Restriction map for
pF1KB9766
(Linear) (Six-base) MAPSORT of: KIBB9766.seq Check: 9894 from: 1 to: 2799
KIBB9766.orf
With 182 enzymes: SgfI *
August 26, 2010 17:36 ..
AccI GT'mk_AC
Cuts at: 0 1366 1610 2799
Size: 1366 244 1189
Fragments arranged by size:
1366 1189 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 158 290 730 2750 2799
Size: 31 127 132 440 2020 49
Fragments arranged by size:
2020 440 132 127 49 31
AflIII A'CryG_T
Cuts at: 0 1115 2307 2799
Size: 1115 1192 492
Fragments arranged by size:
1192 1115 492
AhdI GACnn_n'nnGTC
Cuts at: 0 231 2799
Size: 231 2568
AleI CACnn'nnGTG
Cuts at: 0 81 895 2312 2799
Size: 81 814 1417 487
Fragments arranged by size:
1417 814 487 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 665 2799
Size: 665 2134
AlwNI CAG_nnn'CTG
Cuts at: 0 237 585 969 2325 2799
Size: 237 348 384 1356 474
Fragments arranged by size:
1356 474 384 348 237
ApaI G_GGCC'C
Cuts at: 0 624 1420 2799
Size: 624 796 1379
Fragments arranged by size:
1379 796 624
ApoI r'AATT_y
Cuts at: 0 1912 2710 2799
Size: 1912 798 89
Fragments arranged by size:
1912 798 89
AscI GG'CGCG_CC
Cuts at: 0 1400 2799
Size: 1400 1399
AvaI C'yCGr_G
Cuts at: 0 26 61 108 370 557 722 871 1300
Size: 26 35 47 262 187 165 149 429
Cuts at: 1300 1359 2693 2799
Size: 59 1334 106
Fragments arranged by size:
1334 429 262 187 165 149 106 59
47 35 26
BamHI G'GATC_C
Cuts at: 0 70 2799
Size: 70 2729
BanI G'GyrC_C
Cuts at: 0 357 419 867 1222 1443 2799
Size: 357 62 448 355 221 1356
Fragments arranged by size:
1356 448 357 355 221 62
BanII G_rGCy'C
Cuts at: 0 33 607 624 729 1106 1420 1559 1663
Size: 33 574 17 105 377 314 139 104
Cuts at: 1663 2555 2599 2799
Size: 892 44 200
Fragments arranged by size:
892 574 377 314 200 139 105 104
44 33 17
BbeI G_GCGC'C
Cuts at: 0 361 871 1447 2799
Size: 361 510 576 1352
Fragments arranged by size:
1352 576 510 361
BbsI GAAGACnn'nnnn_
Cuts at: 0 980 1758 2799
Size: 980 778 1041
Fragments arranged by size:
1041 980 778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 676 710 2799
Size: 676 34 2089
Fragments arranged by size:
2089 676 34
BclI T'GATC_A
Cuts at: 0 2060 2799
Size: 2060 739
BglI GCCn_nnn'nGGC
Cuts at: 0 428 946 1516 1519 2799
Size: 428 518 570 3 1280
Fragments arranged by size:
1280 570 518 428 3
Bme1580I G_kGCm'C
Cuts at: 0 624 1420 2700 2799
Size: 624 796 1280 99
Fragments arranged by size:
1280 796 624 99
BmgBI CAC'GTC
Cuts at: 0 246 2310 2799
Size: 246 2064 489
Fragments arranged by size:
2064 489 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 619 2511 2799
Size: 619 1892 288
Fragments arranged by size:
1892 619 288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 693 2799
Size: 693 2106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 767 1393 2115 2799
Size: 308 459 626 722 684
Fragments arranged by size:
722 684 626 459 308
Bpu10I CC'TnA_GC
Cuts at: 0 2005 2799
Size: 2005 794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1573 2799
Size: 1573 1226
BsaAI yAC'GTr
Cuts at: 0 39 915 2799
Size: 39 876 1884
Fragments arranged by size:
1884 876 39
BsaBI GATnn'nnATC
Cuts at: 0 2295 2799
Size: 2295 504
BsaHI Gr'CG_yC
Cuts at: 0 358 791 868 1097 1444 2799
Size: 358 433 77 229 347 1355
Fragments arranged by size:
1355 433 358 347 229 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2518 2548 2799
Size: 2518 30 251
Fragments arranged by size:
2518 251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 679 682 703 764 767 1180 1183 1347
Size: 679 3 21 61 3 413 3 164
Cuts at: 1347 2539 2799
Size: 1192 260
Fragments arranged by size:
1192 679 413 260 164 61 21 3
3 3
BseYI C'CCAG_C
Cuts at: 0 90 385 470 1409 1857 2799
Size: 90 295 85 939 448 942
Fragments arranged by size:
942 939 448 295 90 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1523 2726 2799
Size: 1523 1203 73
Fragments arranged by size:
1523 1203 73
BsiEI CG_ry'CG
Cuts at: 0 1058 1322 2799
Size: 1058 264 1477
Fragments arranged by size:
1477 1058 264
BsiHKAI G_wGCw'C
Cuts at: 0 1589 1950 2555 2599 2799
Size: 1589 361 605 44 200
Fragments arranged by size:
1589 605 361 200 44
BsmBI CGTCTCn'nnnn_
Cuts at: 0 641 2799
Size: 641 2158
Bsp1286I G_dGCh'C
Cuts at: 0 33 607 624 729 1106 1420 1559 1589
Size: 33 574 17 105 377 314 139 30
Cuts at: 1589 1663 1950 2555 2599 2700 2799
Size: 74 287 605 44 101 99
Fragments arranged by size:
605 574 377 314 287 139 105 101
99 74 44 33 30 17
BspHI T'CATG_A
Cuts at: 0 491 2799
Size: 491 2308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 88 1596 2799
Size: 88 1508 1203
Fragments arranged by size:
1508 1203 88
BsrBI CCG'CTC
Cuts at: 0 489 1150 1457 2799
Size: 489 661 307 1342
Fragments arranged by size:
1342 661 489 307
BsrDI GCAATG_nn'
Cuts at: 0 1787 2203 2799
Size: 1787 416 596
Fragments arranged by size:
1787 596 416
BsrFI r'CCGG_y
Cuts at: 0 453 497 1219 2799
Size: 453 44 722 1580
Fragments arranged by size:
1580 722 453 44
BsrGI T'GTAC_A
Cuts at: 0 1385 2799
Size: 1385 1414
BssHII G'CGCG_C
Cuts at: 0 1192 1400 2799
Size: 1192 208 1399
Fragments arranged by size:
1399 1192 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 1954 2799
Size: 585 1369 845
Fragments arranged by size:
1369 845 585
BstBI TT'CG_AA
Cuts at: 0 2217 2799
Size: 2217 582
BstEII G'GTnAC_C
Cuts at: 0 405 2799
Size: 405 2394
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 2760 2799
Size: 900 1860 39
Fragments arranged by size:
1860 900 39
BstYI r'GATC_y
Cuts at: 0 70 447 2296 2408 2799
Size: 70 377 1849 112 391
Fragments arranged by size:
1849 391 377 112 70
Bsu36I CC'TnA_GG
Cuts at: 0 363 1633 2799
Size: 363 1270 1166
Fragments arranged by size:
1270 1166 363
BtgI C'CryG_G
Cuts at: 0 758 893 1024 1471 2799
Size: 758 135 131 447 1328
Fragments arranged by size:
1328 758 447 135 131
BtsI GCAGTG_nn'
Cuts at: 0 76 2799
Size: 76 2723
DraI TTT'AAA
Cuts at: 0 1917 2799
Size: 1917 882
DraIII CAC_nnn'GTG
Cuts at: 0 1039 2704 2799
Size: 1039 1665 95
Fragments arranged by size:
1665 1039 95
EaeI y'GGCC_r
Cuts at: 0 146 887 1217 1319 2799
Size: 146 741 330 102 1480
Fragments arranged by size:
1480 741 330 146 102
EagI C'GGCC_G
Cuts at: 0 1319 2799
Size: 1319 1480
EarI CTCTTCn'nnn_
Cuts at: 0 181 2441 2799
Size: 181 2260 358
Fragments arranged by size:
2260 358 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1485 2799
Size: 1485 1314
EcoICRI GAG'CTC
Cuts at: 0 2553 2597 2799
Size: 2553 44 202
Fragments arranged by size:
2553 202 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 158 290 308 730 767 1393 2115
Size: 31 127 132 18 422 37 626 722
Cuts at: 2115 2750 2799
Size: 635 49
Fragments arranged by size:
722 635 626 422 132 127 49 37
31 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1603 2495 2799
Size: 1603 892 304
Fragments arranged by size:
1603 892 304
EcoO109I rG'GnC_Cy
Cuts at: 0 22 201 367 561 620 621 1416 1596
Size: 22 179 166 194 59 1 795 180
Cuts at: 1596 2799
Size: 1203
Fragments arranged by size:
1203 795 194 180 179 166 59 22
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1785 2799
Size: 1785 1014
HaeII r_GCGC'y
Cuts at: 0 361 832 871 879 1013 1447 2799
Size: 361 471 39 8 134 434 1352
Fragments arranged by size:
1352 471 434 361 134 39 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 217 249 1705 1737 2518 2540
Size: 55 32 130 32 1456 32 781 22
Cuts at: 2540 2550 2572 2799
Size: 10 22 227
Fragments arranged by size:
1456 781 227 130 55 32 32 32
22 22 10
HincII GTy'rAC
Cuts at: 0 1367 2799
Size: 1367 1432
HindIII A'AGCT_T
Cuts at: 0 2428 2799
Size: 2428 371
KasI G'GCGC_C
Cuts at: 0 357 867 1443 2799
Size: 357 510 576 1356
Fragments arranged by size:
1356 576 510 357
MfeI C'AATT_G
Cuts at: 0 2564 2799
Size: 2564 235
MluI A'CGCG_T
Cuts at: 0 1115 2799
Size: 1115 1684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 221 233 495 2799
Size: 48 173 12 262 2304
Fragments arranged by size:
2304 262 173 48 12
MscI TGG'CCA
Cuts at: 0 889 2799
Size: 889 1910
MslI CAynn'nnrTG
Cuts at: 0 81 895 898 913 1736 2231 2312 2799
Size: 81 814 3 15 823 495 81 487
Fragments arranged by size:
823 814 495 487 81 81 15 3
MspA1I CmG'CkG
Cuts at: 0 323 474 538 582 649 760 885 966
Size: 323 151 64 44 67 111 125 81
Cuts at: 966 1026 1272 1473 2361 2799
Size: 60 246 201 888 438
Fragments arranged by size:
888 438 323 246 201 151 125 111
81 67 64 60 44
NarI GG'CG_CC
Cuts at: 0 358 868 1444 2799
Size: 358 510 576 1355
Fragments arranged by size:
1355 576 510 358
NspI r_CATG'y
Cuts at: 0 1865 2799
Size: 1865 934
PasI CC'CwG_GG
Cuts at: 0 833 2799
Size: 833 1966
PflMI CCAn_nnn'nTGG
Cuts at: 0 416 2799
Size: 416 2383
PfoI T'CCnGG_A
Cuts at: 0 526 1241 2799
Size: 526 715 1558
Fragments arranged by size:
1558 715 526
PmlI CAC'GTG
Cuts at: 0 39 915 2799
Size: 39 876 1884
Fragments arranged by size:
1884 876 39
PpuMI rG'GwC_Cy
Cuts at: 0 22 201 367 2799
Size: 22 179 166 2432
Fragments arranged by size:
2432 179 166 22
PspOMI G'GGCC_C
Cuts at: 0 620 1416 2799
Size: 620 796 1383
Fragments arranged by size:
1383 796 620
PvuII CAG'CTG
Cuts at: 0 538 582 966 2799
Size: 538 44 384 1833
Fragments arranged by size:
1833 538 384 44
RsrII CG'GwC_CG
Cuts at: 0 500 2799
Size: 500 2299
SacI G_AGCT'C
Cuts at: 0 2555 2599 2799
Size: 2555 44 200
Fragments arranged by size:
2555 200 44
SacII CC_GC'GG
Cuts at: 0 761 1027 1474 2799
Size: 761 266 447 1325
Fragments arranged by size:
1325 761 447 266
SalI G'TCGA_C
Cuts at: 0 1365 2799
Size: 1365 1434
SanDI GG'GwC_CC
Cuts at: 0 22 2799
Size: 22 2777
SexAI A'CCwGG_T
Cuts at: 0 2028 2799
Size: 2028 771
SfcI C'TryA_G
Cuts at: 0 1061 1128 2799
Size: 1061 67 1671
Fragments arranged by size:
1671 1061 67
SfoI GGC'GCC
Cuts at: 0 359 869 1445 2799
Size: 359 510 576 1354
Fragments arranged by size:
1354 576 510 359
SmaI CCC'GGG
Cuts at: 0 28 110 372 559 873 1302 2695 2799
Size: 28 82 262 187 314 429 1393 104
Fragments arranged by size:
1393 429 314 262 187 104 82 28
SmlI C'TyrA_G
Cuts at: 0 1588 2799
Size: 1588 1211
SrfI GCCC'GGGC
Cuts at: 0 873 2799
Size: 873 1926
StuI AGG'CCT
Cuts at: 0 1186 2799
Size: 1186 1613
StyI C'CwwG_G
Cuts at: 0 806 1106 1899 2799
Size: 806 300 793 900
Fragments arranged by size:
900 806 793 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 484 721 2799
Size: 484 237 2078
Fragments arranged by size:
2078 484 237
TatI w'GTAC_w
Cuts at: 0 1385 2666 2799
Size: 1385 1281 133
Fragments arranged by size:
1385 1281 133
Tth111I GACn'n_nGTC
Cuts at: 0 337 2799
Size: 337 2462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 897 2799
Size: 840 57 1902
Fragments arranged by size:
1902 840 57
XmaI C'CCGG_G
Cuts at: 0 26 108 370 557 871 1300 2693 2799
Size: 26 82 262 187 314 429 1393 106
Fragments arranged by size:
1393 429 314 262 187 106 82 26
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AscI AvaI BamHI BanI BanII BbeI BbsI
BcgI BclI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HindIII KasI MfeI MluI MmeI MscI
MslI MspA1I NarI NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PvuII RsrII SacI SacII SalI SanDI
SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApaLI AseI AsiSI AvrII BaeI BbvCI BciVI BfrBI
BglII BlpI BmtI BsaI BsaWI BsiWI BsmI BspEI
BssSI BstZ17I BtgZI ClaI CspCI DrdI EcoRI EcoRV
FseI FspI FspAI HpaI KpnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TstI XbaI XhoI XmnI ZraI