI ApaLI BamHI BanI BanII BbeI BcgI BclI
BglI BlpI Bme1580I BmgBI BpmI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I KasI KpnI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV PflMI PmlI PspOMI PstI
SacI SapI SexAI SfcI SfiI SfoI StyI TatI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AlfI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbsI BbvCI
BciVI BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DrdI EagI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI MfeI MluI MscI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI