(Linear) (Six-base) MAPSORT of: KIBB9807.seq  Check: 2989  from: 1  to: 681



KIBB9807.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

ApaI G_GGCC'C

Cuts at:      0     648     681 
   Size:        648      33
 
ApoI r'AATT_y

Cuts at:      0     480     508     588     625     681 
   Size:        480      28      80      37      56

  Fragments arranged by size: 

                 480      80      56      37      28
 
AvrII C'CTAG_G

Cuts at:      0     610     681 
   Size:        610      71
 
BanII G_rGCy'C

Cuts at:      0     648     681 
   Size:        648      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     116     671     681 
   Size:        116     555      10

  Fragments arranged by size: 

                 555     116      10
 
BbvCI CC'TCA_GC

Cuts at:      0     389     681 
   Size:        389     292
 
Bme1580I G_kGCm'C

Cuts at:      0     648     681 
   Size:        648      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     389     681 
   Size:        389     292
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     681 
   Size:        433     248
 
BseYI C'CCAG_C

Cuts at:      0     384     681 
   Size:        384     297
 
BsmI GAATG_Cn'

Cuts at:      0      35     681 
   Size:         35     646
 
Bsp1286I G_dGCh'C

Cuts at:      0     648     681 
   Size:        648      33
 
BsrDI GCAATG_nn'

Cuts at:      0     495     681 
   Size:        495     186
 
BssSI C'ACGA_G

Cuts at:      0      76     681 
   Size:         76     605
 
BstBI TT'CG_AA

Cuts at:      0      37     681 
   Size:         37     644
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     681 
   Size:         16     665
 
EcoO109I rG'GnC_Cy

Cuts at:      0     597     644     645     681 
   Size:        597      47       1      36

  Fragments arranged by size: 

                 597      47      36       1
 
EcoRI G'AATT_C

Cuts at:      0     625     681 
   Size:        625      56
 
HaeII r_GCGC'y

Cuts at:      0     384     681 
   Size:        384     297
 
MspA1I CmG'CkG

Cuts at:      0     388     681 
   Size:        388     293
 
PpuMI rG'GwC_Cy

Cuts at:      0     597     681 
   Size:        597      84
 
PspOMI G'GGCC_C

Cuts at:      0     644     681 
   Size:        644      37
 
PstI C_TGCA'G

Cuts at:      0     375     643     681 
   Size:        375     268      38

  Fragments arranged by size: 

                 375     268      38
 
PvuII CAG'CTG

Cuts at:      0     388     681 
   Size:        388     293
 
SfcI C'TryA_G

Cuts at:      0     371     639     681 
   Size:        371     268      42

  Fragments arranged by size: 

                 371     268      42
 
SmlI C'TyrA_G

Cuts at:      0     448     681 
   Size:        448     233
 
StyI C'CwwG_G

Cuts at:      0     610     619     681 
   Size:        610       9      62

  Fragments arranged by size: 

                 610      62       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     432     681 
   Size:        432     249
 
XmnI GAAnn'nnTTC

Cuts at:      0      33     668     681 
   Size:         33     635      13

  Fragments arranged by size: 

                 635      33      13
 

 Enzymes that do cut:

     ApaI     ApoI    AvrII    BanII     BbsI    BbvCI Bme1580I   Bpu10I
    BpuEI    BseYI     BsmI Bsp1286I    BsrDI    BssSI    BstBI     EarI
 EcoO109I    EcoRI    HaeII   MspA1I    PpuMI   PspOMI     PstI    PvuII
     SfcI     SmlI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI