Restriction map for
pF1KB9821
(Linear) (Six-base) MAPSORT of: KIBB9821.seq Check: 5656 from: 1 to: 936
KIBB9821.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 785 936
Size: 785 151
AvaI C'yCGr_G
Cuts at: 0 453 936
Size: 453 483
BanI G'GyrC_C
Cuts at: 0 915 936
Size: 915 21
BanII G_rGCy'C
Cuts at: 0 202 250 301 748 808 936
Size: 202 48 51 447 60 128
Fragments arranged by size:
447 202 128 60 51 48
BbeI G_GCGC'C
Cuts at: 0 919 936
Size: 919 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 58 936
Size: 58 878
BbvCI CC'TCA_GC
Cuts at: 0 29 936
Size: 29 907
BglI GCCn_nnn'nGGC
Cuts at: 0 924 936
Size: 924 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 31 936
Size: 31 905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 372 516 708 851 936
Size: 297 75 144 192 143 85
Fragments arranged by size:
297 192 144 143 85 75
Bpu10I CC'TnA_GC
Cuts at: 0 29 123 936
Size: 29 94 813
Fragments arranged by size:
813 94 29
BsaHI Gr'CG_yC
Cuts at: 0 488 881 916 936
Size: 488 393 35 20
Fragments arranged by size:
488 393 35 20
BsaWI w'CCGG_w
Cuts at: 0 250 271 346 936
Size: 250 21 75 590
Fragments arranged by size:
590 250 75 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 192 276 333 351 396 423 495 588
Size: 192 84 57 18 45 27 72 93
Cuts at: 588 618 756 840 936
Size: 30 138 84 96
Fragments arranged by size:
192 138 96 93 84 84 72 57
45 30 27 18
BseYI C'CCAG_C
Cuts at: 0 662 902 936
Size: 662 240 34
Fragments arranged by size:
662 240 34
BsiHKAI G_wGCw'C
Cuts at: 0 748 936
Size: 748 188
BsmBI CGTCTCn'nnnn_
Cuts at: 0 495 630 936
Size: 495 135 306
Fragments arranged by size:
495 306 135
Bsp1286I G_dGCh'C
Cuts at: 0 202 250 301 748 808 936
Size: 202 48 51 447 60 128
Fragments arranged by size:
447 202 128 60 51 48
BspEI T'CCGG_A
Cuts at: 0 271 936
Size: 271 665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 627 936
Size: 627 309
BssHII G'CGCG_C
Cuts at: 0 656 936
Size: 656 280
BssSI C'ACGA_G
Cuts at: 0 651 936
Size: 651 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 785 936
Size: 785 151
BtgI C'CryG_G
Cuts at: 0 854 936
Size: 854 82
EaeI y'GGCC_r
Cuts at: 0 925 936
Size: 925 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 605 722 936
Size: 605 117 214
Fragments arranged by size:
605 214 117
EcoICRI GAG'CTC
Cuts at: 0 746 936
Size: 746 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 372 516 708 851 936
Size: 297 75 144 192 143 85
Fragments arranged by size:
297 192 144 143 85 75
HaeII r_GCGC'y
Cuts at: 0 919 936
Size: 919 17
KasI G'GCGC_C
Cuts at: 0 915 936
Size: 915 21
MspA1I CmG'CkG
Cuts at: 0 86 140 317 392 536 611 704 728
Size: 86 54 177 75 144 75 93 24
Cuts at: 728 782 936
Size: 54 154
Fragments arranged by size:
177 154 144 93 86 75 75 54
54 24
NarI GG'CG_CC
Cuts at: 0 916 936
Size: 916 20
PfoI T'CCnGG_A
Cuts at: 0 769 865 936
Size: 769 96 71
Fragments arranged by size:
769 96 71
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 126 158 936
Size: 126 32 778
Fragments arranged by size:
778 126 32
SacI G_AGCT'C
Cuts at: 0 748 936
Size: 748 188
ScaI AGT'ACT
Cuts at: 0 268 936
Size: 268 668
SfoI GGC'GCC
Cuts at: 0 917 936
Size: 917 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 847 936
Size: 847 89
TatI w'GTAC_w
Cuts at: 0 266 936
Size: 266 670
Enzymes that do cut:
AlwNI AvaI BanI BanII BbeI BbsI BbvCI BglI
BmrI BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BssHII BssSI BstAPI BtgI
EaeI EciI EcoICRI Eco57MI HaeII KasI MspA1I NarI
PfoI PsrI SacI ScaI SfoI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BspHI BsrBI BsrDI BsrFI BsrGI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI