Restriction map for
pF1KB9792
(Linear) (Six-base) MAPSORT of: KIBB9792.seq Check: 7010 from: 1 to: 630
KIBB9792.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 96 630
Size: 96 534
AfeI AGC'GCT
Cuts at: 0 238 358 630
Size: 238 120 272
Fragments arranged by size:
272 238 120
AflIII A'CryG_T
Cuts at: 0 275 630
Size: 275 355
AlwNI CAG_nnn'CTG
Cuts at: 0 42 147 630
Size: 42 105 483
Fragments arranged by size:
483 105 42
AvaI C'yCGr_G
Cuts at: 0 309 630
Size: 309 321
BanI G'GyrC_C
Cuts at: 0 304 630
Size: 304 326
BanII G_rGCy'C
Cuts at: 0 521 630
Size: 521 109
BbeI G_GCGC'C
Cuts at: 0 308 630
Size: 308 322
BbsI GAAGACnn'nnnn_
Cuts at: 0 583 630
Size: 583 47
Bme1580I G_kGCm'C
Cuts at: 0 316 487 630
Size: 316 171 143
Fragments arranged by size:
316 171 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 630
Size: 616 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 450 630
Size: 450 180
BsaAI yAC'GTr
Cuts at: 0 120 425 630
Size: 120 305 205
Fragments arranged by size:
305 205 120
BsaBI GATnn'nnATC
Cuts at: 0 61 630
Size: 61 569
BsaHI Gr'CG_yC
Cuts at: 0 305 385 630
Size: 305 80 245
Fragments arranged by size:
305 245 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 630
Size: 430 30 170
Fragments arranged by size:
430 170 30
BseYI C'CCAG_C
Cuts at: 0 42 147 630
Size: 42 105 483
Fragments arranged by size:
483 105 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 630
Size: 34 596
Bsp1286I G_dGCh'C
Cuts at: 0 316 487 521 630
Size: 316 171 34 109
Fragments arranged by size:
316 171 109 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 96 630
Size: 96 534
BssHII G'CGCG_C
Cuts at: 0 18 20 630
Size: 18 2 610
Fragments arranged by size:
610 18 2
BssSI C'ACGA_G
Cuts at: 0 507 630
Size: 507 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 12 630
Size: 12 618
BstYI r'GATC_y
Cuts at: 0 56 630
Size: 56 574
BtgI C'CryG_G
Cuts at: 0 581 630
Size: 581 49
DraIII CAC_nnn'GTG
Cuts at: 0 12 630
Size: 12 618
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 388 630
Size: 388 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 630
Size: 616 14
HaeII r_GCGC'y
Cuts at: 0 240 308 360 630
Size: 240 68 52 270
Fragments arranged by size:
270 240 68 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 292 294 324 630
Size: 262 30 2 30 306
Fragments arranged by size:
306 262 30 30 2
KasI G'GCGC_C
Cuts at: 0 304 630
Size: 304 326
MluI A'CGCG_T
Cuts at: 0 275 630
Size: 275 355
MspA1I CmG'CkG
Cuts at: 0 160 170 630
Size: 160 10 460
Fragments arranged by size:
460 160 10
NarI GG'CG_CC
Cuts at: 0 305 630
Size: 305 325
NcoI C'CATG_G
Cuts at: 0 581 630
Size: 581 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 78 494 630
Size: 42 36 416 136
Fragments arranged by size:
416 136 42 36
PmlI CAC'GTG
Cuts at: 0 120 630
Size: 120 510
SexAI A'CCwGG_T
Cuts at: 0 440 630
Size: 440 190
SfoI GGC'GCC
Cuts at: 0 306 630
Size: 306 324
StyI C'CwwG_G
Cuts at: 0 320 362 368 533 581 630
Size: 320 42 6 165 48 49
Fragments arranged by size:
320 165 49 48 42 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 630
Size: 333 297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 448 630
Size: 448 182
Enzymes that do cut:
AarI AfeI AflIII AlwNI AvaI BanI BanII BbeI
BbsI Bme1580I BpmI BsaI BsaAI BsaBI BsaHI BsaXI
BseYI BsgI Bsp1286I BspMI BssHII BssSI BstAPI BstYI
BtgI DraIII EciI Eco57MI HaeII Hin4I KasI MluI
MspA1I NarI NcoI PflMI PmlI SexAI SfoI StyI
TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MmeI MscI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI