Restriction map for
pF1KB9758
(Linear) (Six-base) MAPSORT of: KIBB9758.seq Check: 4125 from: 1 to: 2133
KIBB9758.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 841 1063 1182 2133
Size: 841 222 119 951
Fragments arranged by size:
951 841 222 119
AccI GT'mk_AC
Cuts at: 0 230 281 2133
Size: 230 51 1852
Fragments arranged by size:
1852 230 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1018 1108 2133
Size: 563 455 90 1025
Fragments arranged by size:
1025 563 455 90
AfeI AGC'GCT
Cuts at: 0 1262 2133
Size: 1262 871
AflIII A'CryG_T
Cuts at: 0 1483 2133
Size: 1483 650
AleI CACnn'nnGTG
Cuts at: 0 89 521 1033 1457 2026 2133
Size: 89 432 512 424 569 107
Fragments arranged by size:
569 512 432 424 107 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 764 2133
Size: 764 1369
AlwNI CAG_nnn'CTG
Cuts at: 0 856 2133
Size: 856 1277
ApaLI G'TGCA_C
Cuts at: 0 1108 2133
Size: 1108 1025
ApoI r'AATT_y
Cuts at: 0 423 1364 1593 2133
Size: 423 941 229 540
Fragments arranged by size:
941 540 423 229
BamHI G'GATC_C
Cuts at: 0 1543 2133
Size: 1543 590
BanI G'GyrC_C
Cuts at: 0 1217 1321 2133
Size: 1217 104 812
Fragments arranged by size:
1217 812 104
BbeI G_GCGC'C
Cuts at: 0 1325 2133
Size: 1325 808
BbsI GAAGACnn'nnnn_
Cuts at: 0 569 732 2133
Size: 569 163 1401
Fragments arranged by size:
1401 569 163
BbvCI CC'TCA_GC
Cuts at: 0 752 2133
Size: 752 1381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 86 2133
Size: 52 34 2047
Fragments arranged by size:
2047 52 34
BfrBI ATG'CAT
Cuts at: 0 63 2133
Size: 63 2070
BglI GCCn_nnn'nGGC
Cuts at: 0 148 490 1235 2011 2133
Size: 148 342 745 776 122
Fragments arranged by size:
776 745 342 148 122
BlpI GC'TnA_GC
Cuts at: 0 2068 2133
Size: 2068 65
Bme1580I G_kGCm'C
Cuts at: 0 811 1112 2133
Size: 811 301 1021
Fragments arranged by size:
1021 811 301
BmgBI CAC'GTC
Cuts at: 0 96 1173 2133
Size: 96 1077 960
Fragments arranged by size:
1077 960 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 515 2133
Size: 515 1618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 2133
Size: 1669 464
Bpu10I CC'TnA_GC
Cuts at: 0 752 2133
Size: 752 1381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1333 2133
Size: 292 1041 800
Fragments arranged by size:
1041 800 292
BsaI GGTCTCn'nnnn_
Cuts at: 0 532 1286 2133
Size: 532 754 847
Fragments arranged by size:
847 754 532
BsaAI yAC'GTr
Cuts at: 0 1107 1185 2133
Size: 1107 78 948
Fragments arranged by size:
1107 948 78
BsaHI Gr'CG_yC
Cuts at: 0 1322 2133
Size: 1322 811
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 651 681 856 886 2133
Size: 651 30 175 30 1247
Fragments arranged by size:
1247 651 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 907 916 919 922 925 928 2133
Size: 907 9 3 3 3 3 1205
Fragments arranged by size:
1205 907 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 679 1586 1670 2133
Size: 679 907 84 463
Fragments arranged by size:
907 679 463 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 265 337 975 1255 1293 2133
Size: 32 233 72 638 280 38 840
Fragments arranged by size:
840 638 280 233 72 38 32
BsiHKAI G_wGCw'C
Cuts at: 0 1112 2133
Size: 1112 1021
BsmI GAATG_Cn'
Cuts at: 0 329 2133
Size: 329 1804
Bsp1286I G_dGCh'C
Cuts at: 0 811 1112 2133
Size: 811 301 1021
Fragments arranged by size:
1021 811 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 237 813 841 1063 1182 2133
Size: 237 576 28 222 119 951
Fragments arranged by size:
951 576 237 222 119 28
BsrBI CCG'CTC
Cuts at: 0 1215 2133
Size: 1215 918
BsrDI GCAATG_nn'
Cuts at: 0 573 1737 2133
Size: 573 1164 396
Fragments arranged by size:
1164 573 396
BsrFI r'CCGG_y
Cuts at: 0 1295 2133
Size: 1295 838
BssSI C'ACGA_G
Cuts at: 0 1273 2133
Size: 1273 860
BstAPI GCAn_nnn'nTGC
Cuts at: 0 60 324 962 1188 1316 1736 2133
Size: 60 264 638 226 128 420 397
Fragments arranged by size:
638 420 397 264 226 128 60
BstEII G'GTnAC_C
Cuts at: 0 82 2133
Size: 82 2051
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1035 2133
Size: 1035 1098
BstYI r'GATC_y
Cuts at: 0 701 1543 2133
Size: 701 842 590
Fragments arranged by size:
842 701 590
BtgI C'CryG_G
Cuts at: 0 1017 1804 2024 2133
Size: 1017 787 220 109
Fragments arranged by size:
1017 787 220 109
BtsI GCAGTG_nn'
Cuts at: 0 158 284 462 2133
Size: 158 126 178 1671
Fragments arranged by size:
1671 178 158 126
DraI TTT'AAA
Cuts at: 0 1557 2133
Size: 1557 576
DraIII CAC_nnn'GTG
Cuts at: 0 1023 1316 2133
Size: 1023 293 817
Fragments arranged by size:
1023 817 293
DrdI GACnn_nn'nnGTC
Cuts at: 0 100 2133
Size: 100 2033
EaeI y'GGCC_r
Cuts at: 0 500 1025 1059 1227 1932 2048 2133
Size: 500 525 34 168 705 116 85
Fragments arranged by size:
705 525 500 168 116 85 34
EarI CTCTTCn'nnn_
Cuts at: 0 196 893 934 982 1084 1772 1874 2133
Size: 196 697 41 48 102 688 102 259
Fragments arranged by size:
697 688 259 196 102 102 48 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1018 1108 1669 2133
Size: 563 455 90 561 464
Fragments arranged by size:
563 561 464 455 90
EcoO109I rG'GnC_Cy
Cuts at: 0 1242 2133
Size: 1242 891
EcoRI G'AATT_C
Cuts at: 0 1364 1593 2133
Size: 1364 229 540
Fragments arranged by size:
1364 540 229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 208 963 1530 2133
Size: 208 755 567 603
Fragments arranged by size:
755 603 567 208
HaeII r_GCGC'y
Cuts at: 0 1104 1125 1264 1325 1692 2133
Size: 1104 21 139 61 367 441
Fragments arranged by size:
1104 441 367 139 61 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 856 888 1873 1905 2133
Size: 856 32 985 32 228
Fragments arranged by size:
985 856 228 32 32
HindIII A'AGCT_T
Cuts at: 0 1897 2133
Size: 1897 236
KasI G'GCGC_C
Cuts at: 0 1321 2133
Size: 1321 812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 2133
Size: 842 1291
MscI TGG'CCA
Cuts at: 0 502 1027 1934 2050 2133
Size: 502 525 907 116 83
Fragments arranged by size:
907 525 502 116 83
MslI CAynn'nnrTG
Cuts at: 0 89 521 814 1033 1457 1725 2026 2133
Size: 89 432 293 219 424 268 301 107
Fragments arranged by size:
432 424 301 293 268 219 107 89
MspA1I CmG'CkG
Cuts at: 0 1046 1079 1223 1590 2133
Size: 1046 33 144 367 543
Fragments arranged by size:
1046 543 367 144 33
NarI GG'CG_CC
Cuts at: 0 1322 2133
Size: 1322 811
NcoI C'CATG_G
Cuts at: 0 1804 2133
Size: 1804 329
NsiI A_TGCA'T
Cuts at: 0 65 2133
Size: 65 2068
NspI r_CATG'y
Cuts at: 0 576 1431 1487 2133
Size: 576 855 56 646
Fragments arranged by size:
855 646 576 56
PasI CC'CwG_GG
Cuts at: 0 488 2133
Size: 488 1645
PciI A'CATG_T
Cuts at: 0 1483 2133
Size: 1483 650
PflMI CCAn_nnn'nTGG
Cuts at: 0 1023 1947 2133
Size: 1023 924 186
Fragments arranged by size:
1023 924 186
PmlI CAC'GTG
Cuts at: 0 1107 1185 2133
Size: 1107 78 948
Fragments arranged by size:
1107 948 78
PshAI GACnn'nnGTC
Cuts at: 0 226 2133
Size: 226 1907
PstI C_TGCA'G
Cuts at: 0 1193 1956 2133
Size: 1193 763 177
Fragments arranged by size:
1193 763 177
SapI GCTCTTCn'nnn_
Cuts at: 0 934 982 1084 2133
Size: 934 48 102 1049
Fragments arranged by size:
1049 934 102 48
ScaI AGT'ACT
Cuts at: 0 407 1513 2133
Size: 407 1106 620
Fragments arranged by size:
1106 620 407
SfcI C'TryA_G
Cuts at: 0 771 1189 1952 2133
Size: 771 418 763 181
Fragments arranged by size:
771 763 418 181
SfoI GGC'GCC
Cuts at: 0 1323 2133
Size: 1323 810
SmlI C'TyrA_G
Cuts at: 0 271 1348 2133
Size: 271 1077 785
Fragments arranged by size:
1077 785 271
StyI C'CwwG_G
Cuts at: 0 539 602 1804 2133
Size: 539 63 1202 329
Fragments arranged by size:
1202 539 329 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1684 2133
Size: 1684 449
TatI w'GTAC_w
Cuts at: 0 405 1511 2133
Size: 405 1106 622
Fragments arranged by size:
1106 622 405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 651 683 760 792 2133
Size: 651 32 77 32 1341
Fragments arranged by size:
1341 651 77 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 2133
Size: 495 1638
XmnI GAAnn'nnTTC
Cuts at: 0 991 1153 1813 2133
Size: 991 162 660 320
Fragments arranged by size:
991 660 320 162
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaLI ApoI BamHI BanI BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI DraI DraIII
DrdI EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI NsiI NspI PasI PciI PflMI PmlI PshAI
PstI SapI ScaI SfcI SfoI SmlI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BanII BciVI
BclI BglII BmtI BplI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI PacI PfoI
PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI