Restriction map for
pF1KB9756
(Linear) (Six-base) MAPSORT of: KIBB9756.seq Check: 8221 from: 1 to: 2115
KIBB9756.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 806 1961 2115
Size: 806 1155 154
Fragments arranged by size:
1155 806 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1344 1376 1705 1880 2072 2115
Size: 1344 32 329 175 192 43
Fragments arranged by size:
1344 329 192 175 43 32
AfeI AGC'GCT
Cuts at: 0 96 273 2115
Size: 96 177 1842
Fragments arranged by size:
1842 177 96
AhdI GACnn_n'nnGTC
Cuts at: 0 150 2115
Size: 150 1965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1304 2115
Size: 1304 811
AlwNI CAG_nnn'CTG
Cuts at: 0 787 1297 2115
Size: 787 510 818
Fragments arranged by size:
818 787 510
ApaI G_GGCC'C
Cuts at: 0 225 299 2115
Size: 225 74 1816
Fragments arranged by size:
1816 225 74
ApoI r'AATT_y
Cuts at: 0 835 2016 2115
Size: 835 1181 99
Fragments arranged by size:
1181 835 99
AseI AT'TA_AT
Cuts at: 0 2045 2115
Size: 2045 70
AvaI C'yCGr_G
Cuts at: 0 607 681 2115
Size: 607 74 1434
Fragments arranged by size:
1434 607 74
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 511 544 2115
Size: 511 33 1571
Fragments arranged by size:
1571 511 33
BanI G'GyrC_C
Cuts at: 0 510 685 2115
Size: 510 175 1430
Fragments arranged by size:
1430 510 175
BanII G_rGCy'C
Cuts at: 0 31 203 225 299 319 421 711 1247
Size: 31 172 22 74 20 102 290 536
Cuts at: 1247 1493 2115
Size: 246 622
Fragments arranged by size:
622 536 290 246 172 102 74 31
22 20
BbeI G_GCGC'C
Cuts at: 0 514 2115
Size: 514 1601
BclI T'GATC_A
Cuts at: 0 860 1335 2115
Size: 860 475 780
Fragments arranged by size:
860 780 475
BglI GCCn_nnn'nGGC
Cuts at: 0 229 314 379 742 828 2115
Size: 229 85 65 363 86 1287
Fragments arranged by size:
1287 363 229 86 85 65
Bme1580I G_kGCm'C
Cuts at: 0 225 299 604 688 801 2115
Size: 225 74 305 84 113 1314
Fragments arranged by size:
1314 305 225 113 84 74
BmgBI CAC'GTC
Cuts at: 0 804 2115
Size: 804 1311
BmrI ACTGGGnnnn_n'
Cuts at: 0 772 2115
Size: 772 1343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 2115
Size: 360 1755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 421 752 1475 1818 1989 2115
Size: 85 336 331 723 343 171 126
Fragments arranged by size:
723 343 336 331 171 126 85
Bpu10I CC'TnA_GC
Cuts at: 0 415 2115
Size: 415 1700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 1496 2115
Size: 918 578 619
Fragments arranged by size:
918 619 578
BsaHI Gr'CG_yC
Cuts at: 0 251 511 2115
Size: 251 260 1604
Fragments arranged by size:
1604 260 251
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1401 1413 1431 1443 2115
Size: 1401 12 18 12 672
Fragments arranged by size:
1401 672 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 116 346 349 2115
Size: 116 230 3 1766
Fragments arranged by size:
1766 230 116 3
BseYI C'CCAG_C
Cuts at: 0 109 505 1601 2115
Size: 109 396 1096 514
Fragments arranged by size:
1096 514 396 109
BsiEI CG_ry'CG
Cuts at: 0 309 2115
Size: 309 1806
BsiHKAI G_wGCw'C
Cuts at: 0 31 421 2115
Size: 31 390 1694
Fragments arranged by size:
1694 390 31
Bsp1286I G_dGCh'C
Cuts at: 0 31 203 225 299 319 421 604 688
Size: 31 172 22 74 20 102 183 84
Cuts at: 688 711 801 1247 1493 2115
Size: 23 90 446 246 622
Fragments arranged by size:
622 446 246 183 172 102 90 84
74 31 23 22 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 2115
Size: 47 2068
BsrBI CCG'CTC
Cuts at: 0 194 426 714 2115
Size: 194 232 288 1401
Fragments arranged by size:
1401 288 232 194
BsrDI GCAATG_nn'
Cuts at: 0 1286 1687 1775 2115
Size: 1286 401 88 340
Fragments arranged by size:
1286 401 340 88
BsrFI r'CCGG_y
Cuts at: 0 634 818 2115
Size: 634 184 1297
Fragments arranged by size:
1297 634 184
BsrGI T'GTAC_A
Cuts at: 0 1382 2115
Size: 1382 733
BssHII G'CGCG_C
Cuts at: 0 47 394 547 2115
Size: 47 347 153 1568
Fragments arranged by size:
1568 347 153 47
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1297 2115
Size: 1297 818
BstEII G'GTnAC_C
Cuts at: 0 1002 2115
Size: 1002 1113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1633 2115
Size: 1633 482
BstYI r'GATC_y
Cuts at: 0 748 1435 2115
Size: 748 687 680
Fragments arranged by size:
748 687 680
BstZ17I GTA'TAC
Cuts at: 0 1962 2115
Size: 1962 153
Bsu36I CC'TnA_GG
Cuts at: 0 452 2115
Size: 452 1663
BtgI C'CryG_G
Cuts at: 0 227 377 404 516 590 1421 1825 2115
Size: 227 150 27 112 74 831 404 290
Fragments arranged by size:
831 404 290 227 150 112 74 27
BtsI GCAGTG_nn'
Cuts at: 0 287 389 651 926 1298 2115
Size: 287 102 262 275 372 817
Fragments arranged by size:
817 372 287 275 262 102
EaeI y'GGCC_r
Cuts at: 0 99 230 284 306 386 1589 2115
Size: 99 131 54 22 80 1203 526
Fragments arranged by size:
1203 526 131 99 80 54 22
EagI C'GGCC_G
Cuts at: 0 306 2115
Size: 306 1809
EarI CTCTTCn'nnn_
Cuts at: 0 490 1577 1908 2048 2115
Size: 490 1087 331 140 67
Fragments arranged by size:
1087 490 331 140 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 336 979 2115
Size: 336 643 1136
Fragments arranged by size:
1136 643 336
EcoICRI GAG'CTC
Cuts at: 0 29 419 2115
Size: 29 390 1696
Fragments arranged by size:
1696 390 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 421 752 1344 1376 1475 1705 1818
Size: 85 336 331 592 32 99 230 113
Cuts at: 1818 1880 1989 2072 2115
Size: 62 109 83 43
Fragments arranged by size:
592 336 331 230 113 109 99 85
83 62 43 32
EcoO109I rG'GnC_Cy
Cuts at: 0 221 222 295 296 768 2115
Size: 221 1 73 1 472 1347
Fragments arranged by size:
1347 472 221 73 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 2115
Size: 156 1959
HaeII r_GCGC'y
Cuts at: 0 98 275 514 984 2115
Size: 98 177 239 470 1131
Fragments arranged by size:
1131 470 239 177 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 1855 1887 2115
Size: 136 32 1687 32 228
Fragments arranged by size:
1687 228 136 32 32
HincII GTy'rAC
Cuts at: 0 1409 1500 2115
Size: 1409 91 615
Fragments arranged by size:
1409 615 91
HpaI GTT'AAC
Cuts at: 0 1409 2115
Size: 1409 706
KasI G'GCGC_C
Cuts at: 0 510 2115
Size: 510 1605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 440 2115
Size: 116 324 1675
Fragments arranged by size:
1675 324 116
MscI TGG'CCA
Cuts at: 0 101 286 1591 2115
Size: 101 185 1305 524
Fragments arranged by size:
1305 524 185 101
MslI CAynn'nnrTG
Cuts at: 0 938 1156 1291 2115
Size: 938 218 135 824
Fragments arranged by size:
938 824 218 135
MspA1I CmG'CkG
Cuts at: 0 63 86 229 352 379 592 665 742
Size: 63 23 143 123 27 213 73 77
Cuts at: 742 1605 1816 2115
Size: 863 211 299
Fragments arranged by size:
863 299 213 211 143 123 77 73
63 27 23
NarI GG'CG_CC
Cuts at: 0 511 2115
Size: 511 1604
NcoI C'CATG_G
Cuts at: 0 404 1825 2115
Size: 404 1421 290
Fragments arranged by size:
1421 404 290
NdeI CA'TA_TG
Cuts at: 0 1698 2115
Size: 1698 417
NspI r_CATG'y
Cuts at: 0 888 1032 2115
Size: 888 144 1083
Fragments arranged by size:
1083 888 144
PasI CC'CwG_GG
Cuts at: 0 1737 2115
Size: 1737 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 787 977 1737 2115
Size: 787 190 760 378
Fragments arranged by size:
787 760 378 190
PfoI T'CCnGG_A
Cuts at: 0 1484 2115
Size: 1484 631
PsiI TTA'TAA
Cuts at: 0 1526 2115
Size: 1526 589
PspOMI G'GGCC_C
Cuts at: 0 221 295 2115
Size: 221 74 1820
Fragments arranged by size:
1820 221 74
PstI C_TGCA'G
Cuts at: 0 357 2115
Size: 357 1758
PvuII CAG'CTG
Cuts at: 0 1816 2115
Size: 1816 299
SacI G_AGCT'C
Cuts at: 0 31 421 2115
Size: 31 390 1694
Fragments arranged by size:
1694 390 31
SacII CC_GC'GG
Cuts at: 0 230 380 593 2115
Size: 230 150 213 1522
Fragments arranged by size:
1522 230 213 150
SapI GCTCTTCn'nnn_
Cuts at: 0 1908 2115
Size: 1908 207
SfcI C'TryA_G
Cuts at: 0 353 2115
Size: 353 1762
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 229 2115
Size: 229 1886
SfoI GGC'GCC
Cuts at: 0 512 2115
Size: 512 1603
SmaI CCC'GGG
Cuts at: 0 609 683 2115
Size: 609 74 1432
Fragments arranged by size:
1432 609 74
SmlI C'TyrA_G
Cuts at: 0 897 1511 2115
Size: 897 614 604
Fragments arranged by size:
897 614 604
SrfI GCCC'GGGC
Cuts at: 0 683 2115
Size: 683 1432
StyI C'CwwG_G
Cuts at: 0 404 1825 1874 2115
Size: 404 1421 49 241
Fragments arranged by size:
1421 404 241 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 990 1070 1168 2115
Size: 990 80 98 947
Fragments arranged by size:
990 947 98 80
TatI w'GTAC_w
Cuts at: 0 695 1382 2115
Size: 695 687 733
Fragments arranged by size:
733 695 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1413 1445 2115
Size: 1413 32 670
Fragments arranged by size:
1413 670 32
XbaI T'CTAG_A
Cuts at: 0 1938 2115
Size: 1938 177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 502 2115
Size: 502 1613
XmaI C'CCGG_G
Cuts at: 0 607 681 2115
Size: 607 74 1434
Fragments arranged by size:
1434 607 74
Enzymes that do cut:
AccI AcuI AfeI AhdI AlfI AlwNI ApaI ApoI
AseI AvaI BaeI BanI BanII BbeI BclI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI
KasI MmeI MscI MslI MspA1I NarI NcoI NdeI
NspI PasI PflMI PfoI PsiI PspOMI PstI PvuII
SacI SacII SapI SfcI SfiI SfoI SmaI SmlI
SrfI StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AleI
AloI ApaLI AscI AsiSI AvrII BamHI BbsI BbvCI
BcgI BciVI BfrBI BglII BlpI BmtI BsaI BsaAI
BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI
BssSI BstBI BtgZI ClaI CspCI DraI DraIII DrdI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KpnI
MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
Tth111I XhoI XmnI ZraI