(Linear) (Six-base) MAPSORT of: KIBB9802.seq  Check: 603  from: 1  to: 558



KIBB9802.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     354     558 
   Size:        354     204
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     558 
   Size:        352     206
 
AgeI A'CCGG_T

Cuts at:      0     527     558 
   Size:        527      31
 
ApoI r'AATT_y

Cuts at:      0      76     558 
   Size:         76     482
 
BamHI G'GATC_C

Cuts at:      0     264     558 
   Size:        264     294
 
BlpI GC'TnA_GC

Cuts at:      0     326     558 
   Size:        326     232
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     457     558 
   Size:        457     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     558 
   Size:        430     128
 
BsaWI w'CCGG_w

Cuts at:      0     527     558 
   Size:        527      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     552     558 
   Size:        382     170       6

  Fragments arranged by size: 

                 382     170       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     354     558 
   Size:        354     204
 
BsrFI r'CCGG_y

Cuts at:      0     527     558 
   Size:        527      31
 
BstYI r'GATC_y

Cuts at:      0     148     264     558 
   Size:        148     116     294

  Fragments arranged by size: 

                 294     148     116
 
Bsu36I CC'TnA_GG

Cuts at:      0      26     558 
   Size:         26     532
 
DraI TTT'AAA

Cuts at:      0     114     558 
   Size:        114     444
 
EaeI y'GGCC_r

Cuts at:      0     188     368     558 
   Size:        188     180     190

  Fragments arranged by size: 

                 190     188     180
 
EarI CTCTTCn'nnn_

Cuts at:      0      45     310     328     558 
   Size:         45     265      18     230

  Fragments arranged by size: 

                 265     230      45      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     430     558 
   Size:        352      78     128

  Fragments arranged by size: 

                 352     128      78
 
HincII GTy'rAC

Cuts at:      0     395     558 
   Size:        395     163
 
MscI TGG'CCA

Cuts at:      0     190     370     558 
   Size:        190     180     188

  Fragments arranged by size: 

                 190     188     180
 
MslI CAynn'nnrTG

Cuts at:      0     422     558 
   Size:        422     136
 
MspA1I CmG'CkG

Cuts at:      0     330     558 
   Size:        330     228
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     458     490     558 
   Size:        458      32      68

  Fragments arranged by size: 

                 458      68      32
 
PvuII CAG'CTG

Cuts at:      0     330     558 
   Size:        330     228
 
ScaI AGT'ACT

Cuts at:      0     457     558 
   Size:        457     101
 
StyI C'CwwG_G

Cuts at:      0     371     558 
   Size:        371     187
 
TatI w'GTAC_w

Cuts at:      0     455     558 
   Size:        455     103
 
XbaI T'CTAG_A

Cuts at:      0     124     558 
   Size:        124     434
 
XmnI GAAnn'nnTTC

Cuts at:      0     132     558 
   Size:        132     426
 

 Enzymes that do cut:

     AarI     AcuI     AgeI     ApoI    BamHI     BlpI     BplI     BpmI
    BsaWI     BsgI    BspMI    BsrFI    BstYI   Bsu36I     DraI     EaeI
     EarI  Eco57MI   HincII     MscI     MslI   MspA1I     PpiI    PvuII
     ScaI     StyI     TatI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI