Restriction map for
pF1KB9823
(Linear) (Six-base) MAPSORT of: KIBB9823.seq Check: 5035 from: 1 to: 942
KIBB9823.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 547 942
Size: 547 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 942
Size: 435 507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 107 128 942
Size: 107 21 814
Fragments arranged by size:
814 107 21
ApaI G_GGCC'C
Cuts at: 0 831 942
Size: 831 111
ApoI r'AATT_y
Cuts at: 0 423 942
Size: 423 519
BanI G'GyrC_C
Cuts at: 0 156 942
Size: 156 786
BanII G_rGCy'C
Cuts at: 0 259 831 942
Size: 259 572 111
Fragments arranged by size:
572 259 111
BglI GCCn_nnn'nGGC
Cuts at: 0 569 942
Size: 569 373
Bme1580I G_kGCm'C
Cuts at: 0 590 648 831 942
Size: 590 58 183 111
Fragments arranged by size:
590 183 111 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 942
Size: 132 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 258 322 787 942
Size: 243 15 64 465 155
Fragments arranged by size:
465 243 155 64 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 67 349 942
Size: 28 39 282 593
Fragments arranged by size:
593 282 39 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 108 111 114 115 352 667 682
Size: 30 78 3 3 1 237 315 15
Cuts at: 682 942
Size: 260
Fragments arranged by size:
315 260 237 78 30 15 3 3
1
BseYI C'CCAG_C
Cuts at: 0 405 567 942
Size: 405 162 375
Fragments arranged by size:
405 375 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 844 942
Size: 772 72 98
Fragments arranged by size:
772 98 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 298 942
Size: 298 644
Bsp1286I G_dGCh'C
Cuts at: 0 259 590 648 831 942
Size: 259 331 58 183 111
Fragments arranged by size:
331 259 183 111 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 547 768 942
Size: 547 221 174
Fragments arranged by size:
547 221 174
BsrFI r'CCGG_y
Cuts at: 0 773 942
Size: 773 169
BsrGI T'GTAC_A
Cuts at: 0 524 942
Size: 524 418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 867 942
Size: 867 75
Bsu36I CC'TnA_GG
Cuts at: 0 35 194 650 942
Size: 35 159 456 292
Fragments arranged by size:
456 292 159 35
BtsI GCAGTG_nn'
Cuts at: 0 24 795 942
Size: 24 771 147
Fragments arranged by size:
771 147 24
EaeI y'GGCC_r
Cuts at: 0 516 617 942
Size: 516 101 325
Fragments arranged by size:
516 325 101
EarI CTCTTCn'nnn_
Cuts at: 0 932 942
Size: 932 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 258 322 435 787 942
Size: 243 15 64 113 352 155
Fragments arranged by size:
352 243 155 113 64 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 38 151 942
Size: 38 113 791
Fragments arranged by size:
791 113 38
EcoO109I rG'GnC_Cy
Cuts at: 0 63 780 827 828 886 942
Size: 63 717 47 1 58 56
Fragments arranged by size:
717 63 58 56 47 1
FspI TGC'GCA
Cuts at: 0 83 719 942
Size: 83 636 223
Fragments arranged by size:
636 223 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 942
Size: 241 32 669
Fragments arranged by size:
669 241 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 942
Size: 662 280
MscI TGG'CCA
Cuts at: 0 619 942
Size: 619 323
MslI CAynn'nnrTG
Cuts at: 0 493 942
Size: 493 449
MspA1I CmG'CkG
Cuts at: 0 477 942
Size: 477 465
NspI r_CATG'y
Cuts at: 0 803 942
Size: 803 139
PasI CC'CwG_GG
Cuts at: 0 66 942
Size: 66 876
PflMI CCAn_nnn'nTGG
Cuts at: 0 747 942
Size: 747 195
PfoI T'CCnGG_A
Cuts at: 0 428 942
Size: 428 514
PpuMI rG'GwC_Cy
Cuts at: 0 780 886 942
Size: 780 106 56
Fragments arranged by size:
780 106 56
PspOMI G'GGCC_C
Cuts at: 0 827 942
Size: 827 115
PvuII CAG'CTG
Cuts at: 0 477 942
Size: 477 465
ScaI AGT'ACT
Cuts at: 0 469 942
Size: 469 473
SmlI C'TyrA_G
Cuts at: 0 7 46 364 942
Size: 7 39 318 578
Fragments arranged by size:
578 318 39 7
SphI G_CATG'C
Cuts at: 0 803 942
Size: 803 139
StuI AGG'CCT
Cuts at: 0 44 599 942
Size: 44 555 343
Fragments arranged by size:
555 343 44
StyI C'CwwG_G
Cuts at: 0 54 823 942
Size: 54 769 119
Fragments arranged by size:
769 119 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 754 942
Size: 67 687 188
Fragments arranged by size:
687 188 67
TatI w'GTAC_w
Cuts at: 0 467 524 942
Size: 467 57 418
Fragments arranged by size:
467 418 57
Tth111I GACn'n_nGTC
Cuts at: 0 709 942
Size: 709 233
Enzymes that do cut:
AarI AcuI AlfI ApaI ApoI BanI BanII BglI
Bme1580I BplI BpmI BpuEI BseRI BseYI BsgI BsmBI
Bsp1286I BspMI BsrFI BsrGI BstAPI Bsu36I BtsI EaeI
EarI Eco57MI EcoNI EcoO109I FspI Hin4I MmeI MscI
MslI MspA1I NspI PasI PflMI PfoI PpuMI PspOMI
PvuII ScaI SmlI SphI StuI StyI TaqII TatI
Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TstI XbaI XcmI XhoI
XmaI XmnI ZraI