Restriction map for
pF1KB9814
(Linear) (Six-base) MAPSORT of: KIBB9814.seq Check: 6851 from: 1 to: 882
KIBB9814.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 882
Size: 416 466
AhdI GACnn_n'nnGTC
Cuts at: 0 409 882
Size: 409 473
ApaI G_GGCC'C
Cuts at: 0 514 882
Size: 514 368
ApoI r'AATT_y
Cuts at: 0 111 882
Size: 111 771
AvaI C'yCGr_G
Cuts at: 0 180 867 882
Size: 180 687 15
Fragments arranged by size:
687 180 15
BanI G'GyrC_C
Cuts at: 0 370 882
Size: 370 512
BanII G_rGCy'C
Cuts at: 0 233 429 492 514 695 794 882
Size: 233 196 63 22 181 99 88
Fragments arranged by size:
233 196 181 99 88 63 22
BglI GCCn_nnn'nGGC
Cuts at: 0 41 583 882
Size: 41 542 299
Fragments arranged by size:
542 299 41
BglII A'GATC_T
Cuts at: 0 530 882
Size: 530 352
Bme1580I G_kGCm'C
Cuts at: 0 138 514 882
Size: 138 376 368
Fragments arranged by size:
376 368 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 135 634 828 882
Size: 135 499 194 54
Fragments arranged by size:
499 194 135 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 344 580 882
Size: 337 7 236 302
Fragments arranged by size:
337 302 236 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 284 882
Size: 256 28 598
Fragments arranged by size:
598 256 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 372 757 857 882
Size: 372 385 100 25
Fragments arranged by size:
385 372 100 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 241 865 882
Size: 93 148 624 17
Fragments arranged by size:
624 148 93 17
BseYI C'CCAG_C
Cuts at: 0 642 882
Size: 642 240
BsiHKAI G_wGCw'C
Cuts at: 0 492 595 882
Size: 492 103 287
Fragments arranged by size:
492 287 103
Bsp1286I G_dGCh'C
Cuts at: 0 138 233 429 492 514 595 695 794
Size: 138 95 196 63 22 81 100 99
Cuts at: 794 882
Size: 88
Fragments arranged by size:
196 138 100 99 95 88 81 63
22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 93 882
Size: 93 789
BstYI r'GATC_y
Cuts at: 0 530 882
Size: 530 352
BtgI C'CryG_G
Cuts at: 0 146 882
Size: 146 736
BtsI GCAGTG_nn'
Cuts at: 0 601 882
Size: 601 281
EaeI y'GGCC_r
Cuts at: 0 809 882
Size: 809 73
EarI CTCTTCn'nnn_
Cuts at: 0 512 882
Size: 512 370
EcoICRI GAG'CTC
Cuts at: 0 490 882
Size: 490 392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 344 416 580 882
Size: 337 7 72 164 302
Fragments arranged by size:
337 302 164 72 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 419 882
Size: 419 463
EcoO109I rG'GnC_Cy
Cuts at: 0 510 882
Size: 510 372
EcoRI G'AATT_C
Cuts at: 0 111 882
Size: 111 771
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 395 427 537 569 882
Size: 88 32 275 32 110 32 313
Fragments arranged by size:
313 275 110 88 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 882
Size: 815 67
MspA1I CmG'CkG
Cuts at: 0 642 882
Size: 642 240
NcoI C'CATG_G
Cuts at: 0 146 882
Size: 146 736
PasI CC'CwG_GG
Cuts at: 0 415 882
Size: 415 467
PflMI CCAn_nnn'nTGG
Cuts at: 0 818 882
Size: 818 64
PfoI T'CCnGG_A
Cuts at: 0 785 882
Size: 785 97
PspOMI G'GGCC_C
Cuts at: 0 510 882
Size: 510 372
PstI C_TGCA'G
Cuts at: 0 530 761 882
Size: 530 231 121
Fragments arranged by size:
530 231 121
SacI G_AGCT'C
Cuts at: 0 492 882
Size: 492 390
SbfI CC_TGCA'GG
Cuts at: 0 761 882
Size: 761 121
SexAI A'CCwGG_T
Cuts at: 0 441 882
Size: 441 441
SfcI C'TryA_G
Cuts at: 0 526 757 882
Size: 526 231 125
Fragments arranged by size:
526 231 125
SmaI CCC'GGG
Cuts at: 0 869 882
Size: 869 13
SmlI C'TyrA_G
Cuts at: 0 271 299 882
Size: 271 28 583
Fragments arranged by size:
583 271 28
StuI AGG'CCT
Cuts at: 0 63 763 882
Size: 63 700 119
Fragments arranged by size:
700 119 63
StyI C'CwwG_G
Cuts at: 0 37 146 614 764 882
Size: 37 109 468 150 118
Fragments arranged by size:
468 150 118 109 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 882
Size: 153 729
XmaI C'CCGG_G
Cuts at: 0 867 882
Size: 867 15
XmnI GAAnn'nnTTC
Cuts at: 0 264 882
Size: 264 618
Enzymes that do cut:
AcuI AhdI ApaI ApoI AvaI BanI BanII BglI
BglII Bme1580I BmrI BpmI BpuEI BsaI BseRI BseYI
BsiHKAI Bsp1286I BstXI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I MmeI MspA1I
NcoI PasI PflMI PfoI PspOMI PstI SacI SbfI
SexAI SfcI SmaI SmlI StuI StyI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI ZraI