Restriction map for
pF1KB9755
(Linear) (Six-base) MAPSORT of: KIBB9755.seq Check: 9953 from: 1 to: 1983
KIBB9755.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 176 1983
Size: 176 1807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1983
Size: 72 1911
AflIII A'CryG_T
Cuts at: 0 823 1983
Size: 823 1160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 541 1983
Size: 509 32 1442
Fragments arranged by size:
1442 509 32
AlwNI CAG_nnn'CTG
Cuts at: 0 47 111 1421 1925 1983
Size: 47 64 1310 504 58
Fragments arranged by size:
1310 504 64 58 47
ApoI r'AATT_y
Cuts at: 0 283 311 520 796 832 881 1023 1217
Size: 283 28 209 276 36 49 142 194
Cuts at: 1217 1301 1385 1469 1617 1721 1805 1869 1983
Size: 84 84 84 148 104 84 64 114
Fragments arranged by size:
283 276 209 194 148 142 114 104
84 84 84 84 64 49 36 28
AseI AT'TA_AT
Cuts at: 0 1033 1983
Size: 1033 950
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 118 151 1983
Size: 118 33 1832
Fragments arranged by size:
1832 118 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1983
Size: 359 1624
BbvCI CC'TCA_GC
Cuts at: 0 763 1983
Size: 763 1220
BfrBI ATG'CAT
Cuts at: 0 1041 1293 1461 1965 1983
Size: 1041 252 168 504 18
Fragments arranged by size:
1041 504 252 168 18
BglII A'GATC_T
Cuts at: 0 842 1983
Size: 842 1141
BlpI GC'TnA_GC
Cuts at: 0 910 1983
Size: 910 1073
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1983
Size: 1168 815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1247 1415 1438 1583 1667 1919 1983
Size: 1163 84 168 23 145 84 252 64
Fragments arranged by size:
1163 252 168 145 84 84 64 23
Bpu10I CC'TnA_GC
Cuts at: 0 763 1983
Size: 763 1220
BsaHI Gr'CG_yC
Cuts at: 0 129 1983
Size: 129 1854
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 321 863 1983
Size: 112 209 542 1120
Fragments arranged by size:
1120 542 209 112
BseYI C'CCAG_C
Cuts at: 0 23 1983
Size: 23 1960
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 1983
Size: 1856 127
BsmI GAATG_Cn'
Cuts at: 0 1165 1417 1983
Size: 1165 252 566
Fragments arranged by size:
1165 566 252
BspHI T'CATG_A
Cuts at: 0 903 1983
Size: 903 1080
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 1983
Size: 176 1807
BsrDI GCAATG_nn'
Cuts at: 0 639 1086 1983
Size: 639 447 897
Fragments arranged by size:
897 639 447
BstBI TT'CG_AA
Cuts at: 0 185 1983
Size: 185 1798
BstYI r'GATC_y
Cuts at: 0 842 1983
Size: 842 1141
BtgI C'CryG_G
Cuts at: 0 235 1983
Size: 235 1748
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1951 1983
Size: 1951 32
BtsI GCAGTG_nn'
Cuts at: 0 48 1170 1254 1422 1983
Size: 48 1122 84 168 561
Fragments arranged by size:
1122 561 168 84 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1068 1103 1983
Size: 1068 35 880
Fragments arranged by size:
1068 880 35
EarI CTCTTCn'nnn_
Cuts at: 0 583 696 1983
Size: 583 113 1287
Fragments arranged by size:
1287 583 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1163 1247 1415 1438 1583 1667 1919
Size: 72 1091 84 168 23 145 84 252
Cuts at: 1919 1983
Size: 64
Fragments arranged by size:
1091 252 168 145 84 84 72 64
23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 1983
Size: 136 1847
EcoO109I rG'GnC_Cy
Cuts at: 0 1319 1682 1983
Size: 1319 363 301
Fragments arranged by size:
1319 363 301
EcoRI G'AATT_C
Cuts at: 0 1023 1301 1385 1469 1721 1805 1983
Size: 1023 278 84 84 252 84 178
Fragments arranged by size:
1023 278 252 178 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1383 1983
Size: 1383 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1362 1394 1698 1730 1983
Size: 1362 32 304 32 253
Fragments arranged by size:
1362 304 253 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 197 1983
Size: 131 66 1786
Fragments arranged by size:
1786 131 66
MslI CAynn'nnrTG
Cuts at: 0 41 572 974 1830 1983
Size: 41 531 402 856 153
Fragments arranged by size:
856 531 402 153 41
NcoI C'CATG_G
Cuts at: 0 235 1983
Size: 235 1748
NdeI CA'TA_TG
Cuts at: 0 991 1983
Size: 991 992
NsiI A_TGCA'T
Cuts at: 0 1043 1295 1463 1967 1983
Size: 1043 252 168 504 16
Fragments arranged by size:
1043 504 252 168 16
NspI r_CATG'y
Cuts at: 0 827 1983
Size: 827 1156
PciI A'CATG_T
Cuts at: 0 823 1983
Size: 823 1160
PpuMI rG'GwC_Cy
Cuts at: 0 1319 1983
Size: 1319 664
PsiI TTA'TAA
Cuts at: 0 1745 1983
Size: 1745 238
SfcI C'TryA_G
Cuts at: 0 159 1730 1983
Size: 159 1571 253
Fragments arranged by size:
1571 253 159
SspI AAT'ATT
Cuts at: 0 479 1974 1983
Size: 479 1495 9
Fragments arranged by size:
1495 479 9
StuI AGG'CCT
Cuts at: 0 1012 1348 1600 1983
Size: 1012 336 252 383
Fragments arranged by size:
1012 383 336 252
StyI C'CwwG_G
Cuts at: 0 235 1983
Size: 235 1748
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 865 1957 1983
Size: 865 1092 26
Fragments arranged by size:
1092 865 26
TatI w'GTAC_w
Cuts at: 0 826 994 1330 1794 1983
Size: 826 168 336 464 189
Fragments arranged by size:
826 464 336 189 168
XmnI GAAnn'nnTTC
Cuts at: 0 844 1012 1432 1516 1600 1768 1852 1983
Size: 844 168 420 84 84 168 84 131
Fragments arranged by size:
844 420 168 168 131 84 84 84
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApoI AseI BaeI
BbsI BbvCI BfrBI BglII BlpI BplI BpmI Bpu10I
BsaHI BseRI BseYI BsgI BsmI BspHI BspMI BsrDI
BstBI BstYI BtgI BtgZI BtsI CspCI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I MmeI MslI NcoI
NdeI NsiI NspI PciI PpuMI PsiI SfcI SspI
StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
Bme1580I BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI