Restriction map for
pF1KB9751
(Linear) (Six-base) MAPSORT of: KIBB9751.seq Check: 8635 from: 1 to: 1818
KIBB9751.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
Acc65I G'GTAC_C
Cuts at: 0 900 1818
Size: 900 918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 1419 1818
Size: 918 501 399
Fragments arranged by size:
918 501 399
AfeI AGC'GCT
Cuts at: 0 1276 1818
Size: 1276 542
ApoI r'AATT_y
Cuts at: 0 52 163 186 402 677 985 1023 1205
Size: 52 111 23 216 275 308 38 182
Cuts at: 1205 1289 1709 1818
Size: 84 420 109
Fragments arranged by size:
420 308 275 216 182 111 109 84
52 38 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 320 353 1818
Size: 320 33 1465
Fragments arranged by size:
1465 320 33
BanI G'GyrC_C
Cuts at: 0 220 900 1818
Size: 220 680 918
Fragments arranged by size:
918 680 220
BanII G_rGCy'C
Cuts at: 0 862 1195 1818
Size: 862 333 623
Fragments arranged by size:
862 623 333
BbeI G_GCGC'C
Cuts at: 0 224 1818
Size: 224 1594
BbsI GAAGACnn'nnnn_
Cuts at: 0 626 943 1256 1818
Size: 626 317 313 562
Fragments arranged by size:
626 562 317 313
BclI T'GATC_A
Cuts at: 0 1773 1818
Size: 1773 45
BfrBI ATG'CAT
Cuts at: 0 919 1818
Size: 919 899
BglII A'GATC_T
Cuts at: 0 941 1365 1818
Size: 941 424 453
Fragments arranged by size:
941 453 424
BlpI GC'TnA_GC
Cuts at: 0 1698 1818
Size: 1698 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 568 1818
Size: 297 271 1250
Fragments arranged by size:
1250 297 271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 751 1818
Size: 751 1067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1499 1818
Size: 746 753 319
Fragments arranged by size:
753 746 319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 1818
Size: 425 1393
BsaHI Gr'CG_yC
Cuts at: 0 221 1818
Size: 221 1597
BsaWI w'CCGG_w
Cuts at: 0 862 1818
Size: 862 956
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1818
Size: 79 30 1709
Fragments arranged by size:
1709 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 1818
Size: 58 1760
BseYI C'CCAG_C
Cuts at: 0 1271 1818
Size: 1271 547
BsiHKAI G_wGCw'C
Cuts at: 0 862 1195 1818
Size: 862 333 623
Fragments arranged by size:
862 623 333
BsmI GAATG_Cn'
Cuts at: 0 219 561 1373 1818
Size: 219 342 812 445
Fragments arranged by size:
812 445 342 219
Bsp1286I G_dGCh'C
Cuts at: 0 862 1195 1818
Size: 862 333 623
Fragments arranged by size:
862 623 333
BspEI T'CCGG_A
Cuts at: 0 862 1818
Size: 862 956
BspHI T'CATG_A
Cuts at: 0 1584 1818
Size: 1584 234
BstBI TT'CG_AA
Cuts at: 0 1203 1707 1818
Size: 1203 504 111
Fragments arranged by size:
1203 504 111
BstYI r'GATC_y
Cuts at: 0 941 1365 1818
Size: 941 424 453
Fragments arranged by size:
941 453 424
BtgI C'CryG_G
Cuts at: 0 530 1818
Size: 530 1288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 190 225 1818
Size: 190 35 1593
Fragments arranged by size:
1593 190 35
DraI TTT'AAA
Cuts at: 0 1074 1818
Size: 1074 744
DraIII CAC_nnn'GTG
Cuts at: 0 700 1818
Size: 700 1118
EarI CTCTTCn'nnn_
Cuts at: 0 974 1496 1709 1800 1818
Size: 974 522 213 91 18
Fragments arranged by size:
974 522 213 91 18
EcoICRI GAG'CTC
Cuts at: 0 860 1193 1818
Size: 860 333 625
Fragments arranged by size:
860 625 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 918 1419 1499 1818
Size: 746 172 501 80 319
Fragments arranged by size:
746 501 319 172 80
EcoRI G'AATT_C
Cuts at: 0 1205 1709 1818
Size: 1205 504 109
Fragments arranged by size:
1205 504 109
HaeII r_GCGC'y
Cuts at: 0 224 1278 1818
Size: 224 1054 540
Fragments arranged by size:
1054 540 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 933 965 1086 1118 1182 1214 1818
Size: 933 32 121 32 64 32 604
Fragments arranged by size:
933 604 121 64 32 32 32
HincII GTy'rAC
Cuts at: 0 840 1818
Size: 840 978
HindIII A'AGCT_T
Cuts at: 0 1450 1818
Size: 1450 368
KasI G'GCGC_C
Cuts at: 0 220 1818
Size: 220 1598
KpnI G_GTAC'C
Cuts at: 0 904 1818
Size: 904 914
MslI CAynn'nnrTG
Cuts at: 0 1158 1589 1818
Size: 1158 431 229
Fragments arranged by size:
1158 431 229
NarI GG'CG_CC
Cuts at: 0 221 1818
Size: 221 1597
NcoI C'CATG_G
Cuts at: 0 530 1818
Size: 530 1288
NsiI A_TGCA'T
Cuts at: 0 921 1818
Size: 921 897
NspI r_CATG'y
Cuts at: 0 764 923 1818
Size: 764 159 895
Fragments arranged by size:
895 764 159
PflMI CCAn_nnn'nTGG
Cuts at: 0 346 1818
Size: 346 1472
PfoI T'CCnGG_A
Cuts at: 0 56 514 1818
Size: 56 458 1304
Fragments arranged by size:
1304 458 56
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 533 565 1818
Size: 533 32 1253
Fragments arranged by size:
1253 533 32
PshAI GACnn'nnGTC
Cuts at: 0 1352 1818
Size: 1352 466
PsiI TTA'TAA
Cuts at: 0 1241 1325 1763 1818
Size: 1241 84 438 55
Fragments arranged by size:
1241 438 84 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1106 1138 1818
Size: 1106 32 680
Fragments arranged by size:
1106 680 32
SacI G_AGCT'C
Cuts at: 0 862 1195 1818
Size: 862 333 623
Fragments arranged by size:
862 623 333
SapI GCTCTTCn'nnn_
Cuts at: 0 1709 1818
Size: 1709 109
ScaI AGT'ACT
Cuts at: 0 652 1115 1818
Size: 652 463 703
Fragments arranged by size:
703 652 463
SfcI C'TryA_G
Cuts at: 0 81 1818
Size: 81 1737
SfoI GGC'GCC
Cuts at: 0 222 1818
Size: 222 1596
SmlI C'TyrA_G
Cuts at: 0 440 1818
Size: 440 1378
SphI G_CATG'C
Cuts at: 0 764 1818
Size: 764 1054
SspI AAT'ATT
Cuts at: 0 1759 1818
Size: 1759 59
StyI C'CwwG_G
Cuts at: 0 412 530 1818
Size: 412 118 1288
Fragments arranged by size:
1288 412 118
TatI w'GTAC_w
Cuts at: 0 650 1113 1218 1470 1818
Size: 650 463 105 252 348
Fragments arranged by size:
650 463 348 252 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 1818
Size: 79 32 1707
Fragments arranged by size:
1707 79 32
XmnI GAAnn'nnTTC
Cuts at: 0 257 587 1432 1818
Size: 257 330 845 386
Fragments arranged by size:
845 386 330 257
Enzymes that do cut:
Acc65I AcuI AfeI ApoI BaeI BanI BanII BbeI
BbsI BclI BfrBI BglII BlpI BmrI BplI BpmI
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspEI BspHI BstBI BstYI BtgI CspCI DraI
DraIII EarI EcoICRI Eco57MI EcoRI HaeII Hin4I HincII
HindIII KasI KpnI MslI NarI NcoI NsiI NspI
PflMI PfoI PpiI PshAI PsiI PsrI SacI SapI
ScaI SfcI SfoI SmlI SphI SspI StyI TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI BglI
Bme1580I BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsgI
BsiEI BsiWI BsmBI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI DrdI EaeI EagI EciI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HpaI MfeI MluI
MmeI MscI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI ZraI