Restriction map for
pF1KB9783
(Linear) (Six-base) MAPSORT of: KIBB9783.seq Check: 2304 from: 1 to: 753
KIBB9783.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 15 645 753
Size: 15 630 108
Fragments arranged by size:
630 108 15
AatII G_ACGT'C
Cuts at: 0 545 753
Size: 545 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 753
Size: 608 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 753
Size: 280 473
AlwNI CAG_nnn'CTG
Cuts at: 0 249 753
Size: 249 504
ApaI G_GGCC'C
Cuts at: 0 388 482 623 753
Size: 388 94 141 130
Fragments arranged by size:
388 141 130 94
BanI G'GyrC_C
Cuts at: 0 363 393 753
Size: 363 30 360
Fragments arranged by size:
363 360 30
BanII G_rGCy'C
Cuts at: 0 274 340 388 482 515 623 753
Size: 274 66 48 94 33 108 130
Fragments arranged by size:
274 130 108 94 66 48 33
BbvCI CC'TCA_GC
Cuts at: 0 719 753
Size: 719 34
BglI GCCn_nnn'nGGC
Cuts at: 0 326 477 610 753
Size: 326 151 133 143
Fragments arranged by size:
326 151 143 133
Bme1580I G_kGCm'C
Cuts at: 0 322 366 388 398 482 623 753
Size: 322 44 22 10 84 141 130
Fragments arranged by size:
322 141 130 84 44 22 10
BmgBI CAC'GTC
Cuts at: 0 317 753
Size: 317 436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 753
Size: 508 245
Bpu10I CC'TnA_GC
Cuts at: 0 368 719 753
Size: 368 351 34
Fragments arranged by size:
368 351 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 633 753
Size: 633 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 182 728 753
Size: 182 546 25
Fragments arranged by size:
546 182 25
BsaAI yAC'GTr
Cuts at: 0 122 753
Size: 122 631
BsaHI Gr'CG_yC
Cuts at: 0 542 753
Size: 542 211
BsaWI w'CCGG_w
Cuts at: 0 47 753
Size: 47 706
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 626 656 753
Size: 626 30 97
Fragments arranged by size:
626 97 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 196 607 753
Size: 187 9 411 146
Fragments arranged by size:
411 187 146 9
BsiHKAI G_wGCw'C
Cuts at: 0 140 274 515 753
Size: 140 134 241 238
Fragments arranged by size:
241 238 140 134
Bsp1286I G_dGCh'C
Cuts at: 0 140 274 322 340 366 388 398 482
Size: 140 134 48 18 26 22 10 84
Cuts at: 482 515 623 753
Size: 33 108 130
Fragments arranged by size:
140 134 130 108 84 48 33 26
22 18 10
BspEI T'CCGG_A
Cuts at: 0 47 753
Size: 47 706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 272 452 645 753
Size: 15 257 180 193 108
Fragments arranged by size:
257 193 180 108 15
BsrBI CCG'CTC
Cuts at: 0 414 753
Size: 414 339
BsrFI r'CCGG_y
Cuts at: 0 531 753
Size: 531 222
BsrGI T'GTAC_A
Cuts at: 0 251 753
Size: 251 502
BstEII G'GTnAC_C
Cuts at: 0 657 753
Size: 657 96
Bsu36I CC'TnA_GG
Cuts at: 0 341 753
Size: 341 412
BtgI C'CryG_G
Cuts at: 0 35 474 753
Size: 35 439 279
Fragments arranged by size:
439 279 35
BtsI GCAGTG_nn'
Cuts at: 0 747 753
Size: 747 6
EaeI y'GGCC_r
Cuts at: 0 611 753
Size: 611 142
EarI CTCTTCn'nnn_
Cuts at: 0 128 205 520 584 753
Size: 128 77 315 64 169
Fragments arranged by size:
315 169 128 77 64
EcoICRI GAG'CTC
Cuts at: 0 272 513 753
Size: 272 241 240
Fragments arranged by size:
272 241 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 608 753
Size: 508 100 145
Fragments arranged by size:
508 145 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 753
Size: 613 140
EcoO109I rG'GnC_Cy
Cuts at: 0 384 385 619 620 753
Size: 384 1 234 1 133
Fragments arranged by size:
384 234 133 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 321 405 753
Size: 321 84 348
Fragments arranged by size:
348 321 84
FspI TGC'GCA
Cuts at: 0 578 753
Size: 578 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 497 529 753
Size: 181 32 284 32 224
Fragments arranged by size:
284 224 181 32 32
MspA1I CmG'CkG
Cuts at: 0 246 332 476 718 753
Size: 246 86 144 242 35
Fragments arranged by size:
246 242 144 86 35
PmlI CAC'GTG
Cuts at: 0 122 753
Size: 122 631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 753
Size: 13 32 708
Fragments arranged by size:
708 32 13
PspOMI G'GGCC_C
Cuts at: 0 384 478 619 753
Size: 384 94 141 134
Fragments arranged by size:
384 141 134 94
PstI C_TGCA'G
Cuts at: 0 245 524 551 753
Size: 245 279 27 202
Fragments arranged by size:
279 245 202 27
PvuII CAG'CTG
Cuts at: 0 718 753
Size: 718 35
SacI G_AGCT'C
Cuts at: 0 274 515 753
Size: 274 241 238
Fragments arranged by size:
274 241 238
SacII CC_GC'GG
Cuts at: 0 477 753
Size: 477 276
SapI GCTCTTCn'nnn_
Cuts at: 0 128 520 753
Size: 128 392 233
Fragments arranged by size:
392 233 128
SbfI CC_TGCA'GG
Cuts at: 0 524 551 753
Size: 524 27 202
Fragments arranged by size:
524 202 27
SfcI C'TryA_G
Cuts at: 0 241 520 547 753
Size: 241 279 27 206
Fragments arranged by size:
279 241 206 27
SmlI C'TyrA_G
Cuts at: 0 648 753
Size: 648 105
StyI C'CwwG_G
Cuts at: 0 284 389 753
Size: 284 105 364
Fragments arranged by size:
364 284 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 206 682 753
Size: 206 476 71
Fragments arranged by size:
476 206 71
TatI w'GTAC_w
Cuts at: 0 113 251 753
Size: 113 138 502
Fragments arranged by size:
502 138 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 626 658 753
Size: 626 32 95
Fragments arranged by size:
626 95 32
ZraI GAC'GTC
Cuts at: 0 543 753
Size: 543 210
Enzymes that do cut:
AarI AatII AcuI AlfI AlwNI ApaI BanI BanII
BbvCI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BstEII Bsu36I BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
Hin4I MspA1I PmlI PpiI PspOMI PstI PvuII SacI
SacII SapI SbfI SfcI SmlI StyI TaqII TatI
TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI BsaBI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrDI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV
FseI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI