Restriction map for
pF1KB9784
(Linear) (Six-base) MAPSORT of: KIBB9784.seq Check: 7383 from: 1 to: 567
KIBB9784.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 462 530 567
Size: 462 68 37
Fragments arranged by size:
462 68 37
AlwNI CAG_nnn'CTG
Cuts at: 0 291 567
Size: 291 276
ApaI G_GGCC'C
Cuts at: 0 36 197 439 567
Size: 36 161 242 128
Fragments arranged by size:
242 161 128 36
AvaI C'yCGr_G
Cuts at: 0 61 96 137 567
Size: 61 35 41 430
Fragments arranged by size:
430 61 41 35
BanI G'GyrC_C
Cuts at: 0 26 562 567
Size: 26 536 5
Fragments arranged by size:
536 26 5
BanII G_rGCy'C
Cuts at: 0 36 156 197 439 567
Size: 36 120 41 242 128
Fragments arranged by size:
242 128 120 41 36
BbeI G_GCGC'C
Cuts at: 0 30 567
Size: 30 537
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 567
Size: 10 557
BglI GCCn_nnn'nGGC
Cuts at: 0 24 286 419 502 567
Size: 24 262 133 83 65
Fragments arranged by size:
262 133 83 65 24
Bme1580I G_kGCm'C
Cuts at: 0 36 197 439 565 567
Size: 36 161 242 126 2
Fragments arranged by size:
242 161 126 36 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 445 567
Size: 253 192 122
Fragments arranged by size:
253 192 122
BsaAI yAC'GTr
Cuts at: 0 312 567
Size: 312 255
BsaHI Gr'CG_yC
Cuts at: 0 27 73 567
Size: 27 46 494
Fragments arranged by size:
494 46 27
BseYI C'CCAG_C
Cuts at: 0 478 506 567
Size: 478 28 61
Fragments arranged by size:
478 61 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 567
Size: 61 506
BsiEI CG_ry'CG
Cuts at: 0 133 567
Size: 133 434
BsmBI CGTCTCn'nnnn_
Cuts at: 0 337 567
Size: 337 230
Bsp1286I G_dGCh'C
Cuts at: 0 36 156 197 439 565 567
Size: 36 120 41 242 126 2
Fragments arranged by size:
242 126 120 41 36 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 462 530 567
Size: 462 68 37
Fragments arranged by size:
462 68 37
BsrBI CCG'CTC
Cuts at: 0 252 567
Size: 252 315
BsrFI r'CCGG_y
Cuts at: 0 14 108 128 132 567
Size: 14 94 20 4 435
Fragments arranged by size:
435 94 20 14 4
BssHII G'CGCG_C
Cuts at: 0 315 567
Size: 315 252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 567
Size: 261 306
BstYI r'GATC_y
Cuts at: 0 542 567
Size: 542 25
BtgI C'CryG_G
Cuts at: 0 22 497 567
Size: 22 475 70
Fragments arranged by size:
475 70 22
EaeI y'GGCC_r
Cuts at: 0 110 130 178 201 356 383 567
Size: 110 20 48 23 155 27 184
Fragments arranged by size:
184 155 110 48 27 23 20
EagI C'GGCC_G
Cuts at: 0 130 567
Size: 130 437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 445 567
Size: 253 192 122
Fragments arranged by size:
253 192 122
EcoO109I rG'GnC_Cy
Cuts at: 0 33 83 184 288 436 567
Size: 33 50 101 104 148 131
Fragments arranged by size:
148 131 104 101 50 33
FseI GG_CCGG'CC
Cuts at: 0 136 567
Size: 136 431
HaeII r_GCGC'y
Cuts at: 0 30 567
Size: 30 537
KasI G'GCGC_C
Cuts at: 0 26 567
Size: 26 541
MscI TGG'CCA
Cuts at: 0 358 567
Size: 358 209
MspA1I CmG'CkG
Cuts at: 0 225 258 567
Size: 225 33 309
Fragments arranged by size:
309 225 33
NaeI GCC'GGC
Cuts at: 0 110 130 134 567
Size: 110 20 4 433
Fragments arranged by size:
433 110 20 4
NarI GG'CG_CC
Cuts at: 0 27 567
Size: 27 540
NgoMIV G'CCGG_C
Cuts at: 0 108 128 132 567
Size: 108 20 4 435
Fragments arranged by size:
435 108 20 4
PasI CC'CwG_GG
Cuts at: 0 513 567
Size: 513 54
PmlI CAC'GTG
Cuts at: 0 312 567
Size: 312 255
PpuMI rG'GwC_Cy
Cuts at: 0 83 184 567
Size: 83 101 383
Fragments arranged by size:
383 101 83
PspOMI G'GGCC_C
Cuts at: 0 32 193 435 567
Size: 32 161 242 132
Fragments arranged by size:
242 161 132 32
PstI C_TGCA'G
Cuts at: 0 527 567
Size: 527 40
SanDI GG'GwC_CC
Cuts at: 0 83 567
Size: 83 484
SfcI C'TryA_G
Cuts at: 0 523 567
Size: 523 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 502 567
Size: 502 65
SfoI GGC'GCC
Cuts at: 0 28 567
Size: 28 539
SmaI CCC'GGG
Cuts at: 0 63 98 139 567
Size: 63 35 41 428
Fragments arranged by size:
428 63 41 35
SrfI GCCC'GGGC
Cuts at: 0 139 567
Size: 139 428
StuI AGG'CCT
Cuts at: 0 422 567
Size: 422 145
StyI C'CwwG_G
Cuts at: 0 113 567
Size: 113 454
Tth111I GACn'n_nGTC
Cuts at: 0 399 567
Size: 399 168
XmaI C'CCGG_G
Cuts at: 0 61 96 137 567
Size: 61 35 41 430
Fragments arranged by size:
430 61 41 35
Enzymes that do cut:
AarI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BglI Bme1580I BpmI BsaAI BsaHI BseYI BsgI BsiEI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI
BtgI EaeI EagI Eco57MI EcoO109I FseI HaeII KasI
MscI MspA1I NaeI NarI NgoMIV PasI PmlI PpuMI
PspOMI PstI SanDI SfcI SfiI SfoI SmaI SrfI
StuI StyI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiHKAI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI
TaqII TatI TstI XbaI XcmI XhoI XmnI ZraI