Restriction map for
pF1KB9742
(Linear) (Six-base) MAPSORT of: KIBB9742.seq Check: 6648 from: 1 to: 1563
KIBB9742.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AfeI AGC'GCT
Cuts at: 0 628 1563
Size: 628 935
AflII C'TTAA_G
Cuts at: 0 738 1563
Size: 738 825
AflIII A'CryG_T
Cuts at: 0 756 952 1062 1563
Size: 756 196 110 501
Fragments arranged by size:
756 501 196 110
AleI CACnn'nnGTG
Cuts at: 0 331 1563
Size: 331 1232
ApaLI G'TGCA_C
Cuts at: 0 161 225 795 1563
Size: 161 64 570 768
Fragments arranged by size:
768 570 161 64
AvaI C'yCGr_G
Cuts at: 0 429 1563
Size: 429 1134
BanI G'GyrC_C
Cuts at: 0 269 433 1563
Size: 269 164 1130
Fragments arranged by size:
1130 269 164
BanII G_rGCy'C
Cuts at: 0 97 110 1563
Size: 97 13 1453
Fragments arranged by size:
1453 97 13
BbeI G_GCGC'C
Cuts at: 0 273 1563
Size: 273 1290
BclI T'GATC_A
Cuts at: 0 114 1563
Size: 114 1449
BfrBI ATG'CAT
Cuts at: 0 980 1563
Size: 980 583
BglI GCCn_nnn'nGGC
Cuts at: 0 851 1563
Size: 851 712
Bme1580I G_kGCm'C
Cuts at: 0 165 229 436 799 1563
Size: 165 64 207 363 764
Fragments arranged by size:
764 363 207 165 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 474 491 1237 1381 1411 1563
Size: 437 37 17 746 144 30 152
Fragments arranged by size:
746 437 152 144 37 30 17
Bpu10I CC'TnA_GC
Cuts at: 0 91 1563
Size: 91 1472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1563
Size: 708 855
BsaI GGTCTCn'nnnn_
Cuts at: 0 390 671 985 1500 1563
Size: 390 281 314 515 63
Fragments arranged by size:
515 390 314 281 63
BsaHI Gr'CG_yC
Cuts at: 0 270 1563
Size: 270 1293
BsaWI w'CCGG_w
Cuts at: 0 644 1563
Size: 644 919
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 1563
Size: 565 30 968
Fragments arranged by size:
968 565 30
BseYI C'CCAG_C
Cuts at: 0 358 1563
Size: 358 1205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1316 1563
Size: 911 405 247
Fragments arranged by size:
911 405 247
BsiHKAI G_wGCw'C
Cuts at: 0 97 165 229 799 1563
Size: 97 68 64 570 764
Fragments arranged by size:
764 570 97 68 64
Bsp1286I G_dGCh'C
Cuts at: 0 97 110 165 229 436 799 1563
Size: 97 13 55 64 207 363 764
Fragments arranged by size:
764 363 207 97 64 55 13
BspHI T'CATG_A
Cuts at: 0 669 1563
Size: 669 894
BsrDI GCAATG_nn'
Cuts at: 0 124 392 1268 1508 1563
Size: 124 268 876 240 55
Fragments arranged by size:
876 268 240 124 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 233 1563
Size: 233 1330
BstXI CCAn_nnnn'nTGG
Cuts at: 0 333 1563
Size: 333 1230
Bsu36I CC'TnA_GG
Cuts at: 0 155 265 1563
Size: 155 110 1298
Fragments arranged by size:
1298 155 110
BtgI C'CryG_G
Cuts at: 0 101 329 1163 1563
Size: 101 228 834 400
Fragments arranged by size:
834 400 228 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 339 1563
Size: 339 1224
BtsI GCAGTG_nn'
Cuts at: 0 456 708 797 1409 1563
Size: 456 252 89 612 154
Fragments arranged by size:
612 456 252 154 89
DraIII CAC_nnn'GTG
Cuts at: 0 840 1563
Size: 840 723
EaeI y'GGCC_r
Cuts at: 0 310 1075 1563
Size: 310 765 488
Fragments arranged by size:
765 488 310
EarI CTCTTCn'nnn_
Cuts at: 0 983 1563
Size: 983 580
EcoICRI GAG'CTC
Cuts at: 0 95 1563
Size: 95 1468
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 1563
Size: 28 1535
HaeII r_GCGC'y
Cuts at: 0 273 630 1563
Size: 273 357 933
Fragments arranged by size:
933 357 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 1563
Size: 563 32 968
Fragments arranged by size:
968 563 32
HincII GTy'rAC
Cuts at: 0 410 1302 1392 1563
Size: 410 892 90 171
Fragments arranged by size:
892 410 171 90
HindIII A'AGCT_T
Cuts at: 0 41 242 1538 1563
Size: 41 201 1296 25
Fragments arranged by size:
1296 201 41 25
HpaI GTT'AAC
Cuts at: 0 1302 1563
Size: 1302 261
KasI G'GCGC_C
Cuts at: 0 269 1563
Size: 269 1294
MscI TGG'CCA
Cuts at: 0 1077 1563
Size: 1077 486
MslI CAynn'nnrTG
Cuts at: 0 222 331 466 524 590 1471 1563
Size: 222 109 135 58 66 881 92
Fragments arranged by size:
881 222 135 109 92 66 58
MspA1I CmG'CkG
Cuts at: 0 1258 1563
Size: 1258 305
NarI GG'CG_CC
Cuts at: 0 270 1563
Size: 270 1293
NsiI A_TGCA'T
Cuts at: 0 982 1563
Size: 982 581
NspI r_CATG'y
Cuts at: 0 760 956 980 1066 1524 1563
Size: 760 196 24 86 458 39
Fragments arranged by size:
760 458 196 86 39 24
PciI A'CATG_T
Cuts at: 0 756 952 1062 1563
Size: 756 196 110 501
Fragments arranged by size:
756 501 196 110
PstI C_TGCA'G
Cuts at: 0 238 1563
Size: 238 1325
PvuII CAG'CTG
Cuts at: 0 1258 1563
Size: 1258 305
SacI G_AGCT'C
Cuts at: 0 97 1563
Size: 97 1466
SfcI C'TryA_G
Cuts at: 0 234 1511 1563
Size: 234 1277 52
Fragments arranged by size:
1277 234 52
SfoI GGC'GCC
Cuts at: 0 271 1563
Size: 271 1292
SmlI C'TyrA_G
Cuts at: 0 723 738 1563
Size: 723 15 825
Fragments arranged by size:
825 723 15
SphI G_CATG'C
Cuts at: 0 1524 1563
Size: 1524 39
StuI AGG'CCT
Cuts at: 0 544 729 1563
Size: 544 185 834
Fragments arranged by size:
834 544 185
StyI C'CwwG_G
Cuts at: 0 1558 1563
Size: 1558 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 321 353 565 597 1563
Size: 321 32 212 32 966
Fragments arranged by size:
966 321 212 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 480 1233 1563
Size: 480 753 330
Fragments arranged by size:
753 480 330
XmnI GAAnn'nnTTC
Cuts at: 0 544 718 1563
Size: 544 174 845
Fragments arranged by size:
845 544 174
Enzymes that do cut:
AfeI AflII AflIII AleI ApaLI AvaI BanI BanII
BbeI BclI BfrBI BglI Bme1580I BmrI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrDI BstAPI BstXI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI EcoNI HaeII Hin4I HincII
HindIII HpaI KasI MscI MslI MspA1I NarI NsiI
NspI PciI PstI PvuII SacI SfcI SfoI SmlI
SphI StuI StyI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AgeI AhdI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BglII
BlpI BmgBI BmtI BplI BpmI BsaAI BsaBI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I ClaI
CspCI DraI DrdI EagI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII
TatI XbaI XcmI XhoI XmaI ZraI