Restriction map for
pF1KB9789
(Linear) (Six-base) MAPSORT of: KIBB9789.seq Check: 8141 from: 1 to: 606
KIBB9789.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AfeI AGC'GCT
Cuts at: 0 407 606
Size: 407 199
AflII C'TTAA_G
Cuts at: 0 409 606
Size: 409 197
AleI CACnn'nnGTG
Cuts at: 0 288 606
Size: 288 318
AlwNI CAG_nnn'CTG
Cuts at: 0 357 606
Size: 357 249
ApaI G_GGCC'C
Cuts at: 0 448 524 606
Size: 448 76 82
Fragments arranged by size:
448 82 76
AscI GG'CGCG_CC
Cuts at: 0 100 276 606
Size: 100 176 330
Fragments arranged by size:
330 176 100
AvaI C'yCGr_G
Cuts at: 0 317 496 606
Size: 317 179 110
Fragments arranged by size:
317 179 110
AvrII C'CTAG_G
Cuts at: 0 531 606
Size: 531 75
BanI G'GyrC_C
Cuts at: 0 77 466 606
Size: 77 389 140
Fragments arranged by size:
389 140 77
BanII G_rGCy'C
Cuts at: 0 188 419 448 524 606
Size: 188 231 29 76 82
Fragments arranged by size:
231 188 82 76 29
BbeI G_GCGC'C
Cuts at: 0 81 470 606
Size: 81 389 136
Fragments arranged by size:
389 136 81
BglI GCCn_nnn'nGGC
Cuts at: 0 170 452 464 606
Size: 170 282 12 142
Fragments arranged by size:
282 170 142 12
Bme1580I G_kGCm'C
Cuts at: 0 448 524 606
Size: 448 76 82
Fragments arranged by size:
448 82 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 388 531 606
Size: 43 345 143 75
Fragments arranged by size:
345 143 75 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 606
Size: 172 434
BsaHI Gr'CG_yC
Cuts at: 0 78 204 467 606
Size: 78 126 263 139
Fragments arranged by size:
263 139 126 78
BsaWI w'CCGG_w
Cuts at: 0 570 606
Size: 570 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 55 106 397 606
Size: 40 15 51 291 209
Fragments arranged by size:
291 209 51 40 15
BseYI C'CCAG_C
Cuts at: 0 429 460 606
Size: 429 31 146
Fragments arranged by size:
429 146 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 606
Size: 139 467
BsiEI CG_ry'CG
Cuts at: 0 129 606
Size: 129 477
BsiHKAI G_wGCw'C
Cuts at: 0 188 419 606
Size: 188 231 187
Fragments arranged by size:
231 188 187
Bsp1286I G_dGCh'C
Cuts at: 0 188 419 448 524 606
Size: 188 231 29 76 82
Fragments arranged by size:
231 188 82 76 29
BspEI T'CCGG_A
Cuts at: 0 570 606
Size: 570 36
BsrFI r'CCGG_y
Cuts at: 0 200 606
Size: 200 406
BssHII G'CGCG_C
Cuts at: 0 100 276 606
Size: 100 176 330
Fragments arranged by size:
330 176 100
BstYI r'GATC_y
Cuts at: 0 176 606
Size: 176 430
BtgI C'CryG_G
Cuts at: 0 450 606
Size: 450 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 551 606
Size: 551 55
EaeI y'GGCC_r
Cuts at: 0 126 359 606
Size: 126 233 247
Fragments arranged by size:
247 233 126
EagI C'GGCC_G
Cuts at: 0 126 606
Size: 126 480
EarI CTCTTCn'nnn_
Cuts at: 0 59 304 606
Size: 59 245 302
Fragments arranged by size:
302 245 59
EcoICRI GAG'CTC
Cuts at: 0 186 417 606
Size: 186 231 189
Fragments arranged by size:
231 189 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 388 531 606
Size: 43 345 143 75
Fragments arranged by size:
345 143 75 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 606
Size: 136 470
EcoO109I rG'GnC_Cy
Cuts at: 0 305 333 444 445 521 606
Size: 305 28 111 1 76 85
Fragments arranged by size:
305 111 85 76 28 1
HaeII r_GCGC'y
Cuts at: 0 55 81 264 409 430 470 606
Size: 55 26 183 145 21 40 136
Fragments arranged by size:
183 145 136 55 40 26 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 606
Size: 529 32 45
Fragments arranged by size:
529 45 32
KasI G'GCGC_C
Cuts at: 0 77 466 606
Size: 77 389 140
Fragments arranged by size:
389 140 77
MslI CAynn'nnrTG
Cuts at: 0 288 606
Size: 288 318
MspA1I CmG'CkG
Cuts at: 0 156 452 464 606
Size: 156 296 12 142
Fragments arranged by size:
296 156 142 12
NarI GG'CG_CC
Cuts at: 0 78 467 606
Size: 78 389 139
Fragments arranged by size:
389 139 78
NotI GC'GGCC_GC
Cuts at: 0 126 606
Size: 126 480
PfoI T'CCnGG_A
Cuts at: 0 248 606
Size: 248 358
PpuMI rG'GwC_Cy
Cuts at: 0 305 333 606
Size: 305 28 273
Fragments arranged by size:
305 273 28
PspOMI G'GGCC_C
Cuts at: 0 444 520 606
Size: 444 76 86
Fragments arranged by size:
444 86 76
PstI C_TGCA'G
Cuts at: 0 161 212 606
Size: 161 51 394
Fragments arranged by size:
394 161 51
PvuII CAG'CTG
Cuts at: 0 156 606
Size: 156 450
SacI G_AGCT'C
Cuts at: 0 188 419 606
Size: 188 231 187
Fragments arranged by size:
231 188 187
SacII CC_GC'GG
Cuts at: 0 453 606
Size: 453 153
SanDI GG'GwC_CC
Cuts at: 0 305 606
Size: 305 301
SapI GCTCTTCn'nnn_
Cuts at: 0 59 304 606
Size: 59 245 302
Fragments arranged by size:
302 245 59
SfcI C'TryA_G
Cuts at: 0 157 208 606
Size: 157 51 398
Fragments arranged by size:
398 157 51
SfoI GGC'GCC
Cuts at: 0 79 468 606
Size: 79 389 138
Fragments arranged by size:
389 138 79
SmaI CCC'GGG
Cuts at: 0 319 606
Size: 319 287
SmlI C'TyrA_G
Cuts at: 0 187 409 606
Size: 187 222 197
Fragments arranged by size:
222 197 187
StyI C'CwwG_G
Cuts at: 0 531 606
Size: 531 75
XmaI C'CCGG_G
Cuts at: 0 317 606
Size: 317 289
Enzymes that do cut:
AfeI AflII AleI AlwNI ApaI AscI AvaI AvrII
BanI BanII BbeI BglI Bme1580I BpmI BpuEI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BsrFI BssHII BstYI BtgI BtgZI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI
MspA1I NarI NotI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SapI SfcI SfoI SmaI SmlI
StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AseI AsiSI BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaXI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI