(Linear) (Six-base) MAPSORT of: KIBB9801.seq  Check: 8929  from: 1  to: 555



KIBB9801.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     410     555 
   Size:        410     145
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     555 
   Size:        218     337
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      70     271     555 
   Size:         70     201     284

  Fragments arranged by size: 

                 284     201      70
 
BglI GCCn_nnn'nGGC

Cuts at:      0     476     555 
   Size:        476      79
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      22     555 
   Size:         22     533
 
BsaHI Gr'CG_yC

Cuts at:      0     418     555 
   Size:        418     137
 
BsaWI w'CCGG_w

Cuts at:      0     521     549     555 
   Size:        521      28       6

  Fragments arranged by size: 

                 521      28       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     308     338     555 
   Size:        308      30     217

  Fragments arranged by size: 

                 308     217      30
 
BseYI C'CCAG_C

Cuts at:      0      75     555 
   Size:         75     480
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     412     555 
   Size:        146     266     143

  Fragments arranged by size: 

                 266     146     143
 
BsmI GAATG_Cn'

Cuts at:      0     320     555 
   Size:        320     235
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     412     555 
   Size:        146     266     143

  Fragments arranged by size: 

                 266     146     143
 
BspEI T'CCGG_A

Cuts at:      0     521     555 
   Size:        521      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     410     555 
   Size:        410     145
 
BsrFI r'CCGG_y

Cuts at:      0     426     555 
   Size:        426     129
 
BsrGI T'GTAC_A

Cuts at:      0     458     555 
   Size:        458      97
 
BstYI r'GATC_y

Cuts at:      0     188     518     555 
   Size:        188     330      37

  Fragments arranged by size: 

                 330     188      37
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     273     555 
   Size:        273     282
 
EaeI y'GGCC_r

Cuts at:      0     366     428     555 
   Size:        366      62     127

  Fragments arranged by size: 

                 366     127      62
 
EarI CTCTTCn'nnn_

Cuts at:      0     236     555 
   Size:        236     319
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     555 
   Size:        218     337
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     183     555 
   Size:        183     372
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     308     340     510     542     555 
   Size:        308      32     170      32      13

  Fragments arranged by size: 

                 308     170      32      32      13
 
MscI TGG'CCA

Cuts at:      0     368     555 
   Size:        368     187
 
MspA1I CmG'CkG

Cuts at:      0      10     555 
   Size:         10     545
 
NaeI GCC'GGC

Cuts at:      0     428     555 
   Size:        428     127
 
NgoMIV G'CCGG_C

Cuts at:      0     426     555 
   Size:        426     129
 
PfoI T'CCnGG_A

Cuts at:      0     326     555 
   Size:        326     229
 
PstI C_TGCA'G

Cuts at:      0      15     407     555 
   Size:         15     392     148

  Fragments arranged by size: 

                 392     148      15
 
SfcI C'TryA_G

Cuts at:      0      11     403     555 
   Size:         11     392     152

  Fragments arranged by size: 

                 392     152      11
 
StuI AGG'CCT

Cuts at:      0     226     555 
   Size:        226     329
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     412     555 
   Size:        412     143
 
TatI w'GTAC_w

Cuts at:      0     458     555 
   Size:        458      97
 

 Enzymes that do cut:

     AarI     AcuI     BbsI     BglI     BsaI    BsaHI    BsaWI    BsaXI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrFI    BsrGI
    BstYI     DrdI     EaeI     EarI  Eco57MI     FalI    Hin4I     MscI
   MspA1I     NaeI   NgoMIV     PfoI     PstI     SfcI     StuI    TaqII
     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BspHI    BsrBI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI