Restriction map for
pF1KB9765
(Linear) (Six-base) MAPSORT of: KIBB9765.seq Check: 2198 from: 1 to: 2709
KIBB9765.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 0 2378 2660 2709
Size: 2378 282 49
Fragments arranged by size:
2378 282 49
AclI AA'CG_TT
Cuts at: 0 48 2709
Size: 48 2661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 2709
Size: 1046 1663
AfeI AGC'GCT
Cuts at: 0 1585 2709
Size: 1585 1124
AflIII A'CryG_T
Cuts at: 0 1424 2549 2612 2709
Size: 1424 1125 63 97
Fragments arranged by size:
1424 1125 97 63
AhdI GACnn_n'nnGTC
Cuts at: 0 1302 2148 2709
Size: 1302 846 561
Fragments arranged by size:
1302 846 561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2329 2458 2709
Size: 2329 129 251
Fragments arranged by size:
2329 251 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1339 1371 2709
Size: 1339 32 1338
Fragments arranged by size:
1339 1338 32
AlwNI CAG_nnn'CTG
Cuts at: 0 381 1901 2691 2709
Size: 381 1520 790 18
Fragments arranged by size:
1520 790 381 18
ApaLI G'TGCA_C
Cuts at: 0 302 326 736 2326 2536 2709
Size: 302 24 410 1590 210 173
Fragments arranged by size:
1590 410 302 210 173 24
ApoI r'AATT_y
Cuts at: 0 418 2709
Size: 418 2291
AseI AT'TA_AT
Cuts at: 0 1157 2709
Size: 1157 1552
AvaI C'yCGr_G
Cuts at: 0 77 634 914 2188 2709
Size: 77 557 280 1274 521
Fragments arranged by size:
1274 557 521 280 77
AvrII C'CTAG_G
Cuts at: 0 453 2709
Size: 453 2256
BamHI G'GATC_C
Cuts at: 0 30 2709
Size: 30 2679
BanI G'GyrC_C
Cuts at: 0 3 1754 2068 2104 2389 2709
Size: 3 1751 314 36 285 320
Fragments arranged by size:
1751 320 314 285 36 3
BanII G_rGCy'C
Cuts at: 0 1403 1777 1808 2709
Size: 1403 374 31 901
Fragments arranged by size:
1403 901 374 31
BbeI G_GCGC'C
Cuts at: 0 7 1758 2072 2108 2709
Size: 7 1751 314 36 601
Fragments arranged by size:
1751 601 314 36 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1034 1397 2709
Size: 1034 363 1312
Fragments arranged by size:
1312 1034 363
BbvCI CC'TCA_GC
Cuts at: 0 587 2709
Size: 587 2122
BciVI GTATCCnnnnn_n'
Cuts at: 0 1124 2709
Size: 1124 1585
BglI GCCn_nnn'nGGC
Cuts at: 0 1781 1812 2119 2709
Size: 1781 31 307 590
Fragments arranged by size:
1781 590 307 31
BglII A'GATC_T
Cuts at: 0 1862 1925 2709
Size: 1862 63 784
Fragments arranged by size:
1862 784 63
BlpI GC'TnA_GC
Cuts at: 0 530 603 2709
Size: 530 73 2106
Fragments arranged by size:
2106 530 73
Bme1580I G_kGCm'C
Cuts at: 0 306 330 583 740 2330 2392 2540 2709
Size: 306 24 253 157 1590 62 148 169
Fragments arranged by size:
1590 306 253 169 157 148 62 24
BmgBI CAC'GTC
Cuts at: 0 1032 1637 2709
Size: 1032 605 1072
Fragments arranged by size:
1072 1032 605
BmrI ACTGGGnnnn_n'
Cuts at: 0 317 653 2279 2709
Size: 317 336 1626 430
Fragments arranged by size:
1626 430 336 317
BmtI G_CTAG'C
Cuts at: 0 2588 2709
Size: 2588 121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1326 2709
Size: 1326 1383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1452 1674 1914 2299 2709
Size: 1452 222 240 385 410
Fragments arranged by size:
1452 410 385 240 222
Bpu10I CC'TnA_GC
Cuts at: 0 587 2709
Size: 587 2122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1831 2709
Size: 706 1125 878
Fragments arranged by size:
1125 878 706
BsaI GGTCTCn'nnnn_
Cuts at: 0 571 622 844 2709
Size: 571 51 222 1865
Fragments arranged by size:
1865 571 222 51
BsaAI yAC'GTr
Cuts at: 0 519 1590 2034 2535 2709
Size: 519 1071 444 501 174
Fragments arranged by size:
1071 519 501 444 174
BsaBI GATnn'nnATC
Cuts at: 0 1570 2709
Size: 1570 1139
BsaHI Gr'CG_yC
Cuts at: 0 4 1755 2069 2105 2167 2509 2709
Size: 4 1751 314 36 62 342 200
Fragments arranged by size:
1751 342 314 200 62 36 4
BsaWI w'CCGG_w
Cuts at: 0 1406 2709
Size: 1406 1303
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 503 533 1333 1363 1585 1615 2709
Size: 503 30 800 30 222 30 1094
Fragments arranged by size:
1094 800 503 222 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 606 2709
Size: 606 2103
BseYI C'CCAG_C
Cuts at: 0 16 537 823 1966 2242 2709
Size: 16 521 286 1143 276 467
Fragments arranged by size:
1143 521 467 286 276 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2014 2709
Size: 2014 695
BsiEI CG_ry'CG
Cuts at: 0 1407 2084 2366 2627 2709
Size: 1407 677 282 261 82
Fragments arranged by size:
1407 677 282 261 82
BsiHKAI G_wGCw'C
Cuts at: 0 306 330 740 1511 1557 1847 1979 2318
Size: 306 24 410 771 46 290 132 339
Cuts at: 2318 2330 2540 2709
Size: 12 210 169
Fragments arranged by size:
771 410 339 306 290 210 169 132
46 24 12
BsmI GAATG_Cn'
Cuts at: 0 1173 2709
Size: 1173 1536
Bsp1286I G_dGCh'C
Cuts at: 0 306 330 583 740 1403 1511 1557 1777
Size: 306 24 253 157 663 108 46 220
Cuts at: 1777 1808 1847 1979 2318 2330 2392 2540 2709
Size: 31 39 132 339 12 62 148 169
Fragments arranged by size:
663 339 306 253 220 169 157 148
132 108 62 46 39 31 24 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1616 2447 2709
Size: 1616 831 262
Fragments arranged by size:
1616 831 262
BsrBI CCG'CTC
Cuts at: 0 2160 2709
Size: 2160 549
BsrFI r'CCGG_y
Cuts at: 0 779 1751 2540 2605 2709
Size: 779 972 789 65 104
Fragments arranged by size:
972 789 779 104 65
BsrGI T'GTAC_A
Cuts at: 0 425 2264 2709
Size: 425 1839 445
Fragments arranged by size:
1839 445 425
BssHII G'CGCG_C
Cuts at: 0 1679 2709
Size: 1679 1030
BssSI C'ACGA_G
Cuts at: 0 841 2709
Size: 841 1868
BstAPI GCAn_nnn'nTGC
Cuts at: 0 861 2295 2709
Size: 861 1434 414
Fragments arranged by size:
1434 861 414
BstEII G'GTnAC_C
Cuts at: 0 2334 2415 2709
Size: 2334 81 294
Fragments arranged by size:
2334 294 81
BstYI r'GATC_y
Cuts at: 0 30 972 1862 1925 2709
Size: 30 942 890 63 784
Fragments arranged by size:
942 890 784 63 30
Bsu36I CC'TnA_GG
Cuts at: 0 1323 2709
Size: 1323 1386
BtgI C'CryG_G
Cuts at: 0 247 807 1235 1810 2117 2173 2384 2513
Size: 247 560 428 575 307 56 211 129
Cuts at: 2513 2621 2709
Size: 108 88
Fragments arranged by size:
575 560 428 307 247 211 129 108
88 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1227 1579 2709
Size: 1227 352 1130
Fragments arranged by size:
1227 1130 352
BtsI GCAGTG_nn'
Cuts at: 0 492 581 2579 2709
Size: 492 89 1998 130
Fragments arranged by size:
1998 492 130 89
DraIII CAC_nnn'GTG
Cuts at: 0 2334 2535 2709
Size: 2334 201 174
Fragments arranged by size:
2334 201 174
EaeI y'GGCC_r
Cuts at: 0 131 1458 2036 2081 2111 2348 2363 2423
Size: 131 1327 578 45 30 237 15 60
Cuts at: 2423 2624 2709
Size: 201 85
Fragments arranged by size:
1327 578 237 201 131 85 60 45
30 15
EagI C'GGCC_G
Cuts at: 0 2081 2363 2624 2709
Size: 2081 282 261 85
Fragments arranged by size:
2081 282 261 85
EarI CTCTTCn'nnn_
Cuts at: 0 152 994 2641 2709
Size: 152 842 1647 68
Fragments arranged by size:
1647 842 152 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1734 2709
Size: 1734 975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1452 1674 1914 2299 2709
Size: 1046 406 222 240 385 410
Fragments arranged by size:
1046 410 406 385 240 222
EcoNI CCTnn'n_nnAGG
Cuts at: 0 574 2709
Size: 574 2135
EcoO109I rG'GnC_Cy
Cuts at: 0 240 268 2274 2342 2709
Size: 240 28 2006 68 367
Fragments arranged by size:
2006 367 240 68 28
EcoRV GAT'ATC
Cuts at: 0 990 2709
Size: 990 1719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 2709
Size: 36 2673
FspI TGC'GCA
Cuts at: 0 854 2709
Size: 854 1855
HaeII r_GCGC'y
Cuts at: 0 7 1587 1649 1745 1758 2072 2108 2287
Size: 7 1580 62 96 13 314 36 179
Cuts at: 2287 2709
Size: 422
Fragments arranged by size:
1580 422 314 179 96 62 36 13
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1288 1320 1585 1617 2134 2166
Size: 166 32 1090 32 265 32 517 32
Cuts at: 2166 2709
Size: 543
Fragments arranged by size:
1090 543 517 265 166 32 32 32
32
HincII GTy'rAC
Cuts at: 0 650 2463 2478 2709
Size: 650 1813 15 231
Fragments arranged by size:
1813 650 231 15
HpaI GTT'AAC
Cuts at: 0 2463 2478 2709
Size: 2463 15 231
Fragments arranged by size:
2463 231 15
KasI G'GCGC_C
Cuts at: 0 3 1754 2068 2104 2709
Size: 3 1751 314 36 605
Fragments arranged by size:
1751 605 314 36 3
MluI A'CGCG_T
Cuts at: 0 1424 2709
Size: 1424 1285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1319 1382 1583 1664 2165 2276 2709
Size: 605 714 63 201 81 501 111 433
Fragments arranged by size:
714 605 501 433 201 111 81 63
MscI TGG'CCA
Cuts at: 0 133 2038 2425 2709
Size: 133 1905 387 284
Fragments arranged by size:
1905 387 284 133
MslI CAynn'nnrTG
Cuts at: 0 706 1192 1234 2518 2709
Size: 706 486 42 1284 191
Fragments arranged by size:
1284 706 486 191 42
MspA1I CmG'CkG
Cuts at: 0 537 1812 2119 2148 2175 2322 2709
Size: 537 1275 307 29 27 147 387
Fragments arranged by size:
1275 537 387 307 147 29 27
NaeI GCC'GGC
Cuts at: 0 781 1753 2607 2709
Size: 781 972 854 102
Fragments arranged by size:
972 854 781 102
NarI GG'CG_CC
Cuts at: 0 4 1755 2069 2105 2709
Size: 4 1751 314 36 604
Fragments arranged by size:
1751 604 314 36 4
NcoI C'CATG_G
Cuts at: 0 1235 2513 2709
Size: 1235 1278 196
Fragments arranged by size:
1278 1235 196
NdeI CA'TA_TG
Cuts at: 0 902 2709
Size: 902 1807
NgoMIV G'CCGG_C
Cuts at: 0 779 1751 2605 2709
Size: 779 972 854 104
Fragments arranged by size:
972 854 779 104
NheI G'CTAG_C
Cuts at: 0 2584 2709
Size: 2584 125
NotI GC'GGCC_GC
Cuts at: 0 2081 2709
Size: 2081 628
NspI r_CATG'y
Cuts at: 0 859 2553 2709
Size: 859 1694 156
Fragments arranged by size:
1694 859 156
PasI CC'CwG_GG
Cuts at: 0 556 2709
Size: 556 2153
PciI A'CATG_T
Cuts at: 0 2549 2709
Size: 2549 160
PflMI CCAn_nnn'nTGG
Cuts at: 0 834 2709
Size: 834 1875
PfoI T'CCnGG_A
Cuts at: 0 1930 2709
Size: 1930 779
PmlI CAC'GTG
Cuts at: 0 519 2034 2535 2709
Size: 519 1515 501 174
Fragments arranged by size:
1515 519 501 174
PpuMI rG'GwC_Cy
Cuts at: 0 240 2274 2342 2709
Size: 240 2034 68 367
Fragments arranged by size:
2034 367 240 68
PspXI vC'TCGA_Gb
Cuts at: 0 914 2709
Size: 914 1795
PstI C_TGCA'G
Cuts at: 0 156 1619 2291 2709
Size: 156 1463 672 418
Fragments arranged by size:
1463 672 418 156
PvuII CAG'CTG
Cuts at: 0 537 2709
Size: 537 2172
SacII CC_GC'GG
Cuts at: 0 1813 2120 2176 2709
Size: 1813 307 56 533
Fragments arranged by size:
1813 533 307 56
SanDI GG'GwC_CC
Cuts at: 0 240 2342 2709
Size: 240 2102 367
Fragments arranged by size:
2102 367 240
ScaI AGT'ACT
Cuts at: 0 1093 2709
Size: 1093 1616
SfcI C'TryA_G
Cuts at: 0 152 983 1615 1947 2287 2661 2709
Size: 152 831 632 332 340 374 48
Fragments arranged by size:
831 632 374 340 332 152 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2119 2709
Size: 2119 590
SfoI GGC'GCC
Cuts at: 0 5 1756 2070 2106 2709
Size: 5 1751 314 36 603
Fragments arranged by size:
1751 603 314 36 5
SgrAI Cr'CCGG_yG
Cuts at: 0 1751 2709
Size: 1751 958
SmaI CCC'GGG
Cuts at: 0 2190 2709
Size: 2190 519
SmlI C'TyrA_G
Cuts at: 0 685 914 1846 2709
Size: 685 229 932 863
Fragments arranged by size:
932 863 685 229
SphI G_CATG'C
Cuts at: 0 859 2709
Size: 859 1850
StuI AGG'CCT
Cuts at: 0 458 1687 2709
Size: 458 1229 1022
Fragments arranged by size:
1229 1022 458
StyI C'CwwG_G
Cuts at: 0 453 1235 1529 1649 2513 2709
Size: 453 782 294 120 864 196
Fragments arranged by size:
864 782 453 294 196 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1182 1248 2040 2709
Size: 1182 66 792 669
Fragments arranged by size:
1182 792 669 66
TatI w'GTAC_w
Cuts at: 0 425 1091 1220 2264 2709
Size: 425 666 129 1044 445
Fragments arranged by size:
1044 666 445 425 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 503 535 746 778 1969 2001
Size: 232 32 239 32 211 32 1191 32
Cuts at: 2001 2709
Size: 708
Fragments arranged by size:
1191 708 239 232 211 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 499 2050 2709
Size: 499 1551 659
Fragments arranged by size:
1551 659 499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 831 2121 2511 2709
Size: 831 1290 390 198
Fragments arranged by size:
1290 831 390 198
XhoI C'TCGA_G
Cuts at: 0 914 2709
Size: 914 1795
XmaI C'CCGG_G
Cuts at: 0 2188 2709
Size: 2188 521
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AhdI AlfI AloI
AlwNI ApaLI ApoI AseI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmlI PpuMI PspXI PstI PvuII
SacII SanDI ScaI SfcI SfiI SfoI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AleI ApaI AscI
AsiSI BaeI BcgI BclI BfrBI BsiWI BsmBI BspEI
BspHI BsrDI BstBI BstXI BstZ17I ClaI CspCI DraI
DrdI EcoICRI EcoRI FseI FspAI HindIII KpnI MfeI
NruI NsiI PacI PmeI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacI SalI SapI SbfI SexAI
SgfI SnaBI SpeI SrfI SspI SwaI XbaI XmnI
ZraI