(Linear) (Six-base) MAPSORT of: KIBB9786.seq  Check: 835  from: 1  to: 573



KIBB9786.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AclI AA'CG_TT

Cuts at:      0     227     573 
   Size:        227     346
 
AlwNI CAG_nnn'CTG

Cuts at:      0     432     573 
   Size:        432     141
 
BanI G'GyrC_C

Cuts at:      0     340     380     402     573 
   Size:        340      40      22     171

  Fragments arranged by size: 

                 340     171      40      22
 
BanII G_rGCy'C

Cuts at:      0     363     573 
   Size:        363     210
 
BbeI G_GCGC'C

Cuts at:      0     384     406     573 
   Size:        384      22     167

  Fragments arranged by size: 

                 384     167      22
 
BlpI GC'TnA_GC

Cuts at:      0     471     573 
   Size:        471     102
 
Bme1580I G_kGCm'C

Cuts at:      0     326     343     573 
   Size:        326      17     230

  Fragments arranged by size: 

                 326     230      17
 
BmgBI CAC'GTC

Cuts at:      0      50     573 
   Size:         50     523
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     573 
   Size:        370     203
 
BsaHI Gr'CG_yC

Cuts at:      0     381     403     573 
   Size:        381      22     170

  Fragments arranged by size: 

                 381     170      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199     573 
   Size:        199     374
 
BseYI C'CCAG_C

Cuts at:      0     362     573 
   Size:        362     211
 
BsiHKAI G_wGCw'C

Cuts at:      0     530     573 
   Size:        530      43
 
BsmI GAATG_Cn'

Cuts at:      0     472     573 
   Size:        472     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     326     343     363     530     573 
   Size:        326      17      20     167      43

  Fragments arranged by size: 

                 326     167      43      20      17
 
BsrDI GCAATG_nn'

Cuts at:      0     248     573 
   Size:        248     325
 
BssHII G'CGCG_C

Cuts at:      0     122     573 
   Size:        122     451
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      14      50     573 
   Size:         14      36     523

  Fragments arranged by size: 

                 523      36      14
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     333     573 
   Size:        333     240
 
BstYI r'GATC_y

Cuts at:      0     443     573 
   Size:        443     130
 
BtsI GCAGTG_nn'

Cuts at:      0     170     309     573 
   Size:        170     139     264

  Fragments arranged by size: 

                 264     170     139
 
DraIII CAC_nnn'GTG

Cuts at:      0     453     573 
   Size:        453     120
 
EaeI y'GGCC_r

Cuts at:      0     434     573 
   Size:        434     139
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     248     573 
   Size:        248     325
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     481     573 
   Size:        481      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0     351     573 
   Size:        351     222
 
HaeII r_GCGC'y

Cuts at:      0     384     406     573 
   Size:        384      22     167

  Fragments arranged by size: 

                 384     167      22
 
HincII GTy'rAC

Cuts at:      0     138     573 
   Size:        138     435
 
KasI G'GCGC_C

Cuts at:      0     380     402     573 
   Size:        380      22     171

  Fragments arranged by size: 

                 380     171      22
 
MscI TGG'CCA

Cuts at:      0     436     573 
   Size:        436     137
 
NarI GG'CG_CC

Cuts at:      0     381     403     573 
   Size:        381      22     170

  Fragments arranged by size: 

                 381     170      22
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     432     573 
   Size:        432     141
 
PpuMI rG'GwC_Cy

Cuts at:      0     351     573 
   Size:        351     222
 
SfoI GGC'GCC

Cuts at:      0     382     404     573 
   Size:        382      22     169

  Fragments arranged by size: 

                 382     169      22
 
SmlI C'TyrA_G

Cuts at:      0     385     573 
   Size:        385     188
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      16     573 
   Size:         16     557
 
TatI w'GTAC_w

Cuts at:      0      92     573 
   Size:         92     481
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     333     573 
   Size:        333     240
 

 Enzymes that do cut:

     AclI    AlwNI     BanI    BanII     BbeI     BlpI Bme1580I    BmgBI
    BpuEI    BsaHI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI
   BssHII   BstAPI    BstXI    BstYI     BtsI   DraIII     EaeI     EciI
    EcoNI EcoO109I    HaeII   HincII     KasI     MscI     NarI    PflMI
    PpuMI     SfoI     SmlI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EarI
  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI