Restriction map for
pF1KB9824
(Linear) (Six-base) MAPSORT of: KIBB9824.seq Check: 5993 from: 1 to: 945
KIBB9824.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AflII C'TTAA_G
Cuts at: 0 487 945
Size: 487 458
AflIII A'CryG_T
Cuts at: 0 114 945
Size: 114 831
AlwNI CAG_nnn'CTG
Cuts at: 0 402 945
Size: 402 543
ApaLI G'TGCA_C
Cuts at: 0 564 945
Size: 564 381
AvrII C'CTAG_G
Cuts at: 0 274 922 945
Size: 274 648 23
Fragments arranged by size:
648 274 23
BanII G_rGCy'C
Cuts at: 0 15 83 200 315 473 945
Size: 15 68 117 115 158 472
Fragments arranged by size:
472 158 117 115 68 15
BglI GCCn_nnn'nGGC
Cuts at: 0 276 945
Size: 276 669
Bme1580I G_kGCm'C
Cuts at: 0 568 945
Size: 568 377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 115 945
Size: 115 830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 945
Size: 265 680
Bpu10I CC'TnA_GC
Cuts at: 0 257 345 945
Size: 257 88 600
Fragments arranged by size:
600 257 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 457 945
Size: 184 273 488
Fragments arranged by size:
488 273 184
BsaI GGTCTCn'nnnn_
Cuts at: 0 676 945
Size: 676 269
BsaHI Gr'CG_yC
Cuts at: 0 49 95 945
Size: 49 46 850
Fragments arranged by size:
850 49 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 200 378 411 945
Size: 200 178 33 534
Fragments arranged by size:
534 200 178 33
BseYI C'CCAG_C
Cuts at: 0 104 945
Size: 104 841
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 502 945
Size: 290 212 443
Fragments arranged by size:
443 290 212
BsiHKAI G_wGCw'C
Cuts at: 0 15 263 473 568 945
Size: 15 248 210 95 377
Fragments arranged by size:
377 248 210 95 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 86 945
Size: 86 859
Bsp1286I G_dGCh'C
Cuts at: 0 15 83 200 263 315 473 568 945
Size: 15 68 117 63 52 158 95 377
Fragments arranged by size:
377 158 117 95 68 63 52 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 282 945
Size: 99 183 663
Fragments arranged by size:
663 183 99
BsrGI T'GTAC_A
Cuts at: 0 748 945
Size: 748 197
BssSI C'ACGA_G
Cuts at: 0 235 308 945
Size: 235 73 637
Fragments arranged by size:
637 235 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 39 945
Size: 39 906
BstXI CCAn_nnnn'nTGG
Cuts at: 0 593 945
Size: 593 352
BstYI r'GATC_y
Cuts at: 0 325 596 945
Size: 325 271 349
Fragments arranged by size:
349 325 271
BtgI C'CryG_G
Cuts at: 0 246 764 945
Size: 246 518 181
Fragments arranged by size:
518 246 181
BtsI GCAGTG_nn'
Cuts at: 0 616 945
Size: 616 329
DrdI GACnn_nn'nnGTC
Cuts at: 0 248 945
Size: 248 697
EarI CTCTTCn'nnn_
Cuts at: 0 679 945
Size: 679 266
EcoICRI GAG'CTC
Cuts at: 0 13 471 945
Size: 13 458 474
Fragments arranged by size:
474 458 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 945
Size: 265 680
EcoNI CCTnn'n_nnAGG
Cuts at: 0 573 945
Size: 573 372
EcoO109I rG'GnC_Cy
Cuts at: 0 172 945
Size: 172 773
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 182 214 945
Size: 182 32 731
Fragments arranged by size:
731 182 32
HincII GTy'rAC
Cuts at: 0 858 945
Size: 858 87
HpaI GTT'AAC
Cuts at: 0 858 945
Size: 858 87
MspA1I CmG'CkG
Cuts at: 0 875 945
Size: 875 70
NcoI C'CATG_G
Cuts at: 0 764 945
Size: 764 181
NspI r_CATG'y
Cuts at: 0 118 945
Size: 118 827
PciI A'CATG_T
Cuts at: 0 114 945
Size: 114 831
PflMI CCAn_nnn'nTGG
Cuts at: 0 193 945
Size: 193 752
PstI C_TGCA'G
Cuts at: 0 47 945
Size: 47 898
SacI G_AGCT'C
Cuts at: 0 15 473 945
Size: 15 458 472
Fragments arranged by size:
472 458 15
SfcI C'TryA_G
Cuts at: 0 43 525 945
Size: 43 482 420
Fragments arranged by size:
482 420 43
SmlI C'TyrA_G
Cuts at: 0 199 472 487 945
Size: 199 273 15 458
Fragments arranged by size:
458 273 199 15
StuI AGG'CCT
Cuts at: 0 579 864 945
Size: 579 285 81
Fragments arranged by size:
579 285 81
StyI C'CwwG_G
Cuts at: 0 274 574 764 815 922 945
Size: 274 300 190 51 107 23
Fragments arranged by size:
300 274 190 107 51 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 757 945
Size: 118 639 188
Fragments arranged by size:
639 188 118
TatI w'GTAC_w
Cuts at: 0 161 748 945
Size: 161 587 197
Fragments arranged by size:
587 197 161
Enzymes that do cut:
AflII AflIII AlwNI ApaLI AvrII BanII BglI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrGI BssSI BstAPI
BstXI BstYI BtgI BtsI DrdI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HincII HpaI MspA1I NcoI NspI
PciI PflMI PstI SacI SfcI SmlI StuI StyI
TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI