Restriction map for
pF1KB9775
(Linear) (Six-base) MAPSORT of: KIBB9775.seq Check: 9861 from: 1 to: 2034
KIBB9775.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1988 2034
Size: 1988 46
AatII G_ACGT'C
Cuts at: 0 296 2034
Size: 296 1738
AccI GT'mk_AC
Cuts at: 0 275 2034
Size: 275 1759
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 275 443 511 1135 1737 2034
Size: 223 52 168 68 624 602 297
Fragments arranged by size:
624 602 297 223 168 68 52
AfeI AGC'GCT
Cuts at: 0 40 1531 2034
Size: 40 1491 503
Fragments arranged by size:
1491 503 40
AflII C'TTAA_G
Cuts at: 0 789 2034
Size: 789 1245
AlwNI CAG_nnn'CTG
Cuts at: 0 57 354 489 1023 1306 1344 1596 2034
Size: 57 297 135 534 283 38 252 438
Fragments arranged by size:
534 438 297 283 252 135 57 38
ApoI r'AATT_y
Cuts at: 0 415 987 2034
Size: 415 572 1047
Fragments arranged by size:
1047 572 415
BanI G'GyrC_C
Cuts at: 0 849 1384 1471 2034
Size: 849 535 87 563
Fragments arranged by size:
849 563 535 87
BanII G_rGCy'C
Cuts at: 0 1421 2034
Size: 1421 613
BbsI GAAGACnn'nnnn_
Cuts at: 0 1887 2034
Size: 1887 147
BbvCI CC'TCA_GC
Cuts at: 0 1813 2034
Size: 1813 221
BclI T'GATC_A
Cuts at: 0 725 2034
Size: 725 1309
BglII A'GATC_T
Cuts at: 0 1001 2034
Size: 1001 1033
BlpI GC'TnA_GC
Cuts at: 0 611 1024 1391 2034
Size: 611 413 367 643
Fragments arranged by size:
643 611 413 367
Bme1580I G_kGCm'C
Cuts at: 0 1476 1542 2034
Size: 1476 66 492
Fragments arranged by size:
1476 492 66
BmgBI CAC'GTC
Cuts at: 0 858 948 2034
Size: 858 90 1086
Fragments arranged by size:
1086 858 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 1713 2034
Size: 1713 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 319 622 808 873 943 1195 1210
Size: 152 167 303 186 65 70 252 15
Cuts at: 1210 1354 1423 1500 1573 2034
Size: 144 69 77 73 461
Fragments arranged by size:
461 303 252 186 167 152 144 77
73 70 69 65 15
Bpu10I CC'TnA_GC
Cuts at: 0 1249 1345 1783 1813 2034
Size: 1249 96 438 30 221
Fragments arranged by size:
1249 438 221 96 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1033 1576 1809 2034
Size: 538 495 543 233 225
Fragments arranged by size:
543 538 495 233 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 2034
Size: 778 1256
BsaHI Gr'CG_yC
Cuts at: 0 293 1824 2034
Size: 293 1531 210
Fragments arranged by size:
1531 293 210
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 317 347 1168 1198 1693 1723 1729 1759
Size: 317 30 821 30 495 30 6 30
Cuts at: 1759 2034
Size: 275
Fragments arranged by size:
821 495 317 275 30 30 30 30
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1737 1741 2034
Size: 1737 4 293
Fragments arranged by size:
1737 293 4
BseYI C'CCAG_C
Cuts at: 0 1147 1577 2034
Size: 1147 430 457
Fragments arranged by size:
1147 457 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 367 970 1283 1867 1966 2034
Size: 340 27 603 313 584 99 68
Fragments arranged by size:
603 584 340 313 99 68 27
BsiHKAI G_wGCw'C
Cuts at: 0 1397 1907 2034
Size: 1397 510 127
Fragments arranged by size:
1397 510 127
Bsp1286I G_dGCh'C
Cuts at: 0 1397 1421 1476 1542 1907 2034
Size: 1397 24 55 66 365 127
Fragments arranged by size:
1397 365 127 66 55 24
BspHI T'CATG_A
Cuts at: 0 728 2034
Size: 728 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 177 1461 1988 2034
Size: 177 1284 527 46
Fragments arranged by size:
1284 527 177 46
BsrBI CCG'CTC
Cuts at: 0 887 921 1202 1253 1767 2034
Size: 887 34 281 51 514 267
Fragments arranged by size:
887 514 281 267 51 34
BsrFI r'CCGG_y
Cuts at: 0 98 2034
Size: 98 1936
BssHII G'CGCG_C
Cuts at: 0 1620 1849 2034
Size: 1620 229 185
Fragments arranged by size:
1620 229 185
BssSI C'ACGA_G
Cuts at: 0 1993 2034
Size: 1993 41
BstAPI GCAn_nnn'nTGC
Cuts at: 0 354 2034
Size: 354 1680
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1173 1845 2034
Size: 1173 672 189
Fragments arranged by size:
1173 672 189
BstYI r'GATC_y
Cuts at: 0 300 318 839 1001 1409 2034
Size: 300 18 521 162 408 625
Fragments arranged by size:
