Restriction map for
pF1KB9819
(Linear) (Six-base) MAPSORT of: KIBB9819.seq Check: 5810 from: 1 to: 930
KIBB9819.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 440 824 930
Size: 440 384 106
Fragments arranged by size:
440 384 106
AleI CACnn'nnGTG
Cuts at: 0 566 930
Size: 566 364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 584 930
Size: 584 346
ApaI G_GGCC'C
Cuts at: 0 89 930
Size: 89 841
ApaLI G'TGCA_C
Cuts at: 0 427 930
Size: 427 503
AvaI C'yCGr_G
Cuts at: 0 325 724 874 930
Size: 325 399 150 56
Fragments arranged by size:
399 325 150 56
BanI G'GyrC_C
Cuts at: 0 568 620 878 930
Size: 568 52 258 52
Fragments arranged by size:
568 258 52 52
BanII G_rGCy'C
Cuts at: 0 89 301 806 930
Size: 89 212 505 124
Fragments arranged by size:
505 212 124 89
BbeI G_GCGC'C
Cuts at: 0 882 930
Size: 882 48
BclI T'GATC_A
Cuts at: 0 749 930
Size: 749 181
BfrBI ATG'CAT
Cuts at: 0 272 930
Size: 272 658
BglI GCCn_nnn'nGGC
Cuts at: 0 156 488 629 684 876 930
Size: 156 332 141 55 192 54
Fragments arranged by size:
332 192 156 141 55 54
BlpI GC'TnA_GC
Cuts at: 0 526 586 930
Size: 526 60 344
Fragments arranged by size:
526 344 60
Bme1580I G_kGCm'C
Cuts at: 0 89 431 930
Size: 89 342 499
Fragments arranged by size:
499 342 89
BmgBI CAC'GTC
Cuts at: 0 132 930
Size: 132 798
BmrI ACTGGGnnnn_n'
Cuts at: 0 405 930
Size: 405 525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 516 930
Size: 463 53 414
Fragments arranged by size:
463 414 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 105 930
Size: 105 825
BsaAI yAC'GTr
Cuts at: 0 564 930
Size: 564 366
BsaHI Gr'CG_yC
Cuts at: 0 470 879 930
Size: 470 409 51
Fragments arranged by size:
470 409 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 358 388 855 885 905 930
Size: 97 30 231 30 467 30 20 25
Fragments arranged by size:
467 231 97 30 30 30 25 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 850 916 919 930
Size: 850 66 3 11
Fragments arranged by size:
850 66 11 3
BseYI C'CCAG_C
Cuts at: 0 206 892 930
Size: 206 686 38
Fragments arranged by size:
686 206 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 418 802 930
Size: 217 201 384 128
Fragments arranged by size:
384 217 201 128
BsiEI CG_ry'CG
Cuts at: 0 56 492 791 930
Size: 56 436 299 139
Fragments arranged by size:
436 299 139 56
BsiHKAI G_wGCw'C
Cuts at: 0 431 806 930
Size: 431 375 124
Fragments arranged by size:
431 375 124
BsmI GAATG_Cn'
Cuts at: 0 250 274 930
Size: 250 24 656
Fragments arranged by size:
656 250 24
Bsp1286I G_dGCh'C
Cuts at: 0 89 301 431 806 930
Size: 89 212 130 375 124
Fragments arranged by size:
375 212 130 124 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 440 824 930
Size: 341 99 384 106
Fragments arranged by size:
384 341 106 99
BsrFI r'CCGG_y
Cuts at: 0 437 683 930
Size: 437 246 247
Fragments arranged by size:
437 247 246
BssHII G'CGCG_C
Cuts at: 0 783 930
Size: 783 147
BssSI C'ACGA_G
Cuts at: 0 235 930
Size: 235 695
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 930
Size: 540 390
Bsu36I CC'TnA_GG
Cuts at: 0 152 930
Size: 152 778
BtgI C'CryG_G
Cuts at: 0 50 226 539 930
Size: 50 176 313 391
Fragments arranged by size:
391 313 176 50
BtsI GCAGTG_nn'
Cuts at: 0 926 930
Size: 926 4
DraIII CAC_nnn'GTG
Cuts at: 0 240 930
Size: 240 690
EaeI y'GGCC_r
Cuts at: 0 53 489 788 930
Size: 53 436 299 142
Fragments arranged by size:
436 299 142 53
EagI C'GGCC_G
Cuts at: 0 53 489 788 930
Size: 53 436 299 142
Fragments arranged by size:
436 299 142 53
EarI CTCTTCn'nnn_
Cuts at: 0 277 930
Size: 277 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 841 930
Size: 841 89
EcoICRI GAG'CTC
Cuts at: 0 804 930
Size: 804 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 516 930
Size: 463 53 414
Fragments arranged by size:
463 414 53
EcoO109I rG'GnC_Cy
Cuts at: 0 86 744 930
Size: 86 658 186
Fragments arranged by size:
658 186 86
FspI TGC'GCA
Cuts at: 0 547 664 930
Size: 547 117 266
Fragments arranged by size:
547 266 117
HaeII r_GCGC'y
Cuts at: 0 310 539 882 930
Size: 310 229 343 48
Fragments arranged by size:
343 310 229 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 930
Size: 358 32 540
Fragments arranged by size:
540 358 32
HincII GTy'rAC
Cuts at: 0 501 930
Size: 501 429
KasI G'GCGC_C
Cuts at: 0 878 930
Size: 878 52
MslI CAynn'nnrTG
Cuts at: 0 566 930
Size: 566 364
MspA1I CmG'CkG
Cuts at: 0 228 699 930
Size: 228 471 231
Fragments arranged by size:
471 231 228
NarI GG'CG_CC
Cuts at: 0 879 930
Size: 879 51
NotI GC'GGCC_GC
Cuts at: 0 489 930
Size: 489 441
NsiI A_TGCA'T
Cuts at: 0 274 930
Size: 274 656
PmlI CAC'GTG
Cuts at: 0 564 930
Size: 564 366
PpuMI rG'GwC_Cy
Cuts at: 0 744 930
Size: 744 186
PspOMI G'GGCC_C
Cuts at: 0 85 930
Size: 85 845
PspXI vC'TCGA_Gb
Cuts at: 0 724 930
Size: 724 206
PstI C_TGCA'G
Cuts at: 0 692 800 930
Size: 692 108 130
Fragments arranged by size:
692 130 108
PvuII CAG'CTG
Cuts at: 0 699 930
Size: 699 231
SacI G_AGCT'C
Cuts at: 0 806 930
Size: 806 124
SacII CC_GC'GG
Cuts at: 0 229 930
Size: 229 701
SapI GCTCTTCn'nnn_
Cuts at: 0 277 930
Size: 277 653
SexAI A'CCwGG_T
Cuts at: 0 8 930
Size: 8 922
SfcI C'TryA_G
Cuts at: 0 688 796 930
Size: 688 108 134
Fragments arranged by size:
688 134 108
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 488 930
Size: 488 442
SfoI GGC'GCC
Cuts at: 0 880 930
Size: 880 50
SmaI CCC'GGG
Cuts at: 0 876 930
Size: 876 54
SmlI C'TyrA_G
Cuts at: 0 724 930
Size: 724 206
StyI C'CwwG_G
Cuts at: 0 572 930
Size: 572 358
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 930
Size: 556 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 905 930
Size: 905 25
XbaI T'CTAG_A
Cuts at: 0 738 930
Size: 738 192
XhoI C'TCGA_G
Cuts at: 0 724 930
Size: 724 206
XmaI C'CCGG_G
Cuts at: 0 874 930
Size: 874 56
XmnI GAAnn'nnTTC
Cuts at: 0 286 930
Size: 286 644
Enzymes that do cut:
AarI AleI AlfI ApaI ApaLI AvaI BanI BanII
BbeI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssHII BssSI
BstXI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII
KasI MslI MspA1I NarI NotI NsiI PmlI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SapI SexAI
SfcI SfiI SfoI SmaI SmlI StyI TaqII TstI
XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BglII
BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII
BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NruI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI
SanDI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XcmI ZraI