Restriction map for
pF1KB9820
(Linear) (Six-base) MAPSORT of: KIBB9820.seq Check: 6991 from: 1 to: 933
KIBB9820.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AflII C'TTAA_G
Cuts at: 0 769 933
Size: 769 164
AhdI GACnn_n'nnGTC
Cuts at: 0 354 933
Size: 354 579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 152 933
Size: 152 781
ApoI r'AATT_y
Cuts at: 0 25 933
Size: 25 908
BanI G'GyrC_C
Cuts at: 0 756 827 933
Size: 756 71 106
Fragments arranged by size:
756 106 71
BanII G_rGCy'C
Cuts at: 0 461 933
Size: 461 472
BbvCI CC'TCA_GC
Cuts at: 0 332 476 933
Size: 332 144 457
Fragments arranged by size:
457 332 144
BciVI GTATCCnnnnn_n'
Cuts at: 0 445 933
Size: 445 488
BglI GCCn_nnn'nGGC
Cuts at: 0 269 933
Size: 269 664
BglII A'GATC_T
Cuts at: 0 650 933
Size: 650 283
BlpI GC'TnA_GC
Cuts at: 0 125 933
Size: 125 808
Bme1580I G_kGCm'C
Cuts at: 0 265 830 933
Size: 265 565 103
Fragments arranged by size:
565 265 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 78 188 287 440 933
Size: 78 110 99 153 493
Fragments arranged by size:
493 153 110 99 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 484 933
Size: 387 97 449
Fragments arranged by size:
449 387 97
Bpu10I CC'TnA_GC
Cuts at: 0 332 476 858 933
Size: 332 144 382 75
Fragments arranged by size:
382 332 144 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 183 933
Size: 183 750
BsaWI w'CCGG_w
Cuts at: 0 696 933
Size: 696 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 180 210 478 508 933
Size: 180 30 268 30 425
Fragments arranged by size:
425 268 180 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 201 484 859 933
Size: 201 283 375 74
Fragments arranged by size:
375 283 201 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 933
Size: 664 269
BsiHKAI G_wGCw'C
Cuts at: 0 118 461 715 933
Size: 118 343 254 218
Fragments arranged by size:
343 254 218 118
Bsp1286I G_dGCh'C
Cuts at: 0 118 265 461 715 830 933
Size: 118 147 196 254 115 103
Fragments arranged by size:
254 196 147 118 115 103
BspEI T'CCGG_A
Cuts at: 0 696 933
Size: 696 237
BssSI C'ACGA_G
Cuts at: 0 851 933
Size: 851 82
BstYI r'GATC_y
Cuts at: 0 306 650 933
Size: 306 344 283
Fragments arranged by size:
344 306 283
BtgI C'CryG_G
Cuts at: 0 42 933
Size: 42 891
EaeI y'GGCC_r
Cuts at: 0 45 933
Size: 45 888
EarI CTCTTCn'nnn_
Cuts at: 0 178 461 703 933
Size: 178 283 242 230
Fragments arranged by size:
283 242 230 178
EcoICRI GAG'CTC
Cuts at: 0 459 933
Size: 459 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 484 933
Size: 387 97 449
Fragments arranged by size:
449 387 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 570 933
Size: 570 363
EcoO109I rG'GnC_Cy
Cuts at: 0 51 282 575 933
Size: 51 231 293 358
Fragments arranged by size:
358 293 231 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 180 212 340 372 478 510
Size: 100 32 48 32 128 32 106 32
Cuts at: 510 933
Size: 423
Fragments arranged by size:
423 128 106 100 48 32 32 32
32
HincII GTy'rAC
Cuts at: 0 914 933
Size: 914 19
MfeI C'AATT_G
Cuts at: 0 585 933
Size: 585 348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 933
Size: 132 801
MscI TGG'CCA
Cuts at: 0 47 933
Size: 47 886
NcoI C'CATG_G
Cuts at: 0 42 933
Size: 42 891
PasI CC'CwG_GG
Cuts at: 0 55 933
Size: 55 878
PflMI CCAn_nnn'nTGG
Cuts at: 0 233 933
Size: 233 700
PfoI T'CCnGG_A
Cuts at: 0 737 933
Size: 737 196
PpuMI rG'GwC_Cy
Cuts at: 0 51 933
Size: 51 882
SacI G_AGCT'C
Cuts at: 0 461 933
Size: 461 472
SapI GCTCTTCn'nnn_
Cuts at: 0 703 933
Size: 703 230
SmlI C'TyrA_G
Cuts at: 0 769 933
Size: 769 164
StyI C'CwwG_G
Cuts at: 0 42 267 933
Size: 42 225 666
Fragments arranged by size:
666 225 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 933
Size: 231 702
Tth111I GACn'n_nGTC
Cuts at: 0 194 933
Size: 194 739
Enzymes that do cut:
AflII AhdI AlfI ApoI BanI BanII BbvCI BciVI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI
BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI BssSI
BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HincII MfeI MmeI MscI NcoI PasI PflMI
PfoI PpuMI SacI SapI SmlI StyI TaqII Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BclI BfrBI BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MluI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XhoI XmaI XmnI ZraI