625 521 408 300 162 18
BtgI C'CryG_G
Cuts at: 0 1121 2034
Size: 1121 913
BtsI GCAGTG_nn'
Cuts at: 0 1433 1498 1695 1884 2034
Size: 1433 65 197 189 150
Fragments arranged by size:
1433 197 189 150 65
ClaI AT'CG_AT
Cuts at: 0 19 2034
Size: 19 2015
DrdI GACnn_nn'nnGTC
Cuts at: 0 582 2034
Size: 582 1452
EaeI y'GGCC_r
Cuts at: 0 5 809 908 992 1163 1295 1674 2034
Size: 5 804 99 84 171 132 379 360
Fragments arranged by size:
804 379 360 171 132 99 84 5
EarI CTCTTCn'nnn_
Cuts at: 0 199 1082 2034
Size: 199 883 952
Fragments arranged by size:
952 883 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 796 2034
Size: 796 1238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 223 275 319 443 511 622 808
Size: 152 71 52 44 124 68 111 186
Cuts at: 808 873 943 1135 1195 1210 1354 1423 1500
Size: 65 70 192 60 15 144 69 77
Cuts at: 1500 1573 1737 2034
Size: 73 164 297
Fragments arranged by size:
297 192 186 164 152 144 124 111
77 73 71 70 69 68 65 60
52 44 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1336 1480 2034
Size: 1336 144 554
Fragments arranged by size:
1336 554 144
EcoO109I rG'GnC_Cy
Cuts at: 0 1341 1593 2034
Size: 1341 252 441
Fragments arranged by size:
1341 441 252
EcoRI G'AATT_C
Cuts at: 0 987 2034
Size: 987 1047
FspI TGC'GCA
Cuts at: 0 181 1231 2034
Size: 181 1050 803
Fragments arranged by size:
1050 803 181
HaeII r_GCGC'y
Cuts at: 0 42 196 354 1031 1148 1533 1734 2034
Size: 42 154 158 677 117 385 201 300
Fragments arranged by size:
677 385 300 201 158 154 117 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 317 349 1307 1339 1729 1761 1776 1808
Size: 317 32 958 32 390 32 15 32
Cuts at: 1808 2034
Size: 226
Fragments arranged by size:
958 390 317 226 32 32 32 32
15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1262 1862 2034
Size: 1262 600 172
Fragments arranged by size:
1262 600 172
MscI TGG'CCA
Cuts at: 0 1297 2034
Size: 1297 737
MslI CAynn'nnrTG
Cuts at: 0 874 1997 2034
Size: 874 1123 37
Fragments arranged by size:
1123 874 37
MspA1I CmG'CkG
Cuts at: 0 57 198 828 936 1023 1368 1390 1469
Size: 57 141 630 108 87 345 22 79
Cuts at: 1469 1685 2034
Size: 216 349
Fragments arranged by size:
630 349 345 216 141 108 87 79
57 22
NcoI C'CATG_G
Cuts at: 0 1121 2034
Size: 1121 913
PasI CC'CwG_GG
Cuts at: 0 1796 2034
Size: 1796 238
PpuMI rG'GwC_Cy
Cuts at: 0 1341 2034
Size: 1341 693
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 518 550 2034
Size: 518 32 1484
Fragments arranged by size:
1484 518 32
PstI C_TGCA'G
Cuts at: 0 62 572 1211 1458 1601 1664 2034
Size: 62 510 639 247 143 63 370
Fragments arranged by size:
639 510 370 247 143 63 62
PvuII CAG'CTG
Cuts at: 0 57 828 936 1023 2034
Size: 57 771 108 87 1011
Fragments arranged by size:
1011 771 108 87 57
SfcI C'TryA_G
Cuts at: 0 58 568 1207 1454 1597 1660 2034
Size: 58 510 639 247 143 63 374
Fragments arranged by size:
639 510 374 247 143 63 58
SmlI C'TyrA_G
Cuts at: 0 517 789 1012 1555 1788 2034
Size: 517 272 223 543 233 246
Fragments arranged by size:
543 517 272 246 233 223
SspI AAT'ATT
Cuts at: 0 31 2034
Size: 31 2003
StyI C'CwwG_G
Cuts at: 0 213 914 1121 2034
Size: 213 701 207 913
Fragments arranged by size:
913 701 213 207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 597 1815 2034
Size: 597 1218 219
Fragments arranged by size:
1218 597 219
XmnI GAAnn'nnTTC
Cuts at: 0 208 2034
Size: 208 1826
ZraI GAC'GTC
Cuts at: 0 294 2034
Size: 294 1740
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AlwNI ApoI
BanI BanII BbsI BbvCI BclI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI
ClaI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I MmeI MscI MslI MspA1I
NcoI PasI PpuMI PsrI PstI PvuII SfcI SmlI
SspI StyI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflIII AgeI AhdI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BciVI BfrBI BglI BmtI BplI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI
DraI DraIII EagI EcoICRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI