Restriction map for
pF1KB9809
(Linear) (Six-base) MAPSORT of: KIBB9809.seq Check: 2014 from: 1 to: 723
KIBB9809.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 410 723
Size: 410 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 624 723
Size: 218 406 99
Fragments arranged by size:
406 218 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 667 723
Size: 667 56
ApaI G_GGCC'C
Cuts at: 0 443 723
Size: 443 280
BanII G_rGCy'C
Cuts at: 0 443 723
Size: 443 280
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 271 593 723
Size: 70 201 322 130
Fragments arranged by size:
322 201 130 70
BglI GCCn_nnn'nGGC
Cuts at: 0 477 723
Size: 477 246
Bme1580I G_kGCm'C
Cuts at: 0 443 723
Size: 443 280
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 723
Size: 437 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 605 712 723
Size: 590 15 107 11
Fragments arranged by size:
590 107 15 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 22 723
Size: 22 701
BsaHI Gr'CG_yC
Cuts at: 0 418 723
Size: 418 305
BsaWI w'CCGG_w
Cuts at: 0 522 550 723
Size: 522 28 173
Fragments arranged by size:
522 173 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 723
Size: 308 30 385
Fragments arranged by size:
385 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 640 723
Size: 640 83
BseYI C'CCAG_C
Cuts at: 0 75 560 723
Size: 75 485 163
Fragments arranged by size:
485 163 75
BsiHKAI G_wGCw'C
Cuts at: 0 146 412 632 723
Size: 146 266 220 91
Fragments arranged by size:
266 220 146 91
BsmI GAATG_Cn'
Cuts at: 0 320 723
Size: 320 403
Bsp1286I G_dGCh'C
Cuts at: 0 146 412 443 632 723
Size: 146 266 31 189 91
Fragments arranged by size:
266 189 146 91 31
BspEI T'CCGG_A
Cuts at: 0 522 723
Size: 522 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 723
Size: 410 313
BsrFI r'CCGG_y
Cuts at: 0 426 723
Size: 426 297
BsrGI T'GTAC_A
Cuts at: 0 459 723
Size: 459 264
BstYI r'GATC_y
Cuts at: 0 188 519 723
Size: 188 331 204
Fragments arranged by size:
331 204 188
DrdI GACnn_nn'nnGTC
Cuts at: 0 273 723
Size: 273 450
EaeI y'GGCC_r
Cuts at: 0 366 428 723
Size: 366 62 295
Fragments arranged by size:
366 295 62
EarI CTCTTCn'nnn_
Cuts at: 0 236 723
Size: 236 487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 590 605 624 712 723
Size: 218 372 15 19 88 11
Fragments arranged by size:
372 218 88 19 15 11
EcoO109I rG'GnC_Cy
Cuts at: 0 439 723
Size: 439 284
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 723
Size: 183 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 308 340 511 543 723
Size: 308 32 171 32 180
Fragments arranged by size:
308 180 171 32 32
MscI TGG'CCA
Cuts at: 0 368 723
Size: 368 355
MspA1I CmG'CkG
Cuts at: 0 10 723
Size: 10 713
NaeI GCC'GGC
Cuts at: 0 428 723
Size: 428 295
NgoMIV G'CCGG_C
Cuts at: 0 426 723
Size: 426 297
PfoI T'CCnGG_A
Cuts at: 0 326 723
Size: 326 397
PspOMI G'GGCC_C
Cuts at: 0 439 723
Size: 439 284
PstI C_TGCA'G
Cuts at: 0 407 723
Size: 407 316
SfcI C'TryA_G
Cuts at: 0 403 723
Size: 403 320
StuI AGG'CCT
Cuts at: 0 226 723
Size: 226 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 412 723
Size: 412 311
TatI w'GTAC_w
Cuts at: 0 459 672 723
Size: 459 213 51
Fragments arranged by size:
459 213 51
Enzymes that do cut:
AarI AcuI AlfI ApaI BanII BbsI BglI Bme1580I
BmrI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI BsrGI BstYI
DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I MscI
MspA1I NaeI NgoMIV PfoI PspOMI PstI SfcI StuI
TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI