Restriction map for
pF1KB9782
(Linear) (Six-base) MAPSORT of: KIBB9782.seq Check: 1011 from: 1 to: 627
KIBB9782.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 397 627
Size: 304 93 230
Fragments arranged by size:
304 230 93
ApaI G_GGCC'C
Cuts at: 0 250 593 627
Size: 250 343 34
Fragments arranged by size:
343 250 34
ApaLI G'TGCA_C
Cuts at: 0 298 627
Size: 298 329
BanI G'GyrC_C
Cuts at: 0 237 383 565 627
Size: 237 146 182 62
Fragments arranged by size:
237 182 146 62
BanII G_rGCy'C
Cuts at: 0 250 442 593 627
Size: 250 192 151 34
Fragments arranged by size:
250 192 151 34
BbeI G_GCGC'C
Cuts at: 0 387 627
Size: 387 240
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 627
Size: 158 469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 383 627
Size: 349 34 244
Fragments arranged by size:
349 244 34
BlpI GC'TnA_GC
Cuts at: 0 274 627
Size: 274 353
Bme1580I G_kGCm'C
Cuts at: 0 242 250 302 336 593 627
Size: 242 8 52 34 257 34
Fragments arranged by size:
257 242 52 34 34 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 220 627
Size: 27 193 407
Fragments arranged by size:
407 193 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 67 334 409 627
Size: 49 18 267 75 218
Fragments arranged by size:
267 218 75 49 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 238 627
Size: 211 27 389
Fragments arranged by size:
389 211 27
BsaHI Gr'CG_yC
Cuts at: 0 384 627
Size: 384 243
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 627
Size: 382 30 215
Fragments arranged by size:
382 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 627
Size: 331 296
BseYI C'CCAG_C
Cuts at: 0 606 627
Size: 606 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 627
Size: 424 203
BsiEI CG_ry'CG
Cuts at: 0 38 134 627
Size: 38 96 493
Fragments arranged by size:
493 96 38
BsiHKAI G_wGCw'C
Cuts at: 0 74 302 627
Size: 74 228 325
Fragments arranged by size:
325 228 74
Bsp1286I G_dGCh'C
Cuts at: 0 74 242 250 302 336 442 593 627
Size: 74 168 8 52 34 106 151 34
Fragments arranged by size:
168 151 106 74 52 34 34 8
BsrFI r'CCGG_y
Cuts at: 0 133 568 599 627
Size: 133 435 31 28
Fragments arranged by size:
435 133 31 28
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 627
Size: 237 390
BtgI C'CryG_G
Cuts at: 0 452 627
Size: 452 175
BtsI GCAGTG_nn'
Cuts at: 0 537 594 627
Size: 537 57 33
Fragments arranged by size:
537 57 33
EaeI y'GGCC_r
Cuts at: 0 35 131 627
Size: 35 96 496
Fragments arranged by size:
496 96 35
EagI C'GGCC_G
Cuts at: 0 35 131 627
Size: 35 96 496
Fragments arranged by size:
496 96 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 67 304 334 397 409 627
Size: 49 18 237 30 63 12 218
Fragments arranged by size:
237 218 63 49 30 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 627
Size: 28 599
EcoO109I rG'GnC_Cy
Cuts at: 0 150 247 590 627
Size: 150 97 343 37
Fragments arranged by size:
343 150 97 37
FseI GG_CCGG'CC
Cuts at: 0 137 627
Size: 137 490
FspI TGC'GCA
Cuts at: 0 145 424 627
Size: 145 279 203
Fragments arranged by size:
279 203 145
FspAI rTGC'GCAy
Cuts at: 0 424 627
Size: 424 203
HaeII r_GCGC'y
Cuts at: 0 387 627
Size: 387 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 627
Size: 382 32 213
Fragments arranged by size:
382 213 32
KasI G'GCGC_C
Cuts at: 0 383 627
Size: 383 244
MspA1I CmG'CkG
Cuts at: 0 354 396 627
Size: 354 42 231
Fragments arranged by size:
354 231 42
NaeI GCC'GGC
Cuts at: 0 135 601 627
Size: 135 466 26
Fragments arranged by size:
466 135 26
NarI GG'CG_CC
Cuts at: 0 384 627
Size: 384 243
NgoMIV G'CCGG_C
Cuts at: 0 133 599 627
Size: 133 466 28
Fragments arranged by size:
466 133 28
PasI CC'CwG_GG
Cuts at: 0 243 627
Size: 243 384
PspOMI G'GGCC_C
Cuts at: 0 246 589 627
Size: 246 343 38
Fragments arranged by size:
343 246 38
PstI C_TGCA'G
Cuts at: 0 359 560 627
Size: 359 201 67
Fragments arranged by size:
359 201 67
PvuII CAG'CTG
Cuts at: 0 354 396 627
Size: 354 42 231
Fragments arranged by size:
354 231 42
SfcI C'TryA_G
Cuts at: 0 355 549 556 627
Size: 355 194 7 71
Fragments arranged by size:
355 194 71 7
SfoI GGC'GCC
Cuts at: 0 385 627
Size: 385 242
SgrAI Cr'CCGG_yG
Cuts at: 0 568 627
Size: 568 59
SmlI C'TyrA_G
Cuts at: 0 217 226 627
Size: 217 9 401
Fragments arranged by size:
401 217 9
StyI C'CwwG_G
Cuts at: 0 293 627
Size: 293 334
Enzymes that do cut:
AcuI ApaI ApaLI BanI BanII BbeI BbsI BcgI
BlpI Bme1580I BplI BpmI BpuEI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BstAPI BtgI
BtsI EaeI EagI Eco57MI EcoNI EcoO109I FseI FspI
FspAI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV
PasI PspOMI PstI PvuII SfcI SfoI SgrAI SmlI
StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I
BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FalI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